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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (03): 361-367.doi: 10.3724/SP.J.1006.2016.00361

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Transmission and Genetic Stability of No-homoeologous Small Fragment Wheat–Haynaldia villosa Translocation Chromosomes with Pm21 in Various Cultivar Backgrounds of Common Wheat

WANG Hai-Yan,XIAO Jin,YUAN Chun-Xia,XU Tao,YU Chun-Yan,SUN Hao-Jie,CHEN Pei-Du, WANG Xiu-E*   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement / Cytogenetics Institute, Nanjing Agricultural University / Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, China
  • Received:2015-12-18 Revised:2015-11-20 Online:2016-03-12 Published:2015-12-18
  • Contact: 王秀娥, E-mail: xiuew@njau.edu.cn, Tel: 025-84395308 E-mail:hywang@njau.edu.cn
  • Supported by:

    This study was supported by the National High Technology Research Program (‘863’ Program) of China (2011AA100101, 2011AA10010201), the National Natural Science Foundation of China (31201204), the Fundamental Research Funds for the Central Universities (KYZ201403) and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Abstract:

The powdery mildew resistance gene Pm21 comes from a diploid wheat related species, Haynaldia villosa. Two Pm21-carrying small fragment translocation lines, the terminal translocation line NAU418 and the small interstitial translocation line NAU419, have been developed. Both lines are highly resistant to powdery mildew and serve as new genetic resources for improvement of disease resistance. For understanding the transmission rate of the translocation chromosomes through male and female gametes and the genetic stabilities in different wheat genetic backgrounds, the two translocations were crossed to 12 common wheat varieties from different wheat growing areas of China. The F1 hybrids were then backcrossed as reciprocally. Chromosome configurations of the obtained F1s were analyzed by fluorescence in situ hybridization (FISH) ofthePMC at MI. It was found that the translocation chromosomes formed rod bivalents with their corresponding wheat chromosomes. Test crosses showed that the translocation chromosomes T1AS•1AL-6VS and T4BS•4BL-6VS-4BL could be transmitted to their offspring. The transmission frequency of T1AS 1AL-6VS was higher through female gametes an average of 33.20%, ranging from 0.08% to 50.98% than through male gametes an average of 23.75%, ranging from 0.14% to 45.07%. Similarly, the transmission frequency of T4BS 4BL-6VS-4BL was higher through female gametes an average of 42.90%, ranging from 29.17% to 52.38% than through male gametes an average of 21.45%, ranging from 7.69% to 47.06%. These results show that the translocated chromosomes could be transmitted through male and female gametes, while genetic background has influences on the transmission rate, especially through male gametes.

Key words: Wheat, Haynaldia villosa, Powdery mildew, Small fragment translocation, Gamete transmission frequency

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