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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (09): 1261-1271.doi: 10.3724/SP.J.1006.2017.01261

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Cloning, Expression and Functional Analysis of Brassinosteroid Receptor Gene (ZmBRI1) from Zea MaysL.

HAO Ling,ZHANG Yu-Shi,DUAN Liu-Sheng,ZHANG Ming-Cai*,LI Zhao-Hu   

  1. Engineering Research Center of Plant Growth Regulator, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Department of Agronomy, Beijing 100193, China
  • Received:2017-02-23 Revised:2017-05-10 Online:2017-09-12 Published:2017-05-23
  • Contact: Zhang mingcai, E-mail: zmc1214@163.com, Tel: 010-62733049 E-mail:hao_ling2011@163.com
  • Supported by:

    This study was supported by the Introduction of International Advanced Agricultural Science and Technology Program (948 Program, 2011-G19).

Abstract:

Brassinosteroids (BRs) isone of very important plant steroidal hormones that are essential in a wide variety of physiological processes. In this study, an encoding brassinosteroid receptorhomologous gene was cloned by homology cloning from maizeB73 inbred lines, and designated as ZmBRI1. Sequence analysis revealed that the full length of ZmBRI1 was 3369bp, encoding 1122 amino acids. Moreover,ZmBRI1 protein was localized in cell membrane by the protein subcellular localization analysis and a ubiquitously expressed receptor kinase expressed highly in young tissues. The transformation ofZmBRI1 into the Arabidopsis dwarf mutant bri1-5 restored the phenotype, including plant height, leaf morphology and pod size. Compared to bri1-5, Brassinolide (BL) inhibited significantly the root growth of transgenic lines,and Propiconazole(Pcz) inhibited the hypocotyl growth, and the expression levels of DWF4 and CPD were decreased in the transgenic plants. Furthermore, with ABA treatment, overexpression of ZmBRI1 in wild type increased the germination rate and plant growth, and decreased the expression of ABA downstream genes RD29A, RD29B, ABI5,and RAB18 compared to wild type. Therefore, ZmBRI1 was not only involved in plant morphogenesis and BR signal transduction, but also played a pivotal role in response to ABA signal.

Key words: Maize, ZmBRI1, Transgenic plant, Brassinosteroid, ABA

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