Welcome to Acta Agronomica Sinica,

Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (3): 770-777.doi: 10.3724/SP.J.1006.2022.14035

• RESEARCH NOTES • Previous Articles    

Identification and phylogenetic analysis of the pathogen of jute anthracnose in China

GUO Yan-Chun1,2,3**(), YAO Jia-Yu1,2,3**(), ZHANG Rong-Bin1,2, CHEN Si-Yuan1,2,3, HE Qing-Yao1,2,3, TAO Ai-Fen1,2, FANG Ping-Ping1,2, QI Jian-Min1,2, ZHANG Lie-Mei1, ZHANG Li-Wu1,2,3,*()   

  1. 1Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops/Fujian Key Laboratory for Crop Breeding by Design/College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
    2Experiment Station of Ministry of Agriculture and Rural Affairs for Jute and Kenaf in Southeast China/Public Platform of Fujian for Germplasm Resources of Bast Fiber Crops/Fujian International Science and Technology Cooperation Base for Genetics, Breeding and Multiple Utilization Development of Southern Economic Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
    3Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.
  • Received:2021-02-26 Accepted:2021-07-12 Online:2022-03-12 Published:2021-08-05
  • Contact: ZHANG Li-Wu E-mail:565358452@qq.com;3533551417@qq.com;lwzhang@fafu.edu.cn;zhang_liwu@hotmail.com
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    National Natural Science Foundation of China(31771369);China Agriculture Research System(CARS-19-E06)


Anthracnose is a major fungal disease that seriously affects the yield and quality in jute fiber. Identifying the types of anthracnose pathogens and determining their pathogenicity will help jute disease-resistant breeding and develop elite disease-resistant gene resources. The 92 strains were isolated and purified from samples of diseased jute, which were collected from 7 jute production areas including Fuzhou and Zhangzhou in Fujian Province, Xinyang City in Henan Province, Liu’an City in Anhui Province, Hangzhou City in Zhejiang Province, Nanning City in Guangxi Province, and Changsha City in Hunan Province. The sequence analysis of the rDNA-ITS region showed that 11 out of the 92 strains were typical anthracnose pathogens. Further identification of the phylogenetic tree of the LSU region and morphological characteristics revealed that 10 strains, including ZZ4 and GX19, were Colletotrichum gloeosporioides, while the strain of CS3 was Colletotrichum dematium. The pathogenicity test of artificial inoculation indicated that there was a significant difference in pathogenicity between Colletotrichum gloeosporioides and Colletotrichum dematium. These results laid a basis for identification of resistance gene loci and effective controlling of anthracnose in jute.

Key words: jute, Colletotrichum, pathogenicity determination, phylogenetic tree

Table 1

Primer information for PCR amplification of rDNA-ITS and LSU in this study"

Amplified region name
Primer name
Primer sequence (5°-3°)
Annealing temperature (℃)

Fig. 1

Field manifestations of jute disease with anthracnose in different tissues a: symptoms of stems infected with anthracnose; b: symptoms of siliques infected with anthracnose; c: symptoms of leaves infected with anthracnose."

Fig. 2

Phylogenetic tree of rDNA-ITS of 92 strains in jute"

Fig. 3

Phylogenetic tree of LSU of 11 Colletotrichum strains in jute"

Fig. 4

Colonies, conidia, and appressorium morphology of 11 typical Colletotrichum strains in jute a1, b1, c1, d1, e1, f1, g1, h1, i1, j1, and k1 are the obverse sides of colonies of ZZ4, ZZ5, ZZ6, XY13, GX9, GX10, GX17, GX19, CS5, CS13, CS3 Colletotrichum; a2, b2, c2, d2, e2, f2, g2, h2, i2, j2, and k2 are the rear sides of colonies of ZZ4, ZZ5, ZZ6, XY13, GX9, GX10, GX17, GX19, CS5, CS13, CS3 Colletotrichum; a3, b3, c3, d3, e3, f3, g3, h3, i3, j3, and k3 are the conidia of ZZ4, ZZ5, ZZ6, XY13, GX9, GX10, GX17, GX19, CS5, CS13, CS3 Colletotrichum; a4, b4, c4, d4, e4, f4, g4, h4, i4, j4, and k4 are the appressorium of ZZ4, ZZ5, ZZ6, XY13, GX9, GX10, GX17, GX19, CS5, CS13, CS3 Colletotrichum."

Table 2

Resistance performance of Fuhuangma 3 and Funong 5 infected with 11 Colletotrichum strains in jute"

Average size of Fuhuangma 3 lesions
Average size of Funong 5 lesions
福州地区Fuzhou region
GX19 11.70 a GX19 16.40 a
XY13 10.30 ab CS13 13.60 ab
CS13 9.80 ab CS5 13.40 abc
CS5 9.70 ab ZZ6 12.80 abc
CS3 9.50 ab ZZ5 11.90 abcd
GX17 9.10 ab GX17 11.70 abcde
GX9 9.00 ab XY13 11.60 abcde
ZZ5 9.00 ab GX10 10.90 bcde
GX10 7.90 ab CS3 8.40 cde
ZZ6 7.80 ab GX9 7.20 de
ZZ4 5.20 b ZZ4 6.80 e
三明地区Sanming region
GX19 13.00 a GX19 13.40 a
GX9 11.10 ab GX9 12.90 a
ZZ5 10.60 bc ZZ5 12.40 a
CS3 10.10 bcd ZZ4 11.50 ab
XY13 9.30 bcde XY13 11.40 ab
GX17 9.20 bcde CS13 10.80 ab
ZZ4 8.70 cde GX17 10.70 ab
ZZ6 8.60 cde ZZ6 10.70 ab
CS13 8.10 def CS3 9.10 bc
CS5 7.30 ef CS5 7.00 c
GX10 6.40 f GX10 6.90 c
[1] Zhang L W, Ibrahim A K, Niyitanga S, Zhang L M, Qi J M. Jute (Corchorus spp.) breeding. In: Al-Khayri J, Jain S, Johnson D, eds. Advances in Plant Breeding Strategies: Industrial and Food Crops. Cham: Springer, 2019. pp 85-113.
[2] 熊和平. 麻类作物育种学. 北京: 中国农业科学技术出版社, 2008. pp 208-296.
Xiong H P. Breeding Sciences of Bast and Leaf Fiber Crops. Beijing: China Agricultural Science and Technology Press, 2008. pp 208-296(in Chinese).
[3] Islam M S, Saito J A, Emdad E M, Ahmed B, Islam M M, Halim A, Hossen Q M, Hossain M Z, Ahmed R, Hossain M S, Kabir S M, Khan M S, Khan M M, Hasan R, Aktar N, Honi U, Islam R, Rashid M M, Wan X, Hou S, Haque T, Azam M S, Moosa M M, Elias S M, Hasan A M, Mahmood N, Shafiuddin M, Shahid S, Shommu N S, Jahan S, Roy S, Chowdhury A, Akhand A I, Nisho G M, Uddin K S, Rabeya T, Hoque S M, Snigdha A R, Mortoza S, Matin S A, Islam M K, Lashkar M Z, Zaman M, Yuryev A, Uddin M K, Rahman M S, Haque M S, Alam M M, Khan H, Alam M. Comparative genomics of two jute species and insight into fibre biogenesis. Nat Plants, 2017, 3:16223.
doi: 10.1038/nplants.2016.223
[4] 祁建民, 李维明, 吴为人. 黄麻的起源与进化研究. 作物学报, 1997, 23:677-682.
Qi J M, Li W M, Wu W R. Research on the origin and evolution of jute. Acta Agron Sin, 1997, 23:677-682 (in Chinese with English abstract).
[5] Rana M, Arora K, Singh S, Singh A K. Corchorus spp.) based on sequence-related amplified polymorphism Corchorus spp.) based on sequence-related amplified polymorphism. J Plant Biochem Biotechnol, 2013, 22:1-18.
doi: 10.1007/s13562-012-0104-7
[6] Niu X P, Gao H, Qi J M, Chen M C, Tao A F, Xu J T, Dai Z G, Su J G. Corchorus capsularis L.) anthracnose in southeastern China Corchorus capsularis L.) anthracnose in southeastern China. Sci Rep, 2016, 6:148-159.
[7] Ikata S, Yoshida M. A new anthracnose of jute plant. Jpn J Phytopathol, 1940, 10:141-149.
doi: 10.3186/jjphytopath.10.141
[8] 刘晓倩, 祁建民, 陈玉森, 陈绵才, 陈美霞, 刘伟, 方平平, 林荔辉, 陶爱芬. 中国红麻炭疽病病原菌的分离鉴定及rDNA-ITS序列分析. 中国农业科学, 2012, 45:3515-3521.
Liu X Q, Qi J M, Chen Y S, Chen M C, Chen M X, Liu W, Fang P P, Lin L H, Tao A F. Identification and rDNA-ITS sequence analysis of the pathogen of kenaf anthracnose. Sci Agric Sin, 2012, 45:3515-3521 (in Chinese with English abstract).
[9] 毕秋艳, 党志红, 朱伟旗, 高占林, 韩秀英, 赵建江, 王文桥, 路粉, 吴杰. 河北省大豆主要病原真菌鉴定及防治药剂筛选. 中国农业科学, 2021, 54:71-85.
Bi Q Y, Dang Z H, Zhu W Q, Gao Z L, Han X Y, Zhao J J, Wang W Q, Lu F, Wu J. Identification of major pathogenic fungi of soybean in hebei province and screening of control fungicides. Sci Agric Sin, 2021, 54:71-85 (in Chinese with English abstract).
[10] 吴家琴, 黎修惕. 黄麻品种资源对黑点炭疽病抗性的鉴定. 中国麻作, 1985, (3):19-25.
Wu J Q, Li X S. Identification of resistance of jute variety resources to Colletotrichum gloeosporiodes. China’s Fiber Crops, 1985, (3):19-25 (in Chinese).
[11] 陈绵才, 吴家琴, 薛召东, 孙家曾, 余隆其. 长果种黄麻种质资源对黑点炭疽病抗性的鉴定. 作物品种资源, 1990, (2):36-37.
Chen M C, Wu J Q, Xue S D, Sun J Z, Yu L Q. Identification of resistance of C. olitorius L. germplasm resources to Colletotrichum gloeosporiodes. Crop Germp Resour, 1990, (2):36-37 (in Chinese).
[12] 张继成, 程新奇, 肖瑞芝, 余方平, 余隆其. 圆果种黄麻种质资源抗炭疽病鉴定研究. 作物品种资源, 1991, (4):34-35.
Zhang J C, Cheng X Q, Xiao R Z, Yu F P, Yu L Q. Study on identification of resistance to anthracnose of C. capsularis L. germplasm resources. Crop Germp Resour 1991, (4):34-35 (in Chinese).
[13] 张镕斌. 中国黄麻主产区炭疽病病原菌分离鉴定及种质资源抗病性评价. 福建农林大学硕士学位论文, 福建福州, 2019.
Zhang R B. Isolation and Identification of the Pathogen of Anthracnose in the Main Jute Producing Areas in China and Evaluation of the Disease Resistance of Germplasm Resources. MS Thesis of Fujian Agriculture and Forestry University, Fuzhou, Fujian, China, 2019 (in Chinese with English abstract).
[14] 高红. 黄麻炭疽病病原菌的分离鉴定及NBS-LRR类抗病基因的克隆. 福建农林大学硕士学位论文,福建福州, 2014.
Gao H. Isolation and Identification of the Pathogen of Jute Anthracnose and Cloning of NBS-LRR Disease Resistance Genes. MS Thesis of Fujian Agriculture and Forestry University, Fuzhou, Fujian, China, 2014 (in Chinese with English abstract).
[15] 林培清, 祁建民, 徐建堂, 方平平, 林荔辉, 陶爱芬, 吴建梅, 池仁漫. 高产抗病圆果黄麻新品种福黄麻3号的选育研究. 中国麻业科学, 2012, 34(3):105-108.
Lin P Q, Qi J M, Xu J T, Fang P P, Lin L H, Tao A F, Wu J M, Chi R M. Selection and breeding of new high yield and disease resistance jute variety Fuhuangma No. 3. Plant Fiber Sci China, 2012, 34(3):105-108 (in Chinese with English abstract)
[16] 彭彩, 蔡敏, 吕发生, 陶洪英, 栾兴茂, 曾晓霞, 李雅玲, 蔡小蓉. 三峡库区菜用黄麻福农5号的品质分析. 中国麻业科学, 2018, 40(4):192-196.
Peng C, Cai M, Lyu F S, Tao H Y, Luan X M, Zeng X L, Li Y L, Cai X R. Quality analysis of vegetable jute Funong No. 5 planted in the three gorges reservoir. Plant Fiber Sci China, 2018, 40(4):192-196 (in Chinese with English abstract).
[17] 郭艳春, 张力岚, 陈思远, 祁建民, 方平平, 陶爱芬, 张列梅, 张立武. 黄麻应用核心种质的DNA分子身份证构建. 作物学报, 2021, 47:80-93.
doi: 10.3724/SP.J.1006.2021.04022
Guo Y C, Zhang L L, Chen S Y, Qi J M, Fang P P, Tao A F, Zhang L M, Zhang L W. Establishment of DNA molecular identification of applied core collection in jute ( Corchorus spp.). Acta Agron Sin, 2021, 47:80-93 (in Chinese with English abstract).
[18] 徐益, 张列梅, 郭艳春, 祁建民, 张力岚, 方平平, 张立武. 黄麻核心种质的遴选. 作物学报, 2019, 45:1672-1681.
doi: 10.3724/SP.J.1006.2019.94008
Xu Y, Zhang L M, Guo Y C, Qi J M, Zhang L L, Fang P P, Zhang L W. Core collection screening of a germplasm population in jute ( Corchorus spp.). Acta Agron Sin, 2019, 45:1672-1681 (in Chinese with English abstract).
[19] 张立武. 基于染色体级别参考基因组和重测序的黄麻重要性状GWAS分析. 见: 2018全国植物生物学大会论文集. 山东泰安, 2018. p 96.
Zhang L W. GWAS analysis of jute important traits based on chromosome-level reference genome and resequencing. In: Proceedings of 2018 National Congress of Plant Biology. Tai’an, Shandong, China, 2018. p 96 (in Chinese).
[20] 徐益, 张力岚, 祁建民, 张列梅, 张立武. 主要麻类作物基因组学与遗传改良: 现状与展望. 作物学报, 2021, 47:997-1019.
doi: 10.3724/SP.J.1006.2021.04121
Xu Y, Zhang L L, Qi J M, Zhang L M, Zhang L W. Genomics and genetic improvement in main bast fiber crops: advances and perspectives. Acta Agron Sin, 2021, 47:997-1019 (in Chinese with English abstract).
[1] YANG Xin, LIN Wen-Zhong, CHEN Si-Yuan, DU Zhen-Guo, LIN Jie, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Li-Wu. Molecular identification of a geminivirus CoYVV and screening of resistant germplasms in jute [J]. Acta Agronomica Sinica, 2022, 48(3): 624-634.
[2] GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
[3] TAO Ai-Fen,YOU Zi-Yi,XU Jian-Tang,LIN Li-Hui,ZHANG Li-Wu,QI Jian-Min,FANG Ping-Ping. Development and verification of CAPS markers based on SNPs from transcriptome of jute (Corchorus L.) [J]. Acta Agronomica Sinica, 2020, 46(7): 987-996.
[4] Li-Lan ZHANG, Lie-Mei ZHANG, Huan-Ying NIU, Yi XU, Yu LI, Jian-Min QI, Ai-Fen TAO, Ping-Ping FANG, Li-Wu ZHANG. Correlation between SSR markers and fiber yield related traits in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2020, 46(12): 1905-1913.
[5] XU Yi,ZHANG Lie-Mei,GUO Yan-Chun,QI Jian-Min,ZHANG Li-Lan,FANG Ping-Ping,ZHANG Li-Wu. Core collection screening of a germplasm population in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2019, 45(11): 1672-1681.
[6] Jia-Yu YAO,Li-Wu ZHANG,Jie ZHAO,Yi XU,Jian-Min QI,Lie-Mei ZHANG. Evaluation and characteristic analysis of SSRs from the whole genome of jute (Corchorus capsularis) [J]. Acta Agronomica Sinica, 2019, 45(1): 10-17.
[7] Yi XU,Lie-Mei ZHANG,Jian-Min QI,Mei SU,Shu-Sheng FANG,Li-Lan ZHANG,Ping-Ping FANG,Li-Wu ZHANG. Correlation Analysis between Yield of Bast Fiber and Main Agronomic Traits in Jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2018, 44(6): 859-866.
[8] ZHANG Yan-Wei,ZHANG Li-Feng,LI Wei,WANG Cai-Jie,ZHANG Jun,XU Ran. Identification, Expression and Variation Analysis of Salt Tolerance Related GmNAC Genes in Soybean [J]. Acta Agron Sin, 2016, 42(07): 990-999.
[9] WU Gui-Fen,XU Xian-Jun,XU Jian-Tang,TAO Ai-Fen,ZHANG Li-Wu,WEI Li-Zhen,PAN Mo,FANG Ping-Ping,LIN Li-Hui,QI Jian-Min. Construction of Molecular Fingerprinting Map in Gene Pool of Jute with SRAP, ISSR and SSR Markers [J]. Acta Agron Sin, 2015, 41(03): 367-377.
[10] ZHANG Li-Wu**,YUAN Min-Hang,HE Xiong-Wei,LIU Xing,FANG Ping-Ping,LIN Li-Hui,TAO Ai-Fen,XU Jian-Tang,QI Jian-Min. Development and Universality Evaluation of EST-SSR Markers in Jute (Corchorus spp.) from GenBank Database [J]. Acta Agron Sin, 2014, 40(07): 1213-1219.
[11] ZHANG Gao-Yang,QI Jian-Min*,XU Jian-Tang,NIU Xiao-Ping,ZHANG Li-Wu,ZHANG Yu-Jia,TAO Ai-Fen,FANG Ping-Ping,LIN Li-Hui. Cloning for Cellulose Synthetase Gene CcCesA1 from Jute (Corchorus capsularis L.), Antisense Vector Construction, and Transformation of Arabidopsis [J]. Acta Agron Sin, 2014, 40(05): 816-822.
[12] DAO Ai-Fen, QI Jian-Min, SU Jian-Guang, LI Ai-Jing, LI Mu-Lan, FANG Beng-Beng, LIN Li-Hui, XU Jian-Tang, TUN Jian-Mei, LIN Pei-Qing. Method Comparison of Using SRAP and ISSR and Combination of Both in Origin and Evolution of Jute [J]. Acta Agron Sin, 2011, 37(12): 2277-2284.
[13] CHEN Fu-Cheng, QI Jian-Min, XU Jian-Tang, CHEN Tao, TAO Ai-Fen, LIN Pei-Qing, CHEN Mei-Xia, GUO Ying, LI Hua-Li. Optimization of Extraction Method and Two-Dimensional Electrophoresis Conditions for Proteome Analysis of Jute Functional Leaf [J]. Acta Agron Sin, 2011, 37(02): 369-373.
[14] WANG Jie-Ming;JIANG Hai-Yang;ZHAO Yang;XIANG Yan;ZHU Su-Wen;CHENG Bei-Jiu. Genome-Wide Analysis of NBS-Encoding disease Resistance Genes in Maize Inbred Line B73 [J]. Acta Agron Sin, 2009, 35(3): 566-570.
[15] DAI Xiao-Jun;OU Li-Jun;LI Wen-Jia;LIANG Man-Zhong;CHEN Liang-Bi. Analysis of rDNA Intergenic Spacer (IGS) Sequences in Oryza sativa L. and Their Phylogenetic Implications [J]. Acta Agron Sin, 2008, 34(09): 1569-1573.
Full text



No Suggested Reading articles found!