Fig. 1

Relative expression level of Xig1 in IR24 and W6023 under the inoculation of P6"

Table 1

Cis-acting elements of Xig1IR24 gene promoter region"

Fig. 2

Subcellular localization of XIG1::YFP in rice protoplast YFP: yellow fluorescent signal; Merge: YFP and bright field images were merged."

Fig. 3

Molecular detection of T0 transgenic plants M: marker; WT: IR24 (negative control); 1-32: T0 transgenic plants."

Fig. 4

The target sequences of the 17 homozygous Xig1-edited lines of T2 Capitalized bold letters are PAM sequences; underlined sequences are target sequences; red dashes indicate nucleotide deletions; red lowercase letters indicate inserted nucleotides; italic capital letters are initiation codons. The numbers on the right side of the sequences represent the types of mutation at each target site and the number of nucleotides involved in the mutation. “-” and “+” indicate the number of deletion and insertion nucleotides, respectively. The 100 bp are the bases not shown in this sequence."

Fig. 5

Disease response to Xoo strain P6 of 8 T2 transgenic lines A, C: Disease reactions of 7 days and 14 days after inoculation in eight transgenic lines and IR24. B, D: Lesion length of 7 days and 14 days after inoculation with PXO99A in eight transgenic lines compared with IR24. ** denotes significantly different (P < 0.01). Arrows indicates the inoculated leaves of IR24."

Table 2

Analysis of agronomic traits of 8 gene editing lines"