一个芝麻应用核心种质的DNA分子身份证构建

doi:10.3724/SP.J.1006.2018.01010

Abstract

Abstract:

The establishment and research of sesame applied core collection are significantly effective to facilitate the improvement of breeding techniques and exploration of out-standing genes. A sesame applied core collection, including 131 sesame accessions with 23 application-oriented features such as big seeds, high oil content and high lignan content, was established according to the observation and statistics in four various environments for four years. Based on 32 pairs of SSR primers developed from the sesame genome, we screened out seven pairs of primers, bearing high polymorphism, distinct bands and excellent repetition, using the technique of denatured polyacrylamide gel electrophoresis for detecting SSR The more precise detection technique of capillary electrophoresis with fluorescent SSR markers was utilized simultaneously to analyze the 131 sesame germplasm of applied core collection. Totally 53 polymorphic locations were detected out by using six primer pairs, and the most sensitive primer pair could detect 12 polymorphic locations. Subsequently, the results were shown in the form of numbers combined with English letters. As per the rule that the least primer pairs detect the most sesame germplasm, the combinations of six core primer pairs, namely ZMM1494, ZMM1648, ZMM3037, ZMM2818, ZMM1851, and ZMM1935 effectively distinguishing the 131 sesame germplasm of applied core collection were screened out by ID Analysis 4.0. So that, the simple and easy-applied DNA molecular identifications such as “4A32645(AC017)” were constructed, and the various combinations of primers distinguishing the germplasm within groups were screened out simultaneously. The DNA molecular identifications of character strings, bar code and quick response (QR) codes, easily scanned and recognized by electric gadget, were constructed based on the molecular data of 131 sesame germplasm of applied core collection so as to broaden their application ranges, also provide important technical foundation for the standardization of sesame germplasm and the construction of DNA identification bank of sesame varieties.

Key words: applied core collection of sesame germplasm, SSR, DNA molecular identification

Wen-Juan YANG,Yan-Xin ZHANG,Lin-Hai WANG,Xin WEI,Dong-Hua LI,Yuan GAO,Pan LIU,Xiu-Rong ZHANG. Establishment of DNA Molecular Identification for A Sesame (Sesamum indicum L.) Applied Core Collection[J].Acta Agronomica Sinica, 2018, 44(7): 1010-1020.

Figures/Tables 8

Supplementary Table 1

Information of 32 primer pairs"

SSR引物SSR Primer	连锁群Linkage group	SSR重复基元 SSR Repeat Motif	碱基数目Base No.	上游引物序列 Forward Primer (5°-3°)	下游引物序列 Reverse Primer (5°-3°)
ZMM0987	LG1	(AT)₁₅	30	CTTTGATTGGGCCACCCTA	TGTTTGTTCTTCTTCCCCCA
ZMM2254	LG1	(TA)₇ttgtgtctgtatgtatagatagcatgggagggattgcatgaacaatcatgattcttggaagttttgtgtaggtactgttgatgggcttttatgggat(AC)₇att(TA)8tgtac(AT)₇	163	GCTTCCACCTA GCTCGGTTAT	CCAGCAATCAT GTCTGCTTAAT
ZMM2133	LG2	(AT)₁₃gctttgatataaaaatggggggttttaatttgttaattagagtgggaaaaaggagttagtggaaagtcatcagaaattgcacagatgcgta(TAATT)₃tgtata(AT)₆	150	TTCAATGACTC AACACCCACA	CCATTTAGAAGAGCAAATCATGC
ZMM2313	LG3	(GAGATG)₃gagatagagccatcccatctcaaagtttgttgtacatataatactgagttagtgtaagaaatgagagtgagagtgagatgaaaaaatagt(AGGGG)₃agttgaacttcaccatcaagattttaatgggcatcc(ATCA)₄	175	TCAAAGTGTAC CACAAAACGC	TCTTTCTCTCT CAATCATTTGT TTATG
ZMM2218	LG3	(TAT)₄aagttgaatgtttggtcaactaatgctacttatttagaataacttagcgtaattcatttcattttttactagtaatatatttctactgacaaactac(TTTTA)₃	124	GATGGGGAAA GAGATTGGGT	ATTGAATCGACGTAATTTATCCTT
ZMM1832	LG4	(AT)₁₃	26	ACGATGAGGGCATATTCGAT	CCAATTGTATGGTAATGGGCA
ZMM1935	LG4	(TA)₁₃	26	TGGTACACAATTTGGTGCTTG	GAAGGGGGTCAGGTTTAAATA
ZMM2818	LG5	(TTA)₄tttaatatagtataatttttatgaaaaaatcatttaaaaaattggacctc(AT)₁₅	92	CGTGTGCCCAATATTTGAGTT	TCAACCTCCTCCCTACACAA
ZMM2295	LG6	(TAAT)₃aattaaattta(TAAT)₃aattaaattta(TAAT)₃	58	CATAGCCGTTCGAATCCTGT	AAATTCATGATCCAGGGCAA
ZMM2202	LG6	(ATG)₄aactctttgattttccattttgattgattagtcttcaatgtatgatcaaatttcagaagctgtattgattcatttccgggcagtggaagagttgcat(TCA)₄	121	TCAGGAAGAA AGAATTGCTGC	CAACCCAACC ATCCTGACTC
ZMM1762	LG6	(TA)₁₁gacacacacacgagtagagat(AG)₁₃	69	TGCAAGGACAACCAAAATCA	TGCACTGCATTGTCTCCTTT
ZMM4045	LG7	(AT)₈	16	TGTTTGCTCATTCGATTCCTT	AAAACAACATGGTTTGGCATC
ZMM1851	LG7	(AT)₁₆	32	TGACTCTTTCGATTTGGGCT	CGAAAAATACGGGCGTTACT
ZMM2543	LG8	(AT)₇	14	TTGAGAGGGGCGAAAAACTA	GCACAATCATCGAAAAACCA
ZMM1494	LG8	(TA)₁₇	34	TTTATCAGATACAATGCGGGA	GCCACTACTGGACAGCATGA
ZMM4097	LG8	(TTAA)₃	12	CTGGGTTCATT	GGACCAAATTAATCTTTTTCCCT
ZMM2321	LG9	(TA)₈(GA)₇	30	CAACACCACCAACGCATATC	AGCAACGATTCACGACATTG
ZMM1648	LG9	(GA)₁₉	38	TCCTGAATTCAAACGCATTG	TCCTAAACCCTCTGCACCAC
ZMM2524	LG9	(CT)₁₂gggtgtggctcatttctctaataccctttattttatgccaaaaccccaaccccagatccagccttttctgtatccatcc(CT)₁₂	127	TCACTCCTATGCCAAAACCC	CCTTGAAGAAGCAGTGCAGC
ZMM2494	LG9	(GA)₈	16	GTCCGTCAACTCGATCACCT	TTCAACCAAACCCCATCATT
ZMM3037	LG10	(AT)₁₁(CAC)₄cgaggccgtcgtctgcttcttggtagggtgg(AGC)₅	80	ACACATACGGACAGGCACAG	ATATAGCCAGTTTGGCTGCG
ZMM1550	LG10	(CA)₇(TA)₉	32	GGTAAAATGGGTGTCCGTCA	CCCTAGCCGATGGGTTTAAT
ZMM0689	LG11	(AT)₉	18	CGCTTGAATTAATTGCATCTACC	CCAAGTGAACATAGAAATCTGCC
ZMM1816	LG11	(AT)₈(AG)₁₀	36	CCAGCTCTATTGTGCGTTGA	CACTGCTTTCTCTGAAAGGCT
ZMM2283	LG12	(TTA)₆agtagattataacaacctcttgtaatagataatataattataaaatataaaattagtttgactattaatatgaacacgcatatgagtacctttattgtc(T)₁₈	135	CGCCTTTCTCC TCCTTATCC	CATTCAGTCTTACGTCCAAATTTCT
ZMM2285	LG12	(TA)₈tttataaaa(AG)₆	37	ACTGCACCCTCTGCATTTTT	GCACGTGTGGGGTACCTTTA
ZMM0334	LG12	(AT)₁₄	28	AATTGGACTCCGGCTAGGAT	CGCCCTCATCCTTACAATCT
ZMM0715	LG12	(AT)₁₂	24	CCCCTCTCAAATAAGCCCTC	AGGAAGGAGGGTGTCCCTAA
ZMM0294	LG13	(TCA)₅(CCA)₄	27	GGCCAACCCTTTTCAGATTT	GGGCTTCACAACACAAGACA
ZMM0425	LG13	(AT)₁₅	30	CAAGTCGCCATCACACTCAT	TCGAGTTGGAATGCAACAAA
ZMM1303	LG13	(AT)₁₁	22	TCCCAATCAGTTAGGTCGAG	TTAAGCTTAGGGGTCGGGTT
ZMM2345	LG13	(TA)₁₀	20	CAACATTCTGATTTGGGGAAA	GCTCCAAACCCACTCTGGTA

Supplementary Table 1

Supplementary Table 2

Applied core collection of sesame"

育种目标 Breeding goal	编号 Code	种质名称 Germplasm name	产地或来源 Origin	特性 Property
高产 High yield	AC001	芝麻 Zhima	中国陕西洛川 Luochuan, Shaanxi, China	大粒 Big-sized seed
	AC002	霸王鞭 Bawangbian	中国内蒙古敖汉旗 Aohan banner, Inner Mongolia, China	大粒 Big-sized seed
	AC003	角盘芝麻 Jiaopanzhima	中国山西孝义 Xiaoyi, Shanxi, China	大粒 Big-sized seed
	AC004	一把鞭 Yibabian	中国河南获嘉 Huojia, Henan, China	大粒 Big-sized seed
	AC005	八筒芝麻 Batongzhima	中国河北任邱 Renqiu, Hebei, China	大粒 Big-sized seed
	AC006	芝麻 Zhima	中国辽宁北镇 Beizhen, Liaoning, China	大粒 Big-sized seed
	AC007	灯明寺芝麻 Dengmingsizhima	中国河北东光 Dongguang, Hebei, China	大粒 Big-sized seed
	AC008	小八杈 Xiaobacha	中国河北故城 Gucheng, Hebei, China	大粒 Big-sized seed
	AC009	芝麻 Zhima	中国河北巨鹿 Julu, Hebei, China	大粒 Big-sized seed
	AC010	白芝麻 Baizhima	中国河南济源 Jiyuan, Henan, China	大粒 Big-sized seed
	AC011	叶二三 Ye’ersan	中国河南平舆 Pingyu, Henan, China	大粒 Big-sized seed
	AC012	芝麻 Zhima	中国四川雷波 Leibo, Sichuan, China	大粒 Big-sized seed
	AC013	VIR80	乌兹别克斯坦 Uzbekistan	大粒 Big-sized seed
	AC014	广产 Gungcan	韩国 Republic of Korea	大粒 Big-sized seed
	AC015	U.C.R/82No.16NS	美国 United States	密蒴 Short internode with more capsules
	AC016	犀牛角 Xiniujiao	中国湖北谷城 Gucheng, Hubei, China	长蒴 Long capsule
优质 High quality	AC017	莘东褐芝麻 Shendonghezhima	中国上海市上海县 Shanghaixian, Shanghai, China	高木酚素 High lignan content
	AC018	烈桥黑芝麻 Lieqiaoheizhima	中国江西弋阳 Yiyang, Jiangxi, China	高木酚素 High lignan content
	AC019	S.J	墨西哥 Mexico	高木酚素 High lignan content
	AC020	芝麻 Zhima	中国贵州桐梓 Tongzi, Guizhou, China	高油 High oil content
	AC021	芝麻 Zhima	中国云南楚雄 Chuxiong, Yunnan, China	高油 High oil content
	AC022	EC-351868	印度 India	高油 High oil content
	AC023	L-185	埃及 Egypt	高油 High oil content
	AC024	K2	几内亚 Guinea	高油 High oil content
	AC025	VIR741	乌兹别克斯坦 Uzbekistan	高油 High oil content
	AC026	L161	埃及 Egypt	高油 High oil content
	AC027	白芝麻 Baizhima	中国湖北竹山 Zhushan, Hubei, China	高油 High oil content
	AC028	中芝12 Zhongzhi 12	中国湖北武昌 Wuchang, Hubei, China	高油 High oil content
	AC029	辽品芝2号 Liaopinzhi 2	中国辽宁辽阳 Liaoyang, Liaoning, China	高油 High oil content
	AC030	靖芝1号 Jinzhi 1	中国江西靖安 Jingan, Jiangxi, China	高油 High oil content
	AC031	辽芝2号 Liaozhi 2	中国辽宁阜新 Fuxin, Liaoning, China	高油 High oil content
	AC032	中芝20 Zhongzhi 20	中国湖北武昌 Wuchang, Hubei, China	高油 High oil content
	AC033	白芝麻 Baizhima	中国湖北襄阳 Xiangyang, Hubei, China	高油酸 High acid content
	AC034	绿茎芝麻 Lyujingzhima	中国黑龙江伊春 Yichun, Heilongjiang, China	高油酸 High acid content
	AC035	白芝麻 Baizhima	中国黑龙江依兰 Yilan, Heilongjiang, China	高油酸 High acid content
	AC036	白芝麻 Baizhima	中国贵州晴隆 Qinglong, Guizhou, China	高油酸 High acid content
	AC037	435(40)	日本 Japan	高油酸 High acid content
	AC038	360(36)	日本 Japan	高油酸 High acid content
	AC039	TKV-726(16)	日本 Japan	高油酸 High acid content
	AC040	725(39)	日本 Japan	高油酸 High acid content
	AC041	No. 763	意大利 Italy	高油酸 High acid content
	AC042	白芝麻 Baizhima	中国陕西宝鸡 Baoji, Shaanxi, China	高芝麻素 High sesamin
	AC043	四方芝麻 Sifangzhima	中国湖北通城 Tongcheng, Hubei, China	高芝麻素 High sesamin
	AC044	芝麻 Zhima	中国云南 Yunnan, China	高芝麻素 High sesamin
	AC045	激光2号 Jiguang 2	中国海南海秀油料站 Haixiu, Hainan, China	高芝麻素 High sesamin
	AC046	竹畈芝麻 Zhufanzhima	中国安徽金寨 Jinzhai, Anhui, China	高芝麻素 High sesamin
	AC047	多枝麻 Duozhima	中国重庆云阳 Yunyang, Chongqing, China	高芝麻素 High sesamin
	AC048	黑芝麻 Heizhima	中国安徽肥东 Feidong, Anhui, China	高芝麻素 High sesamin
	AC049	芝麻 Zhima	中国西藏墨脱 Motuo, Tibet, China	高芝麻素 High sesamin
	AC050	双湖芝麻 Shuanghuzhima	中国湖南慈利 Cili, Hunan, China	高芝麻素 High sesamin
	AC051	鄂芝6号 E’zhi 6	中国湖北襄阳 Xiangyang, Hubei, China	高芝麻素 High sesamin
	AC052	中丰芝1号 Zhongfengzhi 1	中国湖北武昌 Wuchang, Hubei, China	高芝麻素 High sesamin
	AC053	Sinyadanar	缅甸 Myanmar	高芝麻素 High sesamin
	AC054	白芝麻 BaiZhima	中国海南琼中 Qiongzhong, Hainan, China	高芝麻素 High sesamin
抗病抗逆Stress and disease resistant	AC055	芝麻 Zhima	中国北京大兴 Daxing, Beijing, China	抗病 Disease resistant
抗病抗逆Stress and disease resistant	AC056	和尚帽 Heshangmao	中国山东金乡 Jinxiang, Shandong, China	抗病 Disease resistant
	AC057	河南1号 Henan 1	中国河南郑州 Zhengzhou, Henan, China	抗病 Disease resistant
	AC058	芝麻 Zhima	中国云南 Yunnan, China	抗病 Disease resistant
	AC059	半汤芝麻 Bantangzhima	中国安徽巢县 Chaoxian, Anhui, China	抗病 Disease resistant
	AC060	黄芝麻 Huangzhima	中国安徽南陵 Nanling, Anhui, China	抗病 Disease resistant
	AC061	341(34)	日本 Japan	抗病 Disease resistant
	AC062	10-2160	中国湖北武昌 Wuchang, Hubei, China	抗病 Disease resistant
	AC063	11-PS56①	中国湖北武昌 Wuchang, Hubei, China	抗病 Disease resistant
	AC064	中芝2771 Zhongzhi 2771	中国湖北武昌 Wuchang, Hubei, China	抗病 Disease resistant
	AC065	长果黄 Changguohuang	中国重庆巫山 Wushan, Chongqing, China	抗倒 Lodging resistance
	AC066	芝麻 Zhima	中国江西德兴 Dexing, Jiangxi, China	抗倒 Lodging resistance
	AC067	白芝麻 Baizhima	中国广东澄海 Chenghai, Guangdong, China	抗倒 Lodging resistance
	AC068	霸王鞭 Bawangbian	中国江苏盱眙 Xuyi, Jiangsu, China	抗倒 Lodging resistance
	AC069	背三歇 Beisanxie	湖北宜昌 Wuchang, Hubei, China	抗倒 Lodging resistance
	AC070	麻芝麻 Mazhima	中国四川宣汉 Xuanhan, Sichuan, China	抗倒 Lodging resistance
	AC071	鄂芝5号 E’zhi 5	中国湖北襄阳 Xiangyang, Hubei, China	抗倒 Lodging resistance
	AC072	98N09	中国河南郑州 Zhengzhou, Henan, China	抗倒 Lodging resistance
	AC073	M20B1	越南 Vietnam	抗倒 Lodging resistance
	AC074	中芝23 Zhongzhi 23	中国 China	抗倒 Lodging resistance
	AC075	Suke No.5(2)	莫桑比克 Mozambique	抗倒 Lodging resistance
	AC076	中芝17 Zhongzhi 17	中国湖北武昌 Wuchang, Hubei, China	抗倒 Lodging resistance
	AC077	豫芝11 Yuzhi 11	中国河南郑州 Zhengzhou, Henan, China	抗倒 Lodging resistance
	AC078	郑芝12 Zhengzhi 12	中国河南郑州 Zhengzhou, Henan, China	抗倒 Lodging resistance
	AC079	驻芝14 Zhuzhi 14	中国河南驻马店 Zhumadian, Henan, China	抗倒 Lodging resistance
	AC080	白芝麻 Baizhima	中国山东曹县 Caoxian, Shandong, China	抗旱 Drought resistant
	AC081	八股杈 Bagucha	中国山东东明 Dongming, Shandong, China	抗旱 Drought resistant
	AC082	白芝麻 Baizhima	中国新疆额敏 Emin, Xinjiang, China	抗旱 Drought resistant
	AC083	白芝麻 Baizhima	中国广东博罗 Boluo, Guangdong, China	抗旱 Drought resistant
	AC084	西关芝麻 Xiguanzhima	中国山西汾阳 Fenyang, Shanxi, China	抗旱 Drought resistant
	AC085	九股钢杈 Jiugugangcha	中国安徽凤阳 Fengyang, Anhui, China	抗旱 Drought resistant
	AC086	芦田黑芝麻 Lutianheizhima	中国江西波阳 Boyang, Jiangxi, China	抗旱 Drought resistant
	AC087	一条鞭 Yitiaobian	中国山东临沂 Linyi, Shandong, China	抗旱 Drought resistant
	AC088	白芝麻 Baizhima	中国广东定安 Dingan, Guangdong, China	抗旱 Drought resistant
	AC089	黑芝麻 Heizhima	中国湖南平江 Pingjiang, Hunan, China	抗旱 Drought resistant
	AC090	VIR214	土耳其 Turkey	抗旱 Drought resistant
	AC091	Potepye	缅甸 Myanmar	抗旱 Drought resistant
	AC092	豫芝1号 Yuzhi 1	中国河南郑州 Zhengzhou, Henan, China	抗旱 Drought resistant
	AC093	汾芝2号 Fenzhi 2	中国山西汾阳 Fenyang, Shanxi, China	抗旱 Drought resistant
	AC094	毛腿糙 Maotuicao	中国河南通许 Tongxu, Henan, China	抗旱 Drought resistant
	AC095	晋芝2号 Jinzhi 2	中国山西汾阳 Fenyang, Shanxi, China	抗旱 Drought resistant
	AC096	芝麻 Zhima	中国贵州遵义 Zunyi, Guizhou	耐湿 Waterlogging tolerant
	AC097	芝麻 Zhima	中国陕西华阴 Huayin, Shaanxi, China	耐湿 Waterlogging tolerant
	AC098	六梭芝麻 Liusuozhima	中国浙江松阳 Songyang, Zhejiang, China	耐湿 Waterlogging tolerant
	AC099	雒荣串麻 Luorongchuanma	中国广西鹿寨 Luzhai, Guangxi, China	耐湿 Waterlogging tolerant
	AC100	鄂芝1号 E’zhi 1	中国湖北襄阳 Xiangyang, Hubei, China	耐湿 Waterlogging tolerant
	AC101	中芝11 Zhongzhi 11	湖北武昌 Wuchang, Hubei, China	耐湿 Waterlogging tolerant
	AC102	U.C.R/82No.209shat	美国 United States	耐湿 Waterlogging tolerant
	AC103	91-2191	以色列 Israel	耐湿 Waterlogging tolerant
	AC104	393-3Bo	泰国 Thailand	耐湿 Waterlogging tolerant
	AC105	Calinda	美国 United States	耐湿 Waterlogging tolerant
	AC106	航芝2号 Hangzhi 2	中国湖北武昌 Wuchang, Hubei, China	耐湿 Waterlogging tolerant
	AC107	中芝18 Zhongzhi 18	中国湖北武昌 Wuchang, Hubei, China	耐湿 Waterlogging tolerant
生育期适宜Suitable growth period	AC108	11-1659	中国湖北武昌 Wuchang, Hubei, China	早熟 Early maturity
适应机械化Mechanization adaptive	AC109	TKV-365(18)	日本 Japan	微裂蒴 Partially capsule dehiscence
适应机械化Mechanization adaptive	AC110	芝麻 Zhima	中国陕西洛川 Luochuan, Shaanxi, China	微裂蒴 Partially capsule dehiscence
	AC111	379(6)	日本 Japan	微裂蒴 Partially capsule dehiscence
	AC112	VDM21	越南 Vietnam	微裂蒴 Partially capsule dehiscence
	AC113	Suke No 5(1)	莫桑比克 Mozambique	有限型 Determinate growth habit
	AC114	有限型No.3 Youxianxing 3	美国 United States	有限型 Determinate growth habit
	AC115	水原117 Suwon 117	韩国 Republic of Korea	有限型 Determinate growth habit
	AC116	7889	中国湖北武昌 Wuchang, Hubei, China	有限型 Determinate growth habit
	AC117	芝麻 Zhima	中国山东加样 Jiayang, Shandong, China	矮杆 Dwarf
	AC118	密蒴芝麻 Mishuozhima	中国浙江东阳 Dongyang, Zhejiang, China	矮杆 Dwarf
	AC119	芝麻 Zhima	中国浙江东阳 Dongyang, Zhejiang, China	矮杆 Dwarf
	AC120	芝麻 Zhima	中国湖北武昌 Wuchang, Hubei, China	矮杆 Dwarf
	AC121	蜂窝油麻 Fengwoyouma	中国浙江东阳 Dongyang, Zhejiang, China	矮杆 Dwarf
株型特异 Specifics of plant morphology	AC122	芝麻 Zhima	中国山西 Shanxi, China	小叶 Small leaves
株型特异 Specifics of plant morphology	AC123	油芝麻 Youzhima	中国安徽肥西 Feixi, Anhui, China	小叶角 Small leaf angles
	AC124	一把白 Yibabai	中国湖北襄阳 Xiangyang, Hubei, China	易再生 Regenerate easily
	AC125	芝麻(8131) Zhima (8131)	中国江西上饶 Shangrao, Jiangxi, China	紧凑型 Compact type
	AC126	庙前芝麻 Miaoqianzhima	中国安徽青阳 Qingyang, Anhui, China	紧凑型 Compact type
综合性状优良Excellent properties overall	AC127	宜阳白 Yiyangbai	中国河南宜阳 Yiyang, Henan, China	耐湿广适应性 Waterlogging tolerant, wide adaptability
综合性状优良Excellent properties overall	AC128	紫花叶二三 Zihuayeersan	中国河南上蔡 Shangcai, Henan, China	抗病抗逆优质 Stress and disease resistant, high quality
	AC129	中芝13 Zhongzhi 13	中国湖北武昌 Wuchang, Hubei, China	高产抗病抗逆高油 High yield, stress and disease resistant, high oil content
	AC130	中芝16 Zhongzhi 16	中国湖北武昌 Wuchang, Hubei, China	高产抗病抗逆高油 High yield, stress and disease resistant, high oil content
	AC131	豫芝4号 Yuzhi 4	中国河南驻马店 Zhumadian, Henan, China	耐湿抗病抗逆广适应性 Waterlogging tolerant, stress and disease resistant, wide adaptability

Supplementary Table 2

Fig. 1

Table 1

Fig. 2

Table 2

Fig. 3

Table 3

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引物名称 Primer name	荧光基团Modify group	毛细管电泳多态性位点 Observed number of alleles (na)	毛细管电泳多态性片段及编码 Code of capillary electrophoresis polymorphism fragments (bp)
ZMM1494	FAM (blue)	12	234 (1), 230 (2), 225 (3), 223 (4), 221 (5), 219 (6), 217 (7), 215 (8), 213 (9), 211 (A), 209 (B), 206 (C)
ZMM1648	FAM (blue)	8	158 (1),156 (2),154 (3),152 (4),150 (5),148 (6),143 (7),139 (8)
ZMM3037	FAM (blue)	8	267 (1), 265 (2), 263 (3), 261 (4), 260 (5), 259 (6), 257 (7), 250 (8)
ZMM1851	FAM (blue)	7	284 (1), 280 (2), 278 (3), 276 (4), 274 (5), 272 (6), 268 (7)
ZMM2818	FAM (blue)	7	278 (1), 276 (2), 274 (3), 272 (4), 268 (5), 264 (6), 260 (7)
ZMM1935	FAM (blue)	6	282 (1), 280 (2), 278 (3), 276 (4), 274 (5), 272(6)
ZMM2254	FAM (blue)	5	282 (1), 280 (2), 278 (3), 276 (4), 274 (5)

Establishment of DNA Molecular Identification for A Sesame (Sesamum indicum L.) Applied Core Collection

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组号 Group No.	特性 Properties	核心引物组合 Core primer combinations
001	大粒 Big-sized seed	ZMM1494+ZMM1851
002	高木酚素 High lignan content	ZMM1494
003	高油 High oil content	ZMM1494+ZMM1851+ZMM3037
004	高油酸 High acid content	ZMM1494+ZMM1935+ZMM2818+ZMM1851
005	高芝麻素 High sesamin	ZMM1494+ZMM3037+ZMM1935
006	抗病 Disease resistance	ZMM1494+ZMM2818
007	抗倒 Lodging resistance	ZMM1494+ZMM1851+ZMM3037+ZMM2818+ZMM1648
008	抗旱 Drought resistance	ZMM1494+ZMM1935+ZMM2818
010	耐湿 Waterlogging tolerant	ZMM1494+ZMM1935
011	微裂蒴 Partially capsule dehiscence	ZMM1494+ZMM1851
015	有限型 Determinate growth habit	ZMM1494+ZMM1935
018	矮秆 Dwarf	ZMM1494
019	紧凑型 Compact type	ZMM2818
022	高产抗病抗逆高油 High yield, stress and disease resistance, high oil content	ZMM1494

[1]	CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[2]	ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[3]	WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[4]	HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
[5]	LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[6]	GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
[7]	WANG Heng-Bo,QI Shu-Ting,CHEN Shu-Qi,GUO Jin-Long,QUE You-Xiong. Development and application of SSR loci in monoploid reference genome of sugarcane cultivar [J]. Acta Agronomica Sinica, 2020, 46(4): 631-642.
[8]	Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[9]	Li-Lan ZHANG, Lie-Mei ZHANG, Huan-Ying NIU, Yi XU, Yu LI, Jian-Min QI, Ai-Fen TAO, Ping-Ping FANG, Li-Wu ZHANG. Correlation between SSR markers and fiber yield related traits in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2020, 46(12): 1905-1913.
[10]	LIU Rong, WANG Fang, FANG Li, YANG Tao, ZHANG Hong-Yan, HUANG Yu-Ning, WANG Dong, JI Yi-Shan, XU Dong-Xu, LI Guan, GUO Rui-Jun, ZONG Xu-Xiao. An integrated high-density SSR genetic linkage map from two F₂ population in Chinese pea [J]. Acta Agronomica Sinica, 2020, 46(10): 1496-1506.
[11]	YE Wei-Jun,CHEN Sheng-Nan,YANG Yong,ZHANG Li-Ya,TIAN Dong-Feng,ZHANG Lei,ZHOU Bin. Development of SSR markers and genetic diversity analysis in mung bean [J]. Acta Agronomica Sinica, 2019, 45(8): 1176-1188.
[12]	Zhi-Jun TONG,Yi-Han ZHANG,Xue-Jun CHEN,Jian-Min ZENG,Dun-Huang FANG,Bing-Guang XIAO. Mapping of quantitative trait loci conferring resistance to brown spot in cigar tobacco cultivar Beinhart1000-1 [J]. Acta Agronomica Sinica, 2019, 45(3): 477-482.
[13]	CHEN Fang,QIAO Lin-Yi,LI Rui,LIU Cheng,LI Xin,GUO Hui-Juan,ZHANG Shu-Wei,CHANG Li-Fang,LI Dong-Fang,YAN Xiao-Tao,REN Yong-Kang,ZHANG Xiao-Jun,CHANG Zhi-Jian. Genetic analysis and chromosomal localization of powdery mildew resistance gene in wheat germplasm CH1357 [J]. Acta Agronomica Sinica, 2019, 45(10): 1503-1510.
[14]	XUE Yan-Tao,LU Ping,SHI Meng-Sha,SUN Hao-Yue,LIU Min-Xuan,WANG Rui-Yun. Genetic diversity and population genetic structure of broomcorn millet accessions in Xinjiang and Gansu [J]. Acta Agronomica Sinica, 2019, 45(10): 1511-1521.
[15]	Jia-Yu YAO,Li-Wu ZHANG,Jie ZHAO,Yi XU,Jian-Min QI,Lie-Mei ZHANG. Evaluation and characteristic analysis of SSRs from the whole genome of jute (Corchorus capsularis) [J]. Acta Agronomica Sinica, 2019, 45(1): 10-17.