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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (04): 531-538.doi: 10.3724/SP.J.1006.2015.00531

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Phenolic Acid Synthesis of Allelopathic Rice Regulated by OsMYB and Its Weed Inhibition

SHEN Li-Hua1,2,LI Bi-Liang1,REN Yong-Jie1,LI Cheng-Xun1,ZHONG Yong-Jia2,FANG Chang-Xun1,2,LIN Wen-Xiong1,2,*   

  1. 1 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Institute of Agro-ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2014-11-18 Revised:2015-02-06 Online:2015-04-12 Published:2015-03-03

Abstract:

Regulation of gene expression is a vital process in the formation of rice allelopathy. In this study, allelopathic rice PI312777 (Oryza sativa L.) was modified by RNA interference (RNAi) and overexpression(OE) technologies to inhibit or enhance gene expression of OsMYB (CT829537) in PI312777 respectively. The results showed that up-regulation of phenolic synthesis related genes was found in CT829537-OE transgenic PI312777 co-cultured with barnyardgrass (BYG), compared with that in wild type. However, the reverse was true in the CT829537-RNAi transgenic PI312777. Up-regulation of the gene expression in CT829537-OE transgenic PI312777 increased phenolic acids contents in rice root and root exudates, which led to enhance allelopathic inhibition on barnyardgrass. In contrast, decreases of phenolic acids contents and weed inhibition were found in CT829537-RNAi transgenic PI312777. These results implied that CT829537 is responsible for regulating phenolic synthesis in allelopathic rice PI312777 and then enhances allelopathic inhibition on weeds.

Key words: Rice, Allelopathy, Transcription factor, Phenolic acid, Gene expression regulation

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