抗根肿病甘蓝型油菜新品种华油杂62R的选育

doi:10.3724/SP.J.1006.2021.04086

Abstract

Abstract:

The rapeseed clubroot disease incidence in China is about 0.67 million hectare, accounting for 10% of the canola production area, which become a serious threat for the safety of Brassica napus industry. Based on this, we used CR Shinki, a Chinese cabbage material containing CRb clubroot disease resistance locus, as the donor parent, and Pol.CMS restorer line Bing409, the parent of Brassica napus national approved varieties Huayouza 62, as the recipient parent, and the CRb resistance locus was introduced into Bing409 by breeding programs such as crossing, backcrossing, self-cross with the foreground and genetic background selection. In the BC₃F₂ generation, a new restorer line Bing409R with a genetic background close to Bing409 containing CRb resistance locus was obtained, and Huayouza 62R, the first rapeseed hybrid resistant to clubroot disease in China was successfully developed. The results were as follows: CRb disease resistance locus appeared as a dominant single-gene inheritance in B. napus background, and the genetic improvement of resistance to clubroot disease did not at the expense of yield and quality losses for new restorer line Bing409R and its hybrid Huayouza 62R. Bing409R and Huayouza 62R were showed immune-resistance to physiological races of Plasmodiophora brassicae in Sichuan, Hubei, and Anhui provinces in China. This study will provide valuable resources for the breeding of rapeseed in China, and supplemented important support to overcome the threat of rapeseed clubroot disease.

Key words: Brassica napus, clubroot disease, CRb resistance locus, restorer line, genetic improvement, Huayouza 62R

LI Qian, Nadil Shah, ZHOU Yuan-Wei, HOU Zhao-Ke, GONG Jian-Fang, LIU Jue, SHANG Zheng-Wei, ZHANG Lei, ZHAN Zong-Xiang, CHANG Hai-Bin, FU Ting-Dong, PIAO Zhong-Yun, ZHANG Chun-Yu. Breeding of a novel clubroot disease-resistant Brassica napus variety Huayouza 62R[J].Acta Agronomica Sinica, 2021, 47(2): 210-223.

Figures/Tables 15

Table S1

Polymorphic molecular markers used for genetic background screening"

染色体 Chr.	标记名称 Marker name		物理位置 Physical location (Mb)	正向引物 Forward primer (5°-3°)	反向引物 Reverse primer (5°-3°)
A01	niab_ssr113		366,648	CAAAAAGTTGCGGTCAATCT	CCTCCAAAGCTCAATCACTG
	sau_um356		2,137,110	CATCTTCGTCTCTCCATCACCT	GTATGGTAGGAGGAGAGTTCGCT
	cnu_ssr134		4,481,886	TCTCTTTGCCATCGTCGTTTC	CCCCTCAAACTGAGCAGTCAA
	niab_ssr032		7,684,077	TTCTCCCCATCCTCTCATCTTA	ACCCACAACCAACAAAATCTTC
	cnu_mBRPGM0190		9,861,961	GAGATCCAATAGCGAGCACA	TGTGTTATCGGGTGAAGTGG
染色体 Chr.	标记名称 Marker name	物理位置 Physical location (Mb)		正向引物 Forward primer (5°-3°)	反向引物 Reverse primer (5°-3°)
A01	ACMP00456	13,955,674		CTCTACAAGCCGCAGAGAGA	CATCCACCACAGAGATTGCT
	ACMP00617	15,044,996		AACTCAATGCTCTTCGCTCA	CCTTCTGCTGCTTCCAAGAT
	ACMP00297	16,999,515		CAGATATTGCCCAAACACCA	CTTCGCTTCTGAGCTTTCCT
	sau_um586	20,719,752		AGGGTGATGATGATGTACAGGAG	CTTCGTTAATTCTTCCACCCAG
	cnu_ssr125	26,899,174		TCCAAGTGAAGGATAATGCTCGT	TGTACAATGGGGATGTTGTGG
A02	sau-um619	1,404,160		GAGAGCTCACTTGTGCTTCTTGT	CCGATCAAGTTATTCGCTCTCT
	cnu_ssr116	3,025,687		GCCATGGTGGTGGGATTAGA	TGGTCGAACGTGTGTCGATAAT
	niab_ssr143	5,142,784		GATATGTTTCCAAACAAGTCAA	AAATTCGACCCCTTTTCG
	sau_um434	7,390,390		AGCTAGGGATGAGAAGAAGACCG	TCTTTGTGTCCGTCAAGAGTCC
	cnu_ssr036	10,214,155		AGCACAGTCAAAACTCAGAATGA	AGGCATGCGCTCTCTATCTC
	cnu_mBRPGM1391	11,315,073		GGTTTAGAGTGGTGCGGAAT	CCGGGCCTAAAGTAATCAAA
	cnu_mBRPGM0182a	14,588,355		TGGAGTAGAAGCGTCGTCTG	CCTTCCTCCTCTCAGTTCCA
	cnu_mBRPGM1527a	16,911,280		GCAAGCACGGAGACTAAACA	GACGGTGAAGCTGTACGAAG
	cnu_mBRPGM0813	18,824,804		GTTCCATAGGGCGTTCACAT	CCGATGATTCTCTCAATCAGG
	cnu_ssr447	21,870,396		TGGTGTCAAACGGACAGAAA	ATACTCGGCTCAAACCGTCA
	CB10416	23,408,087		GCTGTTGCTGTAGGTTTGA	GAGCCAGCGTTGATAAGA
A03	niab_ssr115	1,049,474		CGGTGTATACCGAACGAGAA	AAACCCAATCAACCCCTTTA
	ACMP00292	2,920,902		GGGTTGCTGGTTTAGCTGTT	TGAATCCGCTGAACTCTCTG
	cnu_mBRPGM0240	5,317,208		GAGGGAAAGAGGACAATACGA	TCATCGAGAAACGAAGGGTA
	cnu_ssr173	8,444,756		TGTATTCCATTATTTCCGACTAACCT	CCGCATTTTAAAAACGTGAGAAA
	cnu_ssr290	9,860,984		CGATTTTGCCATTGTCTAAGC	TGAAGACACGTTGGTTGAACA
	ACMP00755	11,006,524		GTCATCGCAAGAGGACAAGA	AAAGCTCCATCAAAGCACCT
	cnu_ssr098	14,357,780		TGCGACCCAAGTAGGTGAAAC	TGTCTCTCGCTCATTCATCCAA
	cnu_ssr327	17,867,509		TTCTTGACCAAAAGAATCATGG	CTAACACGGGGAAAAGCAGA
	ACMP00186	19,379,932		GCCTCCCTGACTTGTACCTC	TTCAATGCGCCAGTTAAGAC
	ACMP00410	20,585,363		CTCAAAGCATGAACGTGGAC	CCTCCCTTGATTTGTGGAGT
	sau_um146	22,875,333		CTCGCAAAATCCCTTCTTCG	CATATCGCTCGAGTTGCAGA
	cnu_ssr526	23,351,679		TCCGAGAAGCACACAAAGAA	TGACCATTTTCTGCCATTCA
	TCR079	23,692,426		TGACGTTCAATCAAAGCCTGA	TTTAGCAATCAAATGCAAATTCAA
	cnu_ssr492	23,747,774		TCGAGGTGGTTACAATCCAA	CAATGCGGATCTACCTCTCA
	cra_id011	24,345,511		GTCGGATTTCTTTCTACACG	TGAACCTATCTTCCTCAACG
	cra_ssr015	24,345,899		CGGCCTCCGGAAATTTATTA	TGGGAGGACCTCTCTCTTCTT
	Cra_RT1	24,351,487		ACGTAAAGAAGCTGACCGGAGAC	AGGCTTAACAACAGTTCCAGATT
	cra_ssr017	24,355,996		GTGTGACCGCACTGTTGTTT	AGTTTGACCCAAACGCATAA
	sau_um026	25,648,956		AGTGGCTCCCAGGAGGATAATA	CTTGGAGAAGAGAAACTTGGGC
	cnu_ssr241	27,414,883		AATGCTGTGTCCATGACCAA	CGGGCATCCACCTAATTTGT
	At2g35530	29,980,641		CAGAGTAACTGGTTATGCCCGTC	CAATAGGGATAAACCTGGAGACAG
	ACMP00563	31,183,993		CGGAGATGGGATTAAGGAGA	AAAGATCGTGTGGGTGGAAT
A04	BrID10929	251,988		CAATTTGGGAAGACAGTTCT	GATTCGTTGATATAAGGCCA
	BrID10321	2,417,875		TGTGTTTCCTAGTGTGTTGG	ATCAGTCTGAGGGTTCATCA
	BrID101249	3,990,390		TTGCATAGCACATGTAGGAG	GAACGTCTACTTATGGAGAACA
	BrID10645	7,441,204		GCAGAGGAAAACAATCAGAC	AAGCGTCGACTTGAAATCT
	ACMP00073	8,674,696		TTCAACCACACCGACAAACT	CTGACGGAGTCCCTGTACCT
	ACMP00356	11,811,660		TGAGGTCTACAGCCCAAGTG	AATGGAGATCGTGTGCAAAG
染色体 Chr.	标记名称 Marker name	物理位置 Physical location (Mb)		正向引物 Forward primer (5°-3°)	反向引物 Reverse primer (5°-3°)
A04	ACMP00744	12,860,981		CCTGTTCCAATCCCATTCTT	AGATCCCTGACGGGTTTATG
	ACMP00281	15,028,874		TCGACTTTGACGAAGACTGG	AGTCCTCATGGCATCAAACC
	hri_Mbrms195	17,105,559		AATACTTTCTGAAGTTGTCCGCTAA	AACCTACGCAAGATGCTTCTACTT
	cnu_ssr005	18,776,905		AGGAGTCTCGTTCCGTGAGA	TTGAAATTAAGTCGAGCAAACAA
A05	cnu_ssr387	236,456		ACTCGAACCATTCTGGCAAA	GGAACGACTTCCTCCCGTAT
	niab_ssr017	2,501,334		GGTTAAGCAGACGATGGAAGTAA	TATAGGGGAAATCATCTCAAGCA
	cnu_ssr381	4,003,395		TGCTTTTAACCAAACTCAAACG	TGCAGAGAGGCAAGTTTCAA
	sau_um392	5,882,422		GCCAGTTTCGTCTTCTTCTCTG	GAAGTCACACCCCCATCTCTATC
	BrID90500	6,762,400		ATGGGCTTTGTTTGGGTAAA	TGAAAGAGACAAGCTGGCAA
	BrID10825	9,949,084		CAATGTGTTCGATGGAATG	TAGTGTGCGAACAGATTCAG
	ACMP00841	13,132,010		CAGTGGCAACAACATCAACA	CTTCACATCGTCTCCACCAC
	BrID101239	13,226,921		TCCACACAGAAGATAACTGGT	GAGATGACATTTTCGCTGAC
	cnu_ssr458	16,415,511		GGGGTGAATCTTGGATGAGG	CTGACGGATTCCCAACGAAT
	sau_um366	18,549,687		TTCTCCTCGTATCACCACTCCT	GCCTACGTCTTCTACAGCGAGAT
	niab_ssr082	20,079,421		CATTTCCCCGTGACTATCTG	CGTCTTCATCTCAATCTCGC
	sau_um551	21,619,807		GTCCATCTCCTACCTGCTCCA	GTTTTGAGCCGAATAATGGTTG
	hri_mBRMS007	23,268,922		AAATTGTTTCTCTTCCCCAT	GTGTTAGGGAGCTGGAGAAT
A06	BrID10395	1,513,120		GCTGACATGTACCTTTTGAA	CATCTAAGACCGAGTCAAGC
	sau_um278	3,092,702		GAGAACAAGAGGAGGACGCTT	CCGGAGGCAAGTATCCATAAG
	niab_ssr134	5,621,445		CGCAGCCTTTTGCTTCT	TTGCTCTCCTGCAGCTTG
	cnu_mBRPGM1016a	6,689,917		TGGAGATGGCTGTTGTTGAT	AGCAGATGTCGGGAATAACC
	niab_ssr049	7,659,576		GAGGAATTAACGGCGTCTTG	CAGTCGCCACTACCTGGTTT
	ACMP00739	10,965,852		GGTGACTGTTCCTCATCAGC	ATCCCTATCCAAACCTCTCG
	BrID10847	14,198,854		TCATTGCCTTACTTTGTGAC	CTGACACAGGTGAATCAACA
	BrID10849	14,265,494		AAAGATCTGTGGAATCATGG	GGCAAACATGGGTTGATA
	BrID10627	15,630,795		AACACAGATCCAATCTAGCG	GTCCTTAGGCCAAGCATT
	ACMP00692	17,268,545		CGAGTTGCAGAGCCAAGTAG	AACGTAACGCTTCCTCTTCC
	sau-um121	18,555,402		GAACCTAACGAAAGGCACCAC	AGTGAGGGTAGACAGGGAGAGAG
	cnu_ssr220	21,896,695		ATCAGAACCGAATCCGACCA	CAATGGTTGCAATGTTATTTGGA
	sau-um415	22,971,940		ACGCAAAGAGAGCGAAAGAGTC	GGTCTTAATCGCATGGAATCCG
	sau-um616	23,447,329		ATTACCTATTGACCCCACACCAC	GACGTAGAACAAGTGAGAAGGGA
A07	ACMP00261	1,018,274		AAGCCTCGACTTTGGAAGAT	ATCTCCGTCTGGTCTCGTCT
	At2g06510	2,516,307		GATCGGGTTAAGTCAGGACA	ATGGTCTCCATGTTCAGCAC
	ACMP00785	4,675,451		TGGCTCTGTTTCCTTCTCCT	AAGGGATTGATCGGAACTTG
	BrID10283	8,743,728		CGGTTAGGTCGTAACTCGTA	CTCTTCATACGCAAGTCTTT
	BrID10487	11,667,757		TCCCTTACAAGTTCATGGAT	ATGGTAGCAACAAAACCAGT
	nia_m063	12,010,219		GAAGAAACTCGGTGGGGAGT	AAAGAGTTCCGAAAATGGGC
	cnu_ssr511	14,701,383		TGTGGACGAGAAACTGAGGA	TGAGATACTGGTGCGTGTGG
	cnu_ssr044	16,404,302		TGTTTTGATCTTTACTGTTTTTGGA	AATGTTTTTATATCACTATTGCCAAAT
	cnu_ssr048	18,226,238		TTCTCCATGCTGTTCAATTCAC	CATGAAAAATCGACCTTATTCCA
	cnu_ssr516	20,127,600		ACTTGCCTTAGCCAGCAGCG	AAGATTTTGTGTTGTGGTCTGGTGA
	cnu_ssr1566	22,103,544		GTCAGACTCGGATGGCTTG	TAGGAGCAGTTGGTTCAGCA
A08	ACMP00659	1,364,408		CCGCCTCAATCTCTAGCTTT	CTCGTTCACCACCTCTGCTA
	ACMP00551	2,716,097		CAGCAATGGTGGACATCTCT	AACAAGACCGGAACCATCTC
染色体 Chr.	标记名称 Marker name	物理位置 Physical location (Mb)		正向引物 Forward primer (5°-3°)	反向引物 Reverse primer (5°-3°)
A08	BrID90197	4,203,640		TGTTCAAACTTCCCACCCTC	GTGAGCCCAAAAGTTCCTGA
	BrID10839	6,657,012		AGGAACAATACCCATTTGTG	GGTGTTTGTGTGTTCGGTA
	BrID101199	7,356,083		ATGATGGAGATGGACATTTG	TGTATCGGCAGAAGAATCTC
	sau_um192	10,064,084		TCCCTCCTCTCTACGTCTTCTTC	CTTCTCTGTAACGGGCTTTGAC
	sau_um044	14,603,121		CGATTCATCCATCCATCACC	CTATAGGACCGATCCCGTCTTT
	cnu_ssr432	16,431,751		CAAACCTCGTCCTAAGCAGAA	ACCTGAAGATGACCCAGACG
	sau_um077	17,469,120		CTGATCCTCGAAGAAGACAGTGC	CTTCATGCACTTGAGGAGTCG
	cnu_ssr176	18,202,437		TGTAAGTCACGTTCGGTTTGCT	AGGCATGTATGGAGATGTAGAGTGA
	BRMS-198	21,521,373		CGAGAGCAGTTAGGAAGCTTATAGA	AGAGATACTCTGTCCTCCACCTCTT
A09	nia_m010	1,815,031		GGTTGACGTCTCATTGTGTTCTT	TAGCTTTGCTCACTTTTCACTCC
	sau_um219	3,482,038		CGCAGCTTCCTCTGTATTGCTA	GGCTCTCACCAGAGTCAAGTCTA
	cnu_ssr157	5,671,382		CCGCAGTTGATCCATTAGCC	ACGCTGCATCCACATGAAAC
	sau_um368	6,944,590		AGCCCCGTCTCTTTCACTGTAT	GATCTAGGGTCTCGTCGACTTTG
	nia_m022	10,330,441		CTCTCGTCTCGGAGGATCTAAA	GTGAGAGTGGTTGCTGAGTGAG
	sau_um019	13,593,016		GGTCCTGCCATTCCTATTCTCT	CATGCCACGTCAGCAATATG
	sau_um138	16,538,853		CGCACACCATTTCCACAAAC	GAGATGAATGTGCGTCTCCTG
	ACMP00188	18,078,993		GATTCCTCTCCACGACCATT	TCTCCCAAATCGGTTCTTTC
	sau_um101	21,178,401		GATCTTATCGTGCCCATTGC	CTCCTCATAGGGCTCCTTTTTC
	cnu_ssr598	24,440,145		TTCACCGTCTGCTCTTATCG	CTGCTCCCATACGATCCACT
	cnu_ssr016	27,461,366		GGTGAATGGAATCTTGTCTTGA	CCCAACAATCCCAGAAACAC
	cnu_ssr119	30,057,081		ACACCTACTTGTTTCCATCCAAAT	CGGGTATTTGCGTTGTTTCC
	sau_um187	33,166,753		GTCCTCCTCAACCTCATCATCA	AGTCGAGAGTAACGGGAAGAGAG
	sau_um105	36,739,828		CCTTTCTAATGGGAAGCGGTAG	CTCCCTCTTCGAATTGACTCAC
A10	sau_um126	2,528,134		AGAACACGCTCCTAACCATCAG	TAGCTACGAGGCCTTAGAGGGTA
	niab_ssr034	4,065,070		GTGCAAGTCAGTGCCAAAGA	CTCGGTGGTTGAGTGAAGGT
	sau_um433	7,037,138		AAGAGTCCACAGCAGGAGATTG	GGGATGAGAAAAAGACAGGTGG
	sau_um216	10,127,674		CTTTCTTCTCTCCGTCGTTCC	AAGGTTAGGGTTAGAGAAGCCG
	niab_ssr123	11,313,024		GGATCTAGAAACCCCTTCACA	ATCTTGTGTCGGGCAGATAA
	Nniab_ssr122	12,936,001		ACTTCTCCGGCTGGATACTC	CCGTTTAAACTTGCGTTTGT
	niab_ssr009	15,004,910		TTCCCAAGCTTGCTGGTACT	GAGATTTCCCTCGCTTGATG
	sau_um310	15,341,225		TCTTTTCCATCTCTCTACCATCATC	CCTATGAGAGGAAGACCGAGACT

Table S1

Fig. 1

Table 1

Table 2

Table S2

Fig. 2

Fig. 3

Table 3

Fig. 4

Table S3

Table 4

Table 5

Fig. 5

Fig. 6

Table 6

References 24

[1]	Howard R J, Strelkov S E, Harding M W. Clubroot of cruciferous crop-new perspectives on an old disease. Histopathology, 2010,32:35-42. doi: 10.1046/j.1365-2559.1998.00319.x pmid: 9522214
[2]	孙保亚, 沈向群, 郭海峰, 周永红. 十字花科植物根肿病及抗病育种研究进展. 中国蔬菜, 2005, (4):34-37.
	Sun B Y, Shen X Q, Guo H F, Zhou Y H. Research progress in clubroot of crucifers and resistance breeding. China Veget, 2005, (4):34-37 (in Chinese with English abstract).
[3]	Donald C, Porter I. Intergrated control of clubroot. J Plant Growth Regul, 2009,28:289-303. doi: 10.1007/s00344-009-9094-7
[4]	王靖, 黄云, 李小兰, 黎怀忠. 十字花科根肿病研究进展. 植物保护, 2011,37(6):153-158.
	Wang J, Huang Y, Li X L, Li H Z. Research progress in clubroot of crucifers. Plant Protect, 2011,37(6):153-158 (in Chinese with English abstract).
[5]	Diederiehsen E, Frauen M, Linders E G A, Hatakeyama K, Hirai M. Status and perspectives of clubroots resistance breeding in crucifer crops. J Plant Growth Regul, 2009,28:265-281. doi: 10.1007/s00344-009-9100-0
[6]	Yoshikawa H. Studies on breeding of clubroot resistance in cole [Cruciferae] crops. Bull Natl Res Inst Veg Ornam Plants Tea, 1993,A7:1-165.
[7]	Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Fujimura M, Nunome T, Fukuoka H, Matsumoto S, Hirai M. Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in C L. Theor Appl Genet, 2003,107:997-1002. pmid: 12955203
[8]	Sakamoto K, Saito A, Hayashida N, Taguchi G, Matsumoto E. Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Theor Appl Genet, 2008,117:759-767. doi: 10.1007/s00122-008-0817-0 pmid: 18612625
[9]	Piao Z Y, Deng Y Q, Choi S R, Park Y J, Lim Y P. SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp. pekinensis). Theor Appl Genet, 2004,108:1458-1465. doi: 10.1007/s00122-003-1577-5 pmid: 14997298
[10]	Hatakeyama K, Suwabe K, Tomita R N, Kato T, Nunome T, Fukuoka H, Matsumoto S. Identifcation and characterization of Crr1a, a gene for resistance to clubroot disease (Plasmodiophora brassicae Woronin) in Brassica rapa L. PLoS One, 2013,8:e54745. doi: 10.1371/journal.pone.0054745 pmid: 23382954
[11]	Ueno H, Matsumoto E, Aruga D, Kitagawa S, Matsumura H, Hayashida N. Molecular characterization of the CRa gene conferring clubroot resistance in Brassica rapa. Plant Mol Biol, 2012,80:621-629. doi: 10.1007/s11103-012-9971-5 pmid: 23054353
[12]	Hatakeyama K, Niwa T, Kato T, Ohara T, Kakizaki T, Matsumoto S. The tandem repeated organization of NB-LRR genes in the clubroot-resistant CRb locus in Brassica rapa L. Mol Genet Genomics, 2017,292:397-405. doi: 10.1007/s00438-016-1281-1 pmid: 28013378
[13]	Diederichsen E, Beckmann J, Schondelmeier J, Dreyer F. Genetics of clubroot resistance in Brassica napus ‘Mendel’. Acta Hortic, 2006,706:307-312.
[14]	Hwang S F, Strelkov S E, Feng J, Gossen B D, Howard R J. Plasmodiophora brassicae: a review of an emerging pathogen of the Canadian canola (Brassica napus) crop. Mol Plant Pathol, 2012,13:105-113. doi: 10.1111/j.1364-3703.2011.00729.x pmid: 21726396
[15]	符明联, 杨玉珠, 李根泽, 罗延青, 奚俊玉, 徐海燕, 张晓兰, 原小燕, 杨卫国. 不同油菜品种对根肿病的抗性分析. 华中农业大学学报, 2011,30:443-447.
	Fu M L, Yang Y Z, Li G Z, Luo Y Q, Xi J Y, Xu H Y, Zhang X L, Yuan X Y, Yang W G. Analysis of clubroot resistance of different rapeseed varieties. J Huazhong Agric Univ, 2011,30:443-447 (in Chinese with English abstract).
[16]	季海雯, 任莉, 陈坤荣, 徐理, 刘凡, 孙超超, 李俊, 刘胜毅, 方小平. 油菜根肿病病原主要生理小种和品种抗病性鉴定. 中国油料作物学报, 2013,35:301-306.
	Ji H W, Ren L, Chen K R, Xu L, Liu F, Sun C C, Li J, Liu S Y, Fang X P. Identification of physiological races of club root and resistance of rape cultivars to Plasmodiophora brassicae. Chin J Oil Crop Sci, 2013,35:301-306 (in Chinese with English abstract).
[17]	刘勇, 黄小琴, 柯绍英, 刘红雨. 四川主栽油菜品种根肿病抗性研究. 中国油料作物学报, 2009,31:90-93.
	Liu Y, Huang X Q, Ke S Y, Liu H Y. Evaluation of resistance of rapeseed varieties to club root infected by Plasmodiophora brassicae in Sichuan. Chin J Oil Crop Sci, 2009,31:90-93 (in Chinese with English abstract).
[18]	余青青, 田露申, 牛应泽, 郭世星. 人工合成甘蓝型油菜抗根肿病遗传研究初报. 西南农业学报, 2008,21:1313-1315.
	Yu Q Q, Tian L S, Niu Y Z, Guo S H. Preliminary study on inheritance of clubroot resistance in a resythesized Brassica napus line. Southwest China J Agric Sci, 2008,21:1313-1315 (in Chinese with English abstract).
[19]	战宗祥, 江莹芬, 朱紫媛, 张春沙, 杨庆勇, 李倩, 侯照科, 龚建芳, 程雨贵, 吴江生, 傅廷栋, 周永明, 朴钟云, 张椿雨. 与位点PbBa8.1紧密连锁分子标记的开发及甘蓝型油菜根肿病抗性育种. 中国油料作物学报, 2015,37:766-771.
	Zhan Z X, Jiang Y F, Zhu Z Y, Zhang C S, Yang Q Y, Li Q, Hou Z K, Gong J F, Cheng Y G, Wu J S, Fu T D, Zhou Y M, Piao Z Y, Zhang C Y. Development of close linked marker to PbBa8.1 conferring canola resistance to Plasmodiophora brassicae. Chin J Oil Crop Sci, 2015,37:766-771 (in Chinese with English abstract).
[20]	周永红, 孙保亚, 沈向群. 大白菜根肿病抗病接种浓度的研究. 辽宁农业科学, 2007, (3):34-35.
	Zhou Y H, Sun B Y, Shen X Q. Study on inoculation concentration of clubroot resistance in Chinese cabbage. Liaoning Agric Sci, 2007, (3):34-35 (in Chinese with English abstract).
[21]	汪春, 李凯旋, 彭衍彪, 檀根甲, 鲍周明, 方春华. 安徽油菜根肿病菌生理小种鉴定及品种抗病性评价. 安徽农业大学学报, 2014,41:772-776.
	Wang C, Li K X, Peng Y B, Tan G J, Bao Z M, Fang C H. Identification of physiological races of Plasmodiophora brassicae and resistance of rape cultivars to club root in Anhui. J Anhui Agric Univ, 2014,41:772-776 (in Chinese with English abstract).
[22]	杨宗辉. 甘蓝型油菜波里马细胞质雄性不育恢复基因的图位克隆和功能研究. 华中农业大学博士学位论文, 湖北武汉, 2016.
	Yang Z H. Map-based Cloning and Function Analysis of the Restorer Gene of Polima cytoplasmic Male Sterility in Brassica napus. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2016 (in Chinese with English abstract).
[23]	Chai A L, Xie X W, Shi Y X, Li B J. Research status of clubroot (Plasmodiophora brassicae) on cruciferous crops in China. Can J Plant Pathol, 2014,36:142-153.
[24]	Zheng J, Wang X L, Li Q, Yuan S, Wei S Q, Tian X Y, Huang Y, Wang W M, Yang H. Characterization of five molecular markers for pathotype identification of the clubroot pathogen Plasmodiophora brassicae. Phytopathology, 2018,108:1486-1492. doi: 10.1094/PHYTO-11-17-0362-R pmid: 29996697

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标记名称 Marker name	标记类型 Marker type	正向引物 Forward primer (5°-3°)	反向引物 Reverse primer (5°-3°)	物理位置 Physical location (Mb)
CRb_ssr541	SSR	TGCTTGAGCAGAAACAATATCAA	TTGCGCATCTCTGTTTAGCTT	A03: 23693689
CRb_ssr413	SSR	ATTGTGCCGTCGGAATTAAA	GATGATTAGAAAAGGTGTCTATTGC	A03: 23762587
CRb_ssr01	SSR	TCGAGGTGGTTACAATCCAA	CAATGCGGATCTACCTCTCA	A03: 24031219
Rfp_ssr52	SSR	TCAACAACAACAGCCTTTCG	GGAAGAAGTCGCTTCCTGTG	A09: 34418209
Rfp_rt5	功能标记FM	GGGATGCGATCCTGATATTTG	GAGAGAGGCTACAGAACAAACT	A09: 34485601

发病等级 Disease level	调查株数Number of plants investigated
发病等级 Disease level	F₁	BC₁	CR Shinki	Bing409
4级 Level 4	0	19	0	35
3级 Level 3	0	0	0	12
2级 Level 2	0	0	0	0
1级 Level 1	0	0	0	0
0级 Level 0	31	24	30	0

世代 Generation	抗病位点前景选择Foreground selection			背景筛选标记数 No. of markers used to background selection	最终保留株数 No. of selected plants	背景回复率 Genetic background recovery rates (%)	用途 Application
世代 Generation	检测株数 No. of plants tested	筛选标记数 No. of markers	入选株数 No. of selected plants	背景筛选标记数 No. of markers used to background selection	最终保留株数 No. of selected plants	背景回复率 Genetic background recovery rates (%)	用途 Application
BC₁F₁	492	5	256	118	5	64-71	回交Backcross
BC₂F₁	232	5	107	36	4	73-93	回交Backcross
BC₃F₁	267	5	139	5	6	87-97	回交、自交 Backcross, self-cross

根肿菌 P. brassica	收集地点 Collection place	Bing409R接菌株数Number of inoculated Bing409R plants (R/S)			对照 Control (R/S)
根肿菌 P. brassica	收集地点 Collection place	重复1 Repeat 1	重复2 Repeat 2	重复3 Repeat 3	对照 Control (R/S)
Y-TC	云南腾冲Tengchong, Yunnan	15 (3/12)	24 (5/19)	26 (5/21)	19 (0/19)
Y-XC	云南楚雄Xiongchu, Yunnan	19 (16/3)	25 (19/6)	25 (21/4)	25 (0/25)
Y-BS	云南保山Baoshan, Yunnan	24 (5/19)	26 (6/20)	23 (4/19)	20 (0/20)
Y-LC	云南临沧Lincang, Yunnan	20 (11/9)	25 (15/10)	26 (16/10)	21 (0/21)
Y-DH	云南德宏Dehong, Yunnan	20 (4/16)	26 (6/20)	24 (5/19)	25 (0/25)
H-ES	湖北恩施Enshi, Hubei	17 (4/13)	26 (6/20)	20 (3/17)	22 (0/22)
H-YC	湖北宜昌Yichang, Hubei	23 (23/0)	22 (22/0)	26 (26/0)	22 (0/22)
H-ZJ	湖北枝江Zhijiang, Hubei	22 (22/0)	21 (21/0)	19 (19/0)	20 (0/22)
S-DY	四川德阳Deyang, Sichuan	26 (26/0)	22 (22/0)	24 (24/0)	22 (0/22)
S-PZ	四川彭州Pengzhou, Sichuan	27 (27/0)	22 (22/0)	23 (23/0)	20 (0/20)
S-GH	四川广汉Guanghan, Sichuan	25 (25/0)	23 (23/0)	23 (23/0)	25 (0/22)
S-CD	四川成都Chengdu, Sichuan	24 (24/0)	20 (20/0)	19 (19/0)	25 (0/22)
A-HS	安徽黄山Huangshan, Anhui	17 (17/0)	19 (19/0)	23 (23/0)	21 (0/22)

根肿菌编号 No. of P. brassica	收集地点 Collection locations	409R相对病情指数Relative disease index of 409R			抗性评价 Resistance evaluation
根肿菌编号 No. of P. brassica	收集地点 Collection locations	重复1 Repeat 1	重复2 Repeat 2	重复3 Repeat 3	抗性评价 Resistance evaluation
Y-TC	云南腾冲Tengchong, Yunnan	40	40	40	S (40±0)
Y-XC	云南楚雄Xiongchu, Yunnan	8	12	8	R (9±1)
Y-BS	云南保山Baoshan, Yunnan	40	38	41	S (40±1)
Y-LC	云南临沧Lincang, Yunnan	23	20	19	MS (23±1)
Y-DH	云南德宏Dehong, Yunnan	40	38	40	S (39±1)
H-ES	湖北恩施Enshi, Hubei	38	38	43	S (40±1)
H-YC	湖北宜昌Yichang, Hubei	0	0	0	I (0±0)
H-ZJ	湖北枝江Zhijiang, Hubei	0	0	0	I (0±0)
S-DY	四川德阳Deyang, Sichuan	0	0	0	I (0±0)
S-PZ	四川彭州Pengzhou, Sichuan	0	0	0	I (0±0)
S-GH	四川广汉Guanghan, Sichuan	0	0	0	I (0±0)
S-CD	四川成都Chengdu, Sichuan	0	0	0	I (0±0)
A-HS	安徽黄山Huangshan, Anhui	0	0	0	I (0±0)

Breeding of a novel clubroot disease-resistant Brassica napus variety Huayouza 62R

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染色体 Chr.	标记数目Marker numbers	标记间平均间距 Average distance of adjacent markers (Mb)
A01	10	2.95
A02	11	2.30
A03	22	2.01
A04	10	2.07
A05	13	1.92
A06	14	1.83
A07	11	2.11
A08	11	2.02
A09	14	2.69
A10	8	1.83
总数Total	124	—

抗病株系 Resistant lines	含油量 Oil content (%)	芥酸 Erucic acid (%)	硫甙 Glucosinolate (μmol g^-1)
622-22	41.81	0.17	21.76
622-37	40.05	0	24.61
623-30	40.99	0	23.49
624-04	43.63	0.34	26.54
625-17	41.11	0	24.32
630-11	40.65	0	23.33
18ZP06	42.66	0	23.69
18ZP07	42.44	0	30.05
18ZP08	41.99	0.08	28.61
409S01 (CK)	41.06	0	22.76
409S02 (CK)	40.38	0	24.31
409S03 (CK)	43.78	0	23.78

品种 Variety	株高 Plant height (cm)	有效分枝数 Effective branch number	单株有效角果数 Silique number per plant	每角粒数 Seeds per silique	千粒重 Thousand-seed weight (g)	单株产量 Yield per plant (g)	小区产量 Yield per plot (kg)
华油杂12 Huayouza 12	170.8	5.6	162.2	20.9	3.41	11.6	6.367
华油杂62R Huayouza 62R	161.2	5.8	176.8	18.1	3.62	11.6	6.267

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