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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (03): 353-360.doi: 10.3724/SP.J.1006.2016.00353

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of QTLs for Brown Planthopper Resistance in Indica Rice WD15515

DENG Zhao**,SHI Shao-Jie**,WANG Hui-Ying,SHANG-GUAN Xin-Xin,LIU Bing-Fang,JING Sheng-Li,DU Bo,CHEN Rong-Zhi,ZHU Li-Li,HE Guang-Cun*   

  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China
  • Received:2015-07-08 Revised:2015-11-20 Online:2016-03-12 Published:2015-12-07
  • Contact: 何光存, E-mail: gche@whu.edu.cn, Tel:027-68752384 E-mail:dengzhao@lpht.com.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China(31230060)and the National Basic Key Research Program(973 Program)(2013CBA01403).

Abstract:

Brownplanthopper (BPH) is one of the most destructive insect pests of rice. WD15515 is an indica germplasm highly resistant to BPH. An F2 population was developed from the cross between 9311 and WD15515. One hundred F2 plants were genotyped by using SSR markers and evaluated for BPH-resistance by measuring the honeydew weightsecreted by BPH(HW), the body weight increment (WB) and the body weight increment ratio (WRB). A total of four QTLs for BPH resistance were identified. The qBph2-1, based on HW(honeydew weight), was detected between RM71 and RM6911 on chromosome2, with LOD score of 3.68 and explaining the 11.08% of phenotypic variation. The qBph2-2, based on both WB and WRB, was mapped between RM6911 and RM521 on chromosome 2, with LOD score of 3.31 and 4.05 and explaining 7.81% and 9.38% of the phenotypic variation, respectively. The qBph4, based on HW, WB and WRB, was detected between RM16996 and RM17075 on chromosome 4, with LOD score of 11.11, 13.81, and 15.41 and explaining 44.38%, 45.24%, and 52.40% of the phenotypic variation, respectively. The qBph9, based on HW, WB, and WRB, was detected onchromosome 9 between RM219 and RM6444, with LOD score of 2.59,4.04, and 3.63. This locus explained 10.91%,12.39%, and 10.01% of the phenotypic variation in this population, respectively. qBph4 is amajorgene for BPH-resistance. This result provides thenew resources for BPH-resistance breeding.

Key words: Rice, Resistance, Brown planthopper, QTL, Genetic map

[1]HeG C, DuB, ChenR Z. Insect resistance. In:ZhangQ F,WingedsR A, eds.Genetics and Genomicsof Rice. New York: Springer, 2013.p399

[2]翟保平. 稻飞虱: 国际视野下的中国问题. 应用昆虫学报, 2011, 48: 1184–1193

Zhai B P. Rice planthoppers: A China problem under the international perspectives. Chin J Appl Entomol, 2011, 48: 1184–1193 (in Chinese with English abstract).

[3]Cheng XY, Zhu LL, He GC. Towards understanding of molecular interactions between rice and the brown planthopper. Mol Plant, 2013, 6: 621–634

[4]Du B, Zhang W L, Liu B F, Hu J, Wei Z, Shi Z Y, He R F, Zhu L L, Chen R Z, Han B, He G C.Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice. Proc Natl Acad Sci USA, 2009, 106: 22163–22168.

[5]Tamura Y, Hattori M, Yoshioka H, Yoshioka M, Takahashi A, Wu J Z, Sentoku N, Yasui H. Map-based cloning and characterization of a brown planthopper resistance gene BPH26 fromOryza sativa L. ssp. indica cultivar ADR52. Sci Rep, 2014, 4:5872

[6]Liu Y, Wu H, Chen H, Wu H, Chen H, Liu Y L, He J, Kang H Y, Sun Z G, Pan G, Wang Q, Hu J L, Zhou F, Zhou K N, Zheng X M, Ren Y L, Chen L M, Wang Y H, Zhao Z G, Lin Q B, Wu F Q, Zhang X, Guo X P, Cheng X N, Jiang L, Wu C Y, Wang H Y, Wan J M. A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insectresistance in rice. NatBiotechnol, 2015, 33: 301–305

[7]Cohen M B, Alam S N, Medina E B, Bernal C C. Brownplanthopper, Nilaparvatalugens, resistance in rice cultivar IR64: mechanism and role in successful N. lugens management in Central Luzon, Philippines. EntomolExpAppl, 1997, 85: 221–229

[8]Huang Z, He G C, Shu L H, Li X H, Zhang Q F. Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet, 2001, 102: 929–934

[9]Xu X F, Mei H W, Luo L J, Chen X N, Li Z K. RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvatalugens). Theor Appl Genet, 2002, 104: 248–253

[10]苏昌潮, 程遐年, 翟虎渠,万建民. 利用回交重组自交群体检测水稻抗褐飞虱数量性状基因座. 遗传学报, 2002, 29: 332–338

Su C C, Chen X N, Zhai H Q, Wan J M. Detection and analysis of QTL for resistance to the brown planthopper, Nilaparvatalugens (Stal), in rice (Oryzasativa L.), using backcross inbred lines.ActaGenetSin, 2002, 29: 332–338 (in Chinese with English abstract)

[11]王建康. 数量性状基因的完备区间作图方法. 作物学报, 2009, 35: 239–245

Wang J K. Inclusive composite interval mapping of quantitative trait genes.ActaAgronSin, 2009, 35: 239–245 (in Chinese with English abstract)

[12]李慧慧, 张鲁燕, 王建康. 数量性状基因定位研究中若干常见问题的分析与解答. 作物学报, 2010, 36: 918–931

Li H H, Zhang L Y, Wang J K. Analysis and answers to frequently asked questions in quantitative trait locus mapping. ActaAgronSin, 2010, 36: 918–931 (in Chinese with English abstract).

[13]Kosambi D D. The estimation of map distances from recombination values. Ann Eugenics, 1994, 12: 172–175

[14]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14(11): 11-13

[15]McCouch S R, Teytelman L, Xu Y, Lobos K B, Clare K, Walton M, Fu B Y, Maghirang R, Li Z K, Xing Y Z. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res, 2002, 9(6): 199–207

[16]刘光杰, 郑宜才, 桂丽琴, 沈君辉. 水稻品种抗稻飞虱鉴定方法的比较研究. 浙江农业学报, 1999, 11: 306–310

Liu G J, Zheng Y C, Gui L Q, Shen J H. A comparative study of the rice planthopper resistance identification method.Zhejiang Agric Sci, 1999, 11:306–310(in Chinese)

[17]刘国庆,颜辉煌,傅强,钱前,张志涛,翟文学,朱立煌. 栽培稻的紧穗野生稻抗褐风虱主效基因的遗传定位. 科学通报, 2001, 46: 738–742

Liu G Q, Yan H H, Fu Q, Qian Q, Zhan Z T, Zhai W X, Zhu L H. Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryzaeichingeri. Chin Sci Bull, 2001, 46:1495–1462 (in Chinese)

[18]Ali M P, Chowdhury T R. Tagging andmapping of genes and QTLs of Nilaparvatalugens resistance in rice. Euphytica, 2014, 195: 1–30

[19]Qiu Y F, Guo J P, Jing S L, Zhu L L, He G C. Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6. Theor Appl Genet, 2012, 124: 485–494

[20]Yang H Y, You A Q, Yang Z F, Zhang F, He R F, Zhu L L, He G C. High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.).Theor Appl Genet, 2004, 110: 182–191

[21]Sun L H, Su C C, Wang C M, Zhai H Q, Wan J M. Mapping of a major resistance gene to the brown planthopper in the rice cultivar RathuHeenati. BreedSci, 2005, 55: 391–396

[22]Rahman M L, Jiang W, Chu S H, Qiao Y, Ham T H, Woo M O, Lee J, Khanam M S, Chin J H, Jeung J U. High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryzaminuta. Theor Appl Genet, 2009, 119: 1237–1246

[23]Hirabayashi H, Ogawa T. Identification and utilization of DNA markers linked to genes for reistance to brown planthopper (BPH) in rice. Adv Breed(Japan), 1999,41:71–74

[24]Qiu Y F, Guo J P, Jing S L, Zhu L L, He G C. High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds. Theor Appl Genet, 2010, 121: 1601–1611

[25]Li R B, Li L S, Wei S M, Wei Y P, Chen Y Z, Bai D L, Yang L, Huang F K, Lu W L, Zhang X J, Li X Y, Yang X Q, Wei Y W. The evaluation and utilization of new genes for brown planthopper resistance in common wild rice (OryzarufipogonGriff.). Mol Plant Breed, 2006, 4:365–371

[26]Huang D, Qiu Y, Zhang Y, Huang F, Meng J, Wei S, Li R, Chen B. Fine mapping and characterization of BPH27, a brown planthopper resistance gene from wild rice (OryzarufipogonGriff.). Theor Appl Genet, 2013, 126: 219–229

[27]He J, Liu Y, Liu Y Q, Liu Y L, Jiang L, Wu H, Kang H Y, Liu S J, Chen L M, Liu X, Cheng X N, Wan J M. High-resolution mapping of brown planthopper (BPH) resistance gene Bph27(t) in rice (Oryza sativa L.). MolBreed, 2013, 31: 549–557

[28]Mei M, Zhuang C, Wan R, Wu J, Hu W, Kochert G. Genetic analysis and tagging of gene for brown planthopper resistance in indicarice. In:Rice Genetics III. Proceedings of the Third International Rice Genetics Symposium.IRRI, Los Baños, Philippines, 1996. pp16–20

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