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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (11): 1721-1726.doi: 10.3724/SP.J.1006.2016.01700

• RESEARCH NOTES • Previous Articles     Next Articles

AWheat DELLA Gain-of-Function Mutant Aibian 1 Promotes Seedling Salt Tolerance

WANG Run-Qing1,2,**, FAN Xiao-Cong2,3**, SONG Mei-Fang2,4, XIAO Yang5, GUO Lin2, MENG Fan-Hua2, YANG Qing-Hua3, WU Da-Fu1,*, and YANG Jian-Ping1,2,*   

  1. 1 School of Life Science & Technology, Henan Institute of Science and Technology, Xinxiang 453000, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Agronomy, Henan Agricultural University / Collaborative Innovation Center of Henan Grain Crops, Zhengzhou 450002, China; 4 Beijing Radiation Center, Beijing 100875, China; 5 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-02-23 Revised:2016-07-11 Online:2016-11-12 Published:2016-08-11
  • Contact: Yang Jianping,E-mail:yangjianping02@caas.cn,Tel:010-82105859;Wu Dafu,E-mail:uau9393@163.com,Tel:0373-3693629 E-mail:wrq1990520@163.com
  • Supported by:

    ThisstudywassupportedbytheKeyProjectofBeijingNaturalScienceFoundation(6151002),theGeneticallyModifiedOrganismBreedingProjectsofChina(2016ZX08010002-003-002),theSpecialprojectofnationalkeyresearchanddevelopmentproject(SQ2016ZY03002918),andtheAgriculturalScienceandTechnologyInnovationProgramofCAAS.

Abstract:

DELLA proteins are negative regulators in GA signal pathway. DELLA protein is able to response to various environmental signals and plays an important role in plant resistance to biotic and abiotic stresses. The DELLA gain-of-function mutant Aibian 1 has been widely applied in wheat breeding for dwarfness, but its tolerance to salt is unclear. In this study, Aibian 1 was compared with Chinese Spring, Jing 411, and Aiganzao in salt tolerance using Hoagland’s hydroponic culture with 200 mmol L?1 NaCl. Total chlorophyll content, relative water content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured to evaluate the ability of salt tolerance. Immunoblot assay was employed to assess the DELLA protein abundance in wheat seedlings. After seven-day NaCl treatment, Aibian 1 showed no clear withering leaves, whereas the other three cultivars displayed obviously wilting and yellowing leaves. Among the four cultivars, Aibian 1 had the least loss on chlorophyll content and relative water content, the highest SOD activity, and the lowest levels of MDA content after salt treatment. Immunoblot assay indicated the highest accumulation of DELLA protein in Aibian 1 among the four cultivars. Thus, we consider the high level of DELLA protein is closely related to salt tolerance in Aibian 1.

Key words: WheatDELLAproteins, Salttolerance, Chlorophyllcontent, Superoxidedismutase, Malondialdehyde

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