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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (10): 1458-1467.doi: 10.3724/SP.J.1006.2017.01458

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation, Expression and Binding Function Analysis of the Transcription Factor GmMYB52 in Soybean

XU Ling**,WANG Yuan-Cong**,HE Xiao-Lan,HUANG Yi-Hong,XU Zhao-Long,SHAO Hong-Bo*,ZHANG Da-Yong*   

  1. Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences / Salt-soil Agricultural Research Center, Nanjing 210014, China
  • Received:2017-02-08 Revised:2017-04-20 Online:2017-10-12 Published:2017-05-11
  • Contact: 张大勇, E-mail: cotton.z@126.com, Tel: 025-84391105; 邵宏波, E-mail: shaohongbochu@126.com E-mail:xuling@jaas.ac.cn

Abstract:

The MYB type transcription factors are involved in plant development and response to abiotic stress. GmMYB52 was significantly up-regulated after salt treatment. In order to gain more information about GmMYB52, GmMYB52 of Williams 82 was cloned by RT-PCR. Bioinformatic analysis showed the CDS of GmMYB52 was 1083bp, encoding 360 amino acid residues. A MYB domain was found in the region of 110 to 160 amino acid residues from N-terminal. Blast results showed that GmMYB52 that highly homologous to are GmMYB62, AtMYBSt1 from Arabidopsis, MtMYB52 from Medicago sativa, OsMYBS3 from Oryza sativa and CcMYB-like protein J from Cajanus Cajan. Quantitative PCR (qPCR) results indicated that the transcription level of GmMYB52 was upregulated under ABA and low temperature stresses, under salt, drought and cold stresses, show a bimodal pattern. GmMYB52 was nearly expressed in all detected tissues, except in pods at maturing stage, and its expression level was relatively higher at seedling or flowering stages than at maturing stage. The transcription level of GmMYB52 was high in stem, leaf, and flower, during seedling and blooming stages, and low in root and pods during maturity stage. Subcellular localization results showed that GmMYB52 was located in the nucleus which is in agreement with the localization characteristics of typical transcription factors. Yeast hybrid assay indicated that GmMYB52 had transcriptional activation functions and could bind to several MYB cis-acting element motifs. In conclusion GmMYB52is a typical 1R-MYB transcription factor, and able to bind MYB cis-acting element motifs. We speculate GmMYB52 is involved in response to the abiotic stress and ABA signal transduction pathway.

Key words: Soybean, GmMYB52, Expression analysis, MYB motif, Transcriptional activation activity

[1] Ambawat S, Sharma P, Yadav N R, Yadav R C. MYB transcription factor genes as regulators for plant responses: an overview. Physiol Mol Biol Plants, 2013, 19: 307–321
[2] Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis. Trends Plant Sci, 2010, 15: 573–581
[3] Paz-Ares J, Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J, 1987, 6: 3553–3558
[4] Agarwal M, Hao Y, Kapoor A,Dong C H, Fujii H, Zheng X, Zhu J K. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. J Biol Chem, 2006, 281: 37636–37645
[5] Seo P J, Park C M. Auxin homeostasis during lateral root development under drought condition. Plant Signal Behav, 2009. 4: 1002–1004
[6] Abe H, Urao T, Ito T, Seki M, Shinozaki K,Yamaguchi-Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell, 2003, 15: 63–78
[7] Li L X, Thompson A, Guo M, Yoshida S, Asami T, Chory J, Yin Y. Arabidopsis MYB30 is a direct target of BES1 and cooperates with BES1 to regulate brassinosteroid-induced gene expression. Plant J, 2009, 58: 275–286
[8] Prabu G. Structure of DNA binding MYB transcription factor protein (ScMYBAS1-3) from sugarcane-threading and Ab initio modelling. J Physiol, 2011, 3: 77–82
[9] Prabu G, Prasad D T. Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones. Plant Cell Rep, 2012, 31: 661–669
[10] Yang A, Dai X, Zhang W H. A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot, 2012: 2541–2556
[11] Liu J, Osbourn A, Ma P. MYB transcription factors as regulators of phenylpropanoid metabolism in plants. Mol Plant, 2015, 8: 689–708
[12] Liao Y, Zou H F, Wang H W, Zhang W K, Ma B, Zhang J S, Chen S Y. Soybean GmMYB76, GmMYB92, and GmMYB177 genes confer stress tolerance in transgenic Arabidopsis plants. Cell Res, 2008, 18: 1047–1460
[13] 杜海, 杨文杰, 刘蕾, 唐晓凤, 吴燕民, 黄玉碧, 唐益雄. 大豆MYB转录因子基因GmMYBJ6和GmMYBJ7的克隆及表达分析. 作物学报, 2008, 34: 1179–1187
Du H, Yang W J, Liu L, Tang X F, Wu Y M, Huang Y B, Tang Y X. Cloning and functional identification of the two MYB transcription factors GmMYBJ6 and GmMYBJ7 in soybean. Acta Agron Sin, 2017, 43: 42–50 (in Chinese with English abstract)
[14] 杨文杰, 吴燕民, 唐益雄. 大豆转录因子基因GmMYBJ7的表达及功能分析. 华北农学报, 2012, 27(6): 24–29
Yang W J, Wu Y M, Tang Y X. Expressing and functional analysis of GmMYBJ7 from soybean. Acta Agric Boreali-Sin, 2012, 27(6): 24–29 (in Chinese with English abstract)
[15] Li X, Li J, Zhai Y, Zhao Y, Zhao X, Zhang H, Su L T, Wang Y, Wang Q. A R2R3-MYB transcription factor, GmMYB12B2, affects the expression levels of flavonoid biosynthesis genes encoding key enzymes in transgenic Arabidopsis plants. Gene, 2013, 532: 72–79
[16] Li X, Wang Y, Yan F, Li J, Zhao Y, Zhao X, Zhai Y, Wang Q. Overexpression of soybean R2R3-MYB transcription factor, GmMYB12B2, and tolerance to UV radiation and salt stress in transgenic Arabidopsis. Genet Mol Res, 2016, 15(2): 1–10
[17] 杨文杰, 杜海, 方芳, 杨婉身, 吴燕民, 唐益雄. 大豆两个MYB转录因子基因的克隆及表达分析. 中国农业科学, 2008, 41: 961–970
Yang W J, Du H, Fang F, Yamg W S, WU Y M, Tang Y X. Cloning and characterization of two new MYB transcription factor genes from soybean. Sci Agric Sin, 2008, 41: 961–970 (in Chinese with English abstract)
[18] 魏麦玲, 裴丽丽, 刘佳明, 闵东红, 陈明, 李连城, 马有志, 徐兆师, 张小红. 大豆MYB转录因子基因GmMYB174的克隆及分子特性分析. 植物遗传资源学报, 2015, 16: 94–99
Wei M L, Pei L L, Liu J M, Min D H, Chen M, Li L C, Ma Y Z, Xu Z S, Zhang X H. Isolation and molecular characteristics analysis of soybean transcription factor gene GmMYB174. J Plant Genet Resour, 2015, 16: 94–99 (in Chinese with English abstract)
[19] 许玲, 卫培培, 张大勇, 徐照龙, 何晓兰, 黄益洪, 马鸿翔, 邵宏波. 大豆转录因子基因GmMYB111的克隆及功能分析. 中国农业科学, 2015, 48: 3079–3089
Xu L, Wei P P, Zhang D Y, Xu Z L, He X L, H Y H, Ma H X, Shao H B. Expression and function analysis of the transcription factor GmMYB111 in soybean. Sci Agric Sin, 2015, 48: 3079–3089 (in Chinese with English abstract)
[20] Takahashi R, Yamagishi N, Yoshikawa N. A MYB transcription factor controls flower color in soybean. J Hered, 2013, 104, 149–153
[21] Feller A, Machemer K, Braun E L, Grotewold E. Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. Plant J, 2011, 66: 94–116
[22] Ali Z, Zhang D Y, Xu Z L, Xu L, Yi J X, He X L, Huang Y H, Liu X Q, Khan A A. Trethowan R M, Ma H X. Uncovering the salt response of soybean by unraveling its wild and cultivated functional genomes using tag sequencing. PLoS One, 2012, 7: e48819
[23] Shelton D, Stranne M, MikkelsenL, Pakseresht N, Welham T, Hiraka H, Tabata S, Sato S, Paquette S, Wang T L, Martin C, Bailey P. Transcription factors of lotus: regulation of isoflavonoid biosynthesis requires coordinated changes in transcription factor activity. Plant Physiol, 2012. 159: 531–547
[24] Stracke R, Stracke R, Ishihara H, Huep G, Barsch A, Mehrtens F, Niehaus K, Weisshaar B, Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling. Plant J, 2007, 50: 660–677
[25] Martin C, Paz-Ares J. MYB transcription factors in plants. Trends Genet, 1997, 13: 67–73
[26] Urao T, Yamaguchi-Shinozaki K, Urao S, Shinozaki K. An Arabidopsis MYB homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. Plant Cell, 1993, 5: 1529–1539
[27] Weston K, Myb proteins in life, death and differentiation. Cur Opin Genet Dev, 1998, 8: 76–81
[28] Tian A G, Wang J, Cui P, Han Y J, Xu H, Cong L J, Huang X G, Wang X L, Jiao Y Z, Wang B J, Wang Y J, Zhang J S, Chen S Y. Characterization of soybean genomic features by analysis of its expressed sequence tags. Theor Appl Genet, 2004, 108: 903–913

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