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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (6): 867-875.doi: 10.3724/SP.J.1006.2018.00867

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Stability of Trypsin Inhibitor in Foun Bruchid-Resistant Mung Bean Varieties

Yan-Ping FAN1,Xiao-Fang CHENG2,Hong-Min WANG3,Yao-Wen ZHANG4,Xian-Hong ZHANG1,*()   

  1. 1 College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China
    2 College of Arts and Sciences, Shanxi Agricultural University, Taigu 030801, China
    3 College of economics and management, Shanxi Agricultural University, Taigu 030801, Shanxi, China
    4 Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030000, Shanxi, China
  • Received:2017-09-10 Accepted:2018-03-25 Online:2018-06-12 Published:2018-04-16
  • Contact: Xian-Hong ZHANG E-mail:zxh6288@sina.com
  • Supported by:
    The study was supported by the China Agriculture Research System(CARS-08-G10);the Major Project of Key Research and Development Program in Shanxi Province(201703D211002-8)

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Abstract:

Taking bruchid-resistant mung beans C6749, C5200, C5193, and C5205 as experimental materials, with a susceptible mung bean Jinlyu 1 as control, the activity and the stability of mung bean trypsin inhibitor were measured under high temperature, high pressure, denaturant and reductant stresses. The trypsin inhibitor activities of four bruchid-resistant mung beans were significantly higher (P<0.01) than those of control (Jinlyu 1), showing C5200 > C5193 > C6749 > C5205. When treated with high temperature, high pressure, denaturant and reductant, the residual activities of trypsin inhibitor from the four bruchid-resistant mung beans were higher than those of control, and decreased with increasing temperature and pressure, and extending treatment time of denaturant and reductant. The effects of denaturant and reductant on the residual activity of trypsin inhibitor of bruchid-resistant mung beans showed a tendency of guanidine hydrochloride > ure, and TCEP > DTT > β-ME. Among the four bruchid-resistant mung beans, C5200 had the highest tolerance and C5193 had the moderate tolerance to high temperature, high pressure, denaturant and reductant stresses; C5205 had the lowest tolerance to high temperature, high pressure, denaturant stresses, while C6749 had the lowest tolerance to reductant treatment. We concluded that C5200 and C5193 have the highest residual activity of bruchid-resistant mung bean trypsin inhibitor under high temperature, high pressure, denaturant and reductant stresses, being of the higher value in its application.

Key words: bruchid-resistant mung bean, trypsin inhibitor, high temperature and high pressure, denaturant, reductant

Fig. 1

TI activity of different mung bean varieties Bars superscripted by different letters are significantly different at the 0.01 and 0.05 probability levels, respectively."

Table 1

Effect of high temperature and high pressure passivation on TI activity in bruchid-resistant mung bean varieties"

高温高压条件
Condition of high
temperature/high pressure		 绿豆编号
Mung bean code		 残余活性 Residual activity (%)
5 min 10 min 15 min 20 min
0.047 MPa/80°C CK 93.1±1.217 d 90.2±0.361 d 86.2±1.136 d 81.3±0.854 d

C6749 95.2±0.985 c 92.5±0.800 c 90.1±0.557 b 84.1±0.721 c
C5200 99.2±0.557 a 96.3±0.889 a 92.4±0.656 a 88.3±0.794 a
C5193 97.4±1.058 b 94.2±0.819 b 91.2±0.625 ab 86.2±1.136 b
C5205 94.1±0.985 cd 91.1±0.872 cd 88.4±0.755 c 82.8±0.755 cd
均值 Mean 95.80 92.86 89.66 84.54
极差 Range 7.50 7.10 7.60 8.70
变异系数 CV 0.026 0.025 0.026 0.032
高温高压条件
Condition of high
temperature/high pressure		 绿豆编号
Mung bean code		 残余活性 Residual activity (%)
5 min 10 min 15 min 20 min
0.101 MPa/100°C CK 43.7±0.700 e 35.8±0.900 e 24.9±0.755 e 16.7±0.700 e
C6749 59.1±0.721 c 54.1±0.964 c 45.7±0.721 c 30.2±0.755 c
C5200 74.4±0.700 a 70.2±0.800 a 62.5±0.854 a 45.3±0.900 a
C5193 65.2±0.954 b 60.5±0.700 b 54.3±1.249 b 36.4±0.755 b
C5205 50.5±0.800 d 44.1±1.082 d 38.1±0.700 d 24.5±0.755 d
均值 Mean 58.58 52.94 45.10 30.62
极差 Range 32.00 36.10 39.30 30.00
变异系数 CV 0.191 0.236 0.299 0.332
0.143 MPa/110°C CK 35.3±0.608 e 27.7±0.700 e 18.8±0.656 e 13.5±0.800 e
C6749 43.7±0.819 c 39.9±0.872 c 32.7±0.721 c 22.4±0.819 c
C5200 62.1±0.812 a 55.5±0.625 a 47.2±1.136 a 36.2±0.954 a
C5193 50.5±1.114 b 47.2±0.889 b 40.3±0.800 b 28.7±0.700 b
C5205 39.2±0.755 d 34.5±0.755 d 25.9±0.656 d 18.5±0.917 d
均值 Mean 46.16 40.96 32.98 23.86
极差 Range 28.20 29.00 30.30 24.60
变异系数 CV 0.212 0.245 0.317 0.345
0.198 MPa/120°C CK 26.4±0.755 e 18.6±0.600 e 10.2±0.400 e 5.5±0.300 e

C6749 37.4±0.721 c 31.2±0.608 c 22.5±0.700 c 12.4±0.436 c
C5200 53.5±0.800 a 44.7±0.755 a 34.5±0.700 a 25.3±0.458 a
C5193 42.7±0.800 b 35.4±0.755 b 27.4±0.889 b 18.5±0.557 b
C5205 30.6±0.755 d 24.8±0.819 d 17.2±0.755 d 9.5±0.361 d
均值 Mean 38.12 30.94 22.36 14.24
极差 Range 28.60 27.40 25.40 20.60
变异系数 CV 0.259 0.299 0.387 0.507

Table 2

Multi-comparance of TI activity in different mungbean varieties under high temperature and high pressure passivation"

绿豆编号
Mung bean code		 TI活性均值
TI activity mean value		 时间
Time (min)		 TI活性均值
TI activity mean value		 高温高压
High temperature/
high pressure (MPa/°C)		 TI活性均值
TI activity mean value
CK 39.2438 Ee 5 59.6650 Aa 0.047/80 90.7150 Aa
C6749 49.5750 Cc 10 54.4250 Bb 0.101/100 46.8100 Bb
C5200 61.7250 Aa 15 47.5250 Cc 0.143/110 35.9900 Cc
C5193 54.7563 Bb 20 38.3150 Dd 0.198/120 26.4150 Dd
C5205 44.6125 Dd

Table 3

Effect of denaturant on TI activity in bruchid-resistant mung bean"

变性剂
Denaturant		 绿豆编号
Mung bean code		 残余活性 Residual activity (%)
1 h 2 h 3 h
尿素 CK 70.5±0.889 d 30.5±0.625 e 8.4±0.693 e
Urea C6749 80.8±1.758 c 40.3±0.721 c 17.1±0.721 c
C5200 90.5±0.625 a 50.2±0.656 a 30.4±0.964 a
C5193 84.8±1.054 b 44.3±0.625 b 22.3±0.781 b
C5205 80.0±0.700 c 35.1±0.529 d 12.5±1.058 d
均值 Mean 81.32 40.08 18.14
极差 Range 21.20 21.00 23.50
变异系数CV 0.084 0.178 0.440
盐酸胍 CK 33.4±0.656 e 9.4±0.529 e 0.2±0.050 e
Guanidine C6749 42.5±0.755 c 20.3±0.985 c 2.2±0.265 c
hydrochloride C5200 60.4±0.854 a 30.5±0.819 a 5.5±0.500 a
C5193 52.1±0.656 b 26.4±0.721 b 3.8±0.557 b
C5205 39.5±0.781 d 14.5±0.700 d 1.3±0.200 d
均值 Mean 45.58 20.22 2.60
极差 Range 28.40 22.40 5.85
变异系数CV 0.218 0.394 0.753

Table 4

Multi-comparance of TI activity in different mungbean varieties under different denaturant treatments"

绿豆编号
Mung bean
code		 TI活性均值
TI activity
mean value		 时间
Time (min)		 TI活性均值
TI activity
mean value		 变性剂
Denaturant		 TI活性均值
TI activity
mean value
CK 25.4000 Ee 1 63.4500 Aa 尿素 Urea 46.5133 Aa
C6749 33.8667 Cc 2 30.1500 Bb 盐酸胍 Guanidine hydrochloride 22.8000 Bb
C5200 44.5833 Aa 3 10.3700 Cc
C5193 38.9500 Bb
C5205 30.4833 Dd

Table 5

Effect of reductant on TI activity in bruchid-resistant mung bean"

还原剂
Reductant		 绿豆编号
Mung bean code		 残余活性 Residual activity (%)
20 min 40 min 60 min
β-疏基乙醇 CK 61.4±0.781 e 45.3±0.625 e 30.2±0.819 e
β-ME C6749 70.0±1.044 d 55.4±0.700 d 38.5±0.656 d
C5200 90.5±1.389 a 75.6±0.700 a 52.3±0.854 a
C5193 85.7±0.781 b 70.0±0.800 b 45.7±0.700 b
C5205 75.5±0.625 c 59.5±0.819 c 40.2±0.854 c
均值 Mean 76.62 61.16 41.38
极差 Range 30.90 31.50 23.60
变异系数CV 0.142 0.182 0.185
二硫苏糖醇 CK 50.0±0.985 e 34.5±0.755 e 20.5±0.985 e
DTT C6749 57.5±0.755 d 40.0±0.964 d 25.7±1.054 d
C5200 80.5±1.136 a 63.5±0.755 a 43.8±0.854 a
C5193 72.2±0.800 b 58.4±0.700 b 35.2±1.100 b
C5205 61.5±0.800 c 44.3±0.917 c 30.4±0.900 c
均值 Mean 64.34 48.14 31.12
极差 Range 32.10 30.50 24.90
变异系数CV 0.174 0.238 0.267
三(2-羧乙基)膦 CK 42.4±1.044 e 26.5±0.800 e 9.4±0.625 e
盐酸盐 C6749 50.3±1.054 d 33.5±0.854 d 16.2±0.854 d
TCEP C5200 72.3±0.656 a 54.6±0.819 a 35.5±0.854 a
C5193 63.4±0.755 b 49.7±0.964 b 27.6±0.700 b
C5205 56.5±0.755 c 40.2±1.015 c 22.3±0.900 c
均值 Mean 56.98 40.90 22.20
极差 Range 31.30 29.60 27.50
变异系数CV 0.188 0.260 0.421

Table 6

Multi-comparison of TI activity in different mung bean varieties under different reductant treatments"

绿豆编号
Mung bean code		 TI活性均值
TI activity mean value		 时间
Time (min)		 TI活性均值
TI activity mean value		 还原剂
Reductant		 TI活性均值
TI activity mean value
CK 35.5778 Ee 20 65.9800 Aa β-疏基乙醇β-ME 59.7200 Aa
C6749 43.0111 Dd 40 50.0667 Bb 二硫苏糖醇DTT 47.8667 Bb
C5200 63.1778 Aa 60 31.5667 Cc 三(2-羧乙基)膦盐酸盐 TCEP 40.0267 Cc
C5193 56.4333 Bb
C5205 47.8222 Cc
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[1] FAN Yan-Ping,ZHANG Yao-Wen,ZHAO Xue-Ying,ZHANG Xian-Hong. Activity and Physico Chemical Properties of Trypsin Inhibitor in Bruchid-Resistant Mung Bean [J]. Acta Agron Sin, 2017, 43(11): 1696-1704.
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