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作物学报 ›› 2008, Vol. 34 ›› Issue (05): 754-763.doi: 10.3724/SP.J.1006.2008.00754

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中国西部芥菜型油菜遗传多样性研究

徐爱遐1,2;马朝芝2;肖恩时1;权景春1;马长珍1;田广文1;涂金星2,*;傅廷栋2;张改生1   

  1. 1 西北农林科技大学农学院, 陕西杨凌712100; 2 华中农业大学作物遗传改良国家重点实验室, 湖北武汉430070
  • 收稿日期:2007-08-11 修回日期:1900-01-01 出版日期:2008-05-12 发布日期:2008-05-12
  • 通讯作者: 涂金星

Genetic Diversity of Brassica juncea from Western China

XU Ai-Xia12,MA Chao-Zhi2,XIAO En-Shi1,QUAN Jing-Chun1,MA Chang-Zhen1,TIAN Guang-Wen1,TU Jin-Xing2*,FU Ting-Dong2,ZHANG Gai-Sheng1   

  1. 1 College of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi; 2 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
  • Received:2007-08-11 Revised:1900-01-01 Online:2008-05-12 Published:2008-05-12
  • Contact: TU Jin-Xing

摘要: 利用23对SRAP引物、11对AFLP引物和10对SSR引物对我国西部地区的芥菜型油菜及其近缘种108份材料进行了遗传多样性分析, 共检测到313个等位变异, 3种标记的每对引物平均分别可检测到6.8、12.5和1.9个等位变异。包括白菜型和芸芥在内的108份品种间遗传相似系数在0.378~0.936之间, 103份芥菜型油菜品种间遗传相似系数在0.545~0.936之间。聚类分析结果表明, 在相似系数0.558处, 5个参照品种白菜型油菜、小白菜以及芸芥首先被聚出芥菜型油菜之外; 在相似系数0.70处, 103份芥菜型油菜可分为云贵陕南冬播(A)、关中冬播(B)、新疆I (C)、新疆II (D)和西部春播(E)五个类群, 其中A、B基本为冬播品种, C、D、E为春播品种。A类群品种间遗传差异最大, B类群其次。陕西和新疆的品种均分别被聚到3个类群, 表现出更广泛的遗传多样性。春播类型绝大部分被聚到E类群, E类群可分为3个亚类, 其中陕北及其邻近一带春播黄芥为一类, 形成一个独立的遗传群体, 群内遗传多样性较高; 西藏的10个品种为一类, 相似系数高达0.83以上, 表现出西藏品种遗传系统的独立和遗传基础的单一; 澳大利亚2个品种单独为一类, 与我国的春播品种关系较近。由此说明, 地理和生态条件是影响芥菜型油菜类群的主要因素, 我国的冬播品种间的遗传多样性高于春播品种, 陕西和新疆的芥菜型油菜遗传多样性较高。

关键词: 芥菜型油菜, 遗传多样性, SRAP标记, AFLP标记, SSR标记

Abstract: A better understanding of genetic diversity and its distribution are essential for its conservation and use. The research of it will help us to determin what and where to be conserved, and improve our understanding of the taxonomy, origin and evolution of plant species. The genetic diversities of 108 accessions including 101 entries of Brassica juncea from western China, 2 from Australia, 4 entries of B. rapa, and 1 entiry of Eruca sativa Mill.were analysed by SRAP with 23 pairs of primer combinations, AFLP with 11 primer combinations, and SSR with 10 pairs of primer combinations. The results showed that totally 313 loci were detected in these materials. The genetic similarity coefficients of 108 accessions varied from 0.378–0.936, while 103 accessions of B. juncea from 0.545–0.936. The clustering analysis indicated that the genetic similarity coefficients of 5 checks including B. rapa, Eruca sativa Mill., were less than 0.558. At the point of genetic similarity coefficient, 0.700, the 103 accessions of B. juncea were divided into 5 groups, those were group A from Yunnan-Guizhou and Southern Shaanxi, group B from Guanzhong of Shaanxi, group C and group D from Xinjiang, and group E from western China. Groups A and B were winter type, groups C, D, and E were spring type. The genetic difference among the accessions in group A was the largest, and higher than that in group B. The accessions from Shaanxi and Xinjiang were distributed into 3 groups respectively, and showed abundant genetic diversity. Group E including the most spring accessions, was divided into 3 sub-groups. The accessions in the sub-group I were from Tibet, with the genetic similarity coefficient higher than 0.83, belonging to an independent genetic system with narrow genetic background. The accessions of yellow mustard in the sub-group II were from Northern Shaanxi, showed higher genetic diversity and belonged to another independent genetic system. In the sub-group III, two accessions from Australia were similar to the spring type in China. Therefore the genetic differences in B. juncea were mainly related to geological and biological conditions. The genetic diversities in winter type of B. juncea were higher than those in spring type in China. The genetic background of B. juncea in Shaanxi and Xinjiang was wide.

Key words: Brassica juncea, Genetic diversity, SRAP markers, AFLP markes, SSR markers

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