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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1364-1370.doi: 10.3724/SP.J.1006.2014.01364

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Identification of Alleles on Lpx-B1 locus and Lipoxygenase Activity in Durum Wheat (Triticum turgidum L.)

ZHANG Fu-Yan1,2,**,SHANG Xiao-Li1,**,WU Pei-Pei1,SONG Shuang1,CHEN Feng1,*,CUI Dang-Qun1,*   

  1. 1 College of Agronomy / Collaborative Innovation Center of Henan Grain Crops / National Key Laboratory of Wheat & Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China; 2 Henan Key Laboratory of Nuclear Agricultural Sciences / Isotope Institute Co., Ltd, Henan Academy of Sciences, Zhengzhou 450015, China?
  • Received:2013-11-15 Revised:2014-04-16 Online:2014-08-12 Published:2014-06-03
  • Contact: 陈锋, E-mail: chf0088@gmail.com; 崔党群, E-mail: cdq62@sohu.com

Abstract:

Lipoxygenase (LOX) has a close relationship to the processing quality of flour products in durum wheat (Triticum turgidum L.). Activity of LOX is greatly influenced by the locus of Lpx-B1. The Lpx-B1 alleles in 167 durum wheat varieties from different countries or regions were identified using gene-specific primers, and the Lpx-B1 genotypes were analyzed based on LOX activity data. The LOX activity varied greatly in the 167 varieties, ranging from 0.20 to 7.98 AU min-1 g-1. On Lpx-B1.1 locus, three alleles (Lpx-B1.1a, Lpx-B1.1b, and Lpx-B1.1c) were identified with frequencies of 55.1%, 7.8%, and 37.1%, respectively. Loci Lpx-B1.2 and Lpx-B1.3 were always reciprocally present in varieties, in which Lpx-B1.2 was presented in 146 varieties and Lpx-B1.3 in the remaining 21 varieties. This result indicates the allelic possibility of Lpx-B1.2 and Lpx-B1.3. In the three Lpx-B1.1 genotypes, the Lpx-B1.1b varieties had significantly higher LOX activity than the Lpx-B1.1a and Lpx-B1.1c varieties, and varieties carrying Lpx-B1.1c allele possessed the lowest LOX activity. The LOX activity in Lpx-B1.3 genotypes was significantly higher than that in Lpx-B1.2 genotypes. A total of six genotypic combinations on the three Lpx-B1 loci in 167 varieties. Among them, Lpx-B1.1b/Lpx-B1.3 combination had the highest LOX activity and combinations of Lpx-B1.1c/Lpx-B1.2 and Lpx-B1.1c/Lpx-B1.3 had the lowest LOX activity. These results are informative to quality breeding of durum wheat.

Key words: Triticum turgidum L., Lpx-B1 locus, Alleles, Lipoxygenase

[1]Loiseau J, Vu B L, Macherel M H, Deunff Y L. Seed lipoxygeanses: occurrence and functions. Seed Sci Res, 2001, 11: 199–211



[2]汪仁, 沈文飚, 翟虎渠, 万建民. 植物种子脂氧合酶. 植物生理学通讯, 2005, 41: 388–395



Wang R, Shen W B, Zhai H Q, Wan J M. Plant seed lipoxygenase. Plant Physiol Comm, 2005, 41: 388–395 (in Chinese with English abstract)



[3]Hessler T G, Thomson M J, Benscher D, Nachit M M, Sorrells M E. Association of a lipoxygenase locus, Lpx-B1, with variation in lipoxygenase activity in durum seeds. Crop Sci, 2002, 42: 1695–1700



[4]Shiiba K, Nengishi Y, Okada K, Nagao S. Purification and characterization of lipoxygenase isozymes from wheat germ. Cereal Chem, 1991, 68: 115–122



[5]Pastore D, Trono D, Padalino L, Simone S, Valenti D, Fonzo N D, Passarella S. Inhibition by α-tocopherol and L-aseorbate of linoleate hydroperoxidation and β-carotene bleaching activities in durum wheat semolina. J Cereal Sci, 2000, 31: 41–54



[6]郑文寅, 姚大年, 张文明. 脂肪氧化酶及其在小麦品质改良中的研究与应用. 粮食与饲料工业, 2009, (8): 5–6



Zheng W Y, Yao D N, Zhang W M. Research and application of lipoxygenase for improvement of wheat quality. Cereal Feed Ind, 2009, (8): 5–6 (in Chinese with English abstract)



[7]Borrelli G M, Deleonardis A M, Platani C, Troccoli A. Distribution along durum wheat kernel of the components involved in semolina color. J Cereal Sci, 2008, 48: 494–502



[8]王慧, 郑文寅, 樊宏, 汪帆, 王青, 王冠球, 张文明, 姚大年. 不同小麦品种籽粒中LOX活性及基因型和环境互作分析. 中国粮油学报, 2011, 26 (1): 11–14



Wang H , Zheng W Y, Fan H, Wang F, Wang Q, Wang G Q, Zhang W M, Yao D N. Lipoxygenase activity and its genotype and environment interactions for different wheat varietier. J Chin Cereals Oils Assoc, 2011, 26(1): 11–14 (in Chinese with English abstract)



[9]Leenhardt F, Lyana B, Rocka E, Boussard A, Potus J, Chanliaud E, Remesy C. Genetic variability of carotenoid concentration, and lipoxygenase and peroxidase activities among cultivated wheat species and bread wheat varieties. Eur J Agron, 2006, 25: 170–176



[10]Hart G E, Langston P J. Chromosome location and evolution of isozyme structural genes in hexaploid wheat. Heredity, 1977, 39: 263–277



[11]Hsieh C C, McDonald CE. Isolation of lipoxygenase isoenzymes from ?our of durum wheat endosperm. Cereal Chem, 1984, 61: 392–398



[12]Garbus I, Carrera A D, Dubcovsky J, Echenique V. Physical mapping of durum wheat lipoxygenase genes. J Cereal Sci,2009, 50: 67–73



[13]Psheniehnikova T A, Osipova S V, Permyakova M D, Mitrofanova T N, Trufanov V A, Lohwasser U, Röder M, Börner A. Mapping of quantitative trait loci (QTL) associated with activity of disulfide reductase and lipoxygenase in grain of bread wheat. Russ J Genet, 2008, 44: 567–574



[14]Carrera A, Echenique V, Zhang W, Helguera M, Manthey F, Schrager A, Picca A, Cervigni G, Dubcovsky J. A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improves pasta color in durum wheat (Triticum turgidum ssp. durum). J Cereal Sci, 2007, 45: 67–77



[15]Verlotta A, Simone V D, Mastrangelo A M, Cattivelli L, Papa R, Trono D. In sight into durum wheat Lpx-B1: a small gene family coding for the lipoxygenase responsible for carotenoid bleaching in mature grains. BMC Plant Biol, 2010, 10: 263



[16]Feng B, Dong Z Y, Xu Z B,Wang D W, Wang T. Molecular characterization of a novel type of lipoxygenase (LOX) gene from common wheat (Triticum aestivum L.). Mol Breed, 2012, 30: 113–124



[17]Geng H W, Xia X C, Zhang L P, Qu Y Y, He Z H. Development of functional markers for a lipoxygenase gene TaLox-B1 on chromosome 4BS in common wheat. Crop Sci, 2012, 52: 568–576



[18]Garbus I, Soresi D, Romero J, Echenique V. Identification, mapping and evolutionary course of wheat lipoxygenase-1 genes located on the A genome. J Cereal Sci, 2013, 58: 298–304



[19]Feng B, Dong Z Y, Xu Z B, An X L, Qin H J, Wu N, Wang D W, Wang T. Molecular analysis of lipoxygenase (LOX) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants. J Cereal Sci, 2010, 52: 387–394



[20]Leenhardt F, Lyan B, Rock E, Boussard A, Potus J, Chanliaud E, Remesy C. Wheat lipoxygenase activity induces greater loss of carotenoids than vitamin E during bread making. J Agric Food Chem, 2006, 54: 1710–1715



[21]Borrelli G M, Ficco D B M, Fonzo N D, Fares C. Effects of lipoxygenase and of chemical oxidising agent potassium iodate on rheological properties of durum dough. Int J Food Sci Technol, 2006, 41: 639–645



[22]郑文寅, 汪帆, 司红起, 张文明, 姚大年. 普通小麦籽粒LOX、PPO活性和类胡萝卜素含量变异及对全麦粉色泽的影响. 中国农业科学, 2013, 46: 1087–1094



Zheng W Y, Wang F, Si H Q, Zhang W M, Yao D N. Variations of LOX and PPO activities and carotenoid content as well as their influence on whole flour color in common wheat. Sci Agric Sin, 2013, 46: 1087-1094 (in Chinese with English abstract)



[23]Chen F, He Z H, Xia X C, Lillemo M, Morris C. A new puroindoline b mutation presented in Chinese winter wheat cultivar Jingdong 11. J Cereal Sci, 2005, 42: 267–269



[24]Borrelli G M, Troeeoli A, Fonzo N D, Fares C. Durum wheat lipoxygenase activity and other quality parameters that affect pasta color. Cereal Chem, 1999, 76: 335–340



[25]赵丹, 张玉荣, 林家永, 周显青. 小麦储藏品质评价指标研究进展.粮食与饲料工业, 2012, (2): 10–14



Zhao D, Zhang Y R, Lin J Y, Zhou X Q . Research on quality evaluation of wheat storage. Cereal Feed Ind, 2012, (2): 10–14 (in Chinese with English abstract)



[26]Trufanov V A, Permyakova M D, Pshenichnikova T A, Ermakova M F, Davydov V A, Permyakov A V, Berezovskaya E V. The effect of intercultivar substitution of wheat Triticum aestivum L. chromosomes on lipoxygenase activity and its correlation with the technological properties of flour. Appl Biochem Microbiol, 2007, 43: 91–97



[27]Permyakova M D, Trufanov V A, Pshenichnikova T A, Ermakova M F. Role of lipoxygenase in the determination of wheat grain quality. Appl Biochem Microbiol, 2010, 46: 87–92



[28]?ili? S, Dodig D, Šukalovi? V H-T, Maksimovi? M, Saratli? G, Škrbi? B. Bread and durum wheat compared for antioxidants contents, and lipoxygenase and peroxidase activities. Int J Food Sci Tech, 2010, 45: 1360–1367



[29]Geng H W, Zhang Y, He Z H, Zhang L P, Appels R, Qu Y Y, Xia X C. Molecular markers for tracking variation in lipoxygenase activity in wheat breeding. Mol Breed, 2011, 28: 117–126



[30]Bagge M, Xia X C, L?bberstedt T. Functional markers in wheat. Curr Opin Plant Biol, 2007, 10: 211–216



[31]Liu Y N, He Z H, Appels R, Xia X C. Functional markers in wheat: current status and future prospects. Theor Appl Genet, 2012, 125: 1–10

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