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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (03): 353-360.doi: 10.3724/SP.J.1006.2016.00353

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of QTLs for Brown Planthopper Resistance in Indica Rice WD15515

DENG Zhao**,SHI Shao-Jie**,WANG Hui-Ying,SHANG-GUAN Xin-Xin,LIU Bing-Fang,JING Sheng-Li,DU Bo,CHEN Rong-Zhi,ZHU Li-Li,HE Guang-Cun*   

  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China
  • Received:2015-07-08 Revised:2015-11-20 Online:2016-03-12 Published:2015-12-07
  • Contact: 何光存, E-mail: gche@whu.edu.cn, Tel:027-68752384 E-mail:dengzhao@lpht.com.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China(31230060)and the National Basic Key Research Program(973 Program)(2013CBA01403).

Abstract:

Brownplanthopper (BPH) is one of the most destructive insect pests of rice. WD15515 is an indica germplasm highly resistant to BPH. An F2 population was developed from the cross between 9311 and WD15515. One hundred F2 plants were genotyped by using SSR markers and evaluated for BPH-resistance by measuring the honeydew weightsecreted by BPH(HW), the body weight increment (WB) and the body weight increment ratio (WRB). A total of four QTLs for BPH resistance were identified. The qBph2-1, based on HW(honeydew weight), was detected between RM71 and RM6911 on chromosome2, with LOD score of 3.68 and explaining the 11.08% of phenotypic variation. The qBph2-2, based on both WB and WRB, was mapped between RM6911 and RM521 on chromosome 2, with LOD score of 3.31 and 4.05 and explaining 7.81% and 9.38% of the phenotypic variation, respectively. The qBph4, based on HW, WB and WRB, was detected between RM16996 and RM17075 on chromosome 4, with LOD score of 11.11, 13.81, and 15.41 and explaining 44.38%, 45.24%, and 52.40% of the phenotypic variation, respectively. The qBph9, based on HW, WB, and WRB, was detected onchromosome 9 between RM219 and RM6444, with LOD score of 2.59,4.04, and 3.63. This locus explained 10.91%,12.39%, and 10.01% of the phenotypic variation in this population, respectively. qBph4 is amajorgene for BPH-resistance. This result provides thenew resources for BPH-resistance breeding.

Key words: Rice, Resistance, Brown planthopper, QTL, Genetic map

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