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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (3): 770-777.doi: 10.3724/SP.J.1006.2022.14035

• RESEARCH NOTES • Previous Articles    

Identification and phylogenetic analysis of the pathogen of jute anthracnose in China

GUO Yan-Chun1,2,3**(), YAO Jia-Yu1,2,3**(), ZHANG Rong-Bin1,2, CHEN Si-Yuan1,2,3, HE Qing-Yao1,2,3, TAO Ai-Fen1,2, FANG Ping-Ping1,2, QI Jian-Min1,2, ZHANG Lie-Mei1, ZHANG Li-Wu1,2,3,*()   

  1. 1Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops/Fujian Key Laboratory for Crop Breeding by Design/College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
    2Experiment Station of Ministry of Agriculture and Rural Affairs for Jute and Kenaf in Southeast China/Public Platform of Fujian for Germplasm Resources of Bast Fiber Crops/Fujian International Science and Technology Cooperation Base for Genetics, Breeding and Multiple Utilization Development of Southern Economic Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
    3Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
  • Received:2021-02-26 Accepted:2021-07-12 Online:2022-03-12 Published:2021-08-05
  • Contact: ZHANG Li-Wu E-mail:565358452@qq.com;3533551417@qq.com;lwzhang@fafu.edu.cn;zhang_liwu@hotmail.com
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    National Natural Science Foundation of China(31771369);China Agriculture Research System(CARS-19-E06)

Abstract:

Anthracnose is a major fungal disease that seriously affects the yield and quality in jute fiber. Identifying the types of anthracnose pathogens and determining their pathogenicity will help jute disease-resistant breeding and develop elite disease-resistant gene resources. The 92 strains were isolated and purified from samples of diseased jute, which were collected from 7 jute production areas including Fuzhou and Zhangzhou in Fujian Province, Xinyang City in Henan Province, Liu’an City in Anhui Province, Hangzhou City in Zhejiang Province, Nanning City in Guangxi Province, and Changsha City in Hunan Province. The sequence analysis of the rDNA-ITS region showed that 11 out of the 92 strains were typical anthracnose pathogens. Further identification of the phylogenetic tree of the LSU region and morphological characteristics revealed that 10 strains, including ZZ4 and GX19, were Colletotrichum gloeosporioides, while the strain of CS3 was Colletotrichum dematium. The pathogenicity test of artificial inoculation indicated that there was a significant difference in pathogenicity between Colletotrichum gloeosporioides and Colletotrichum dematium. These results laid a basis for identification of resistance gene loci and effective controlling of anthracnose in jute.

Key words: jute, Colletotrichum, pathogenicity determination, phylogenetic tree

Table 1

Primer information for PCR amplification of rDNA-ITS and LSU in this study"

扩增区域名称
Amplified region name
引物名称
Primer name
引物序列
Primer sequence (5°-3°)
退火温度
Annealing temperature (℃)
rDNA-ITS ITS1 TCCGTAGGTGAACCTGCGG 55
rDNA-ITS ITS4 TCCTCCGCTTATTGATATGC
LSU NL1 GCATATCAATAAGCGGAGGAAAAG 57
LSU NL4 GGTCCGTGTTTCAAGACGG

Fig. 1

Field manifestations of jute disease with anthracnose in different tissues a: symptoms of stems infected with anthracnose; b: symptoms of siliques infected with anthracnose; c: symptoms of leaves infected with anthracnose."

Fig. 2

Phylogenetic tree of rDNA-ITS of 92 strains in jute"

Fig. 3

Phylogenetic tree of LSU of 11 Colletotrichum strains in jute"

Fig. 4

Colonies, conidia, and appressorium morphology of 11 typical Colletotrichum strains in jute a1, b1, c1, d1, e1, f1, g1, h1, i1, j1, and k1 are the obverse sides of colonies of ZZ4, ZZ5, ZZ6, XY13, GX9, GX10, GX17, GX19, CS5, CS13, CS3 Colletotrichum; a2, b2, c2, d2, e2, f2, g2, h2, i2, j2, and k2 are the rear sides of colonies of ZZ4, ZZ5, ZZ6, XY13, GX9, GX10, GX17, GX19, CS5, CS13, CS3 Colletotrichum; a3, b3, c3, d3, e3, f3, g3, h3, i3, j3, and k3 are the conidia of ZZ4, ZZ5, ZZ6, XY13, GX9, GX10, GX17, GX19, CS5, CS13, CS3 Colletotrichum; a4, b4, c4, d4, e4, f4, g4, h4, i4, j4, and k4 are the appressorium of ZZ4, ZZ5, ZZ6, XY13, GX9, GX10, GX17, GX19, CS5, CS13, CS3 Colletotrichum."

Table 2

Resistance performance of Fuhuangma 3 and Funong 5 infected with 11 Colletotrichum strains in jute"

菌株名称
Strain
福黄麻3号病斑平均大小
Average size of Fuhuangma 3 lesions
菌株名称
Strain
福农5号病斑平均大小
Average size of Funong 5 lesions
福州地区Fuzhou region
GX19 11.70 a GX19 16.40 a
XY13 10.30 ab CS13 13.60 ab
CS13 9.80 ab CS5 13.40 abc
CS5 9.70 ab ZZ6 12.80 abc
CS3 9.50 ab ZZ5 11.90 abcd
GX17 9.10 ab GX17 11.70 abcde
GX9 9.00 ab XY13 11.60 abcde
ZZ5 9.00 ab GX10 10.90 bcde
GX10 7.90 ab CS3 8.40 cde
ZZ6 7.80 ab GX9 7.20 de
ZZ4 5.20 b ZZ4 6.80 e
三明地区Sanming region
GX19 13.00 a GX19 13.40 a
GX9 11.10 ab GX9 12.90 a
ZZ5 10.60 bc ZZ5 12.40 a
CS3 10.10 bcd ZZ4 11.50 ab
XY13 9.30 bcde XY13 11.40 ab
GX17 9.20 bcde CS13 10.80 ab
ZZ4 8.70 cde GX17 10.70 ab
ZZ6 8.60 cde ZZ6 10.70 ab
CS13 8.10 def CS3 9.10 bc
CS5 7.30 ef CS5 7.00 c
GX10 6.40 f GX10 6.90 c
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