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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (07): 990-999.doi: 10.3724/SP.J.1006.2016.00990


Identification, Expression and Variation Analysis of Salt Tolerance Related GmNAC Genes in Soybean

ZHANG Yan-Wei1, 2,ZHANG Li-Feng1,LI Wei1,WANG Cai-Jie1,ZHANG Jun1,XU Ran1, *   

  1. 1 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250131, China; 2 Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin 150001, China
  • Received:2015-12-16 Revised:2016-03-14 Online:2016-07-12 Published:2016-03-28
  • Contact: 徐冉, E-mail: soybeanxu@126.com E-mail:13854198480@163.com
  • Supported by:

    The work was supported by Open Foundation of Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University (SB14A04), China Agriculture Research System (CARS-04-CES18), National Natural Science Foundation of China (31501329), and Shandong Provincial Natural Science Foundation (ZR2015YL070).


NAC genes play an important role in plant stress tolerance. In this study, bioinformatics method was used to identify the stress related GmNAC gene in soybean; the expression of candidated GmNAC genes in root and leaf was analyzed in soybean with NaCl treatment by Real-time-PCR. Reverse transcription PCR was performed to clone genes with significant difference in expression. The results showed that there were 175 genes in soybean GmNAC gene family. There were 11 GmNACproteins with highly conserved NAC located on the same evolutionary branch with the stress related NAC proteins in rice and Arabidopsis. The expression of 11 GmNAC genes in soybean root was higher than that in leaf. The GmNAC genes were all induced by NaCl stress, but part of the GmNAC genes showed different expression levels between root and leaf in soybean varieties with different salt tolerances. There were three synonymous mutations and one non-synonymous mutation on the CDS region of Glyma06g11970.1 and one synonymous mutation on the CDS region of Glyma06g16440.2 in Qihuang 34, Xudou 10, and Fendou 95.

Key words: Soybean, GmNAC, Phylogenetic tree, NaCl treatment, Expression analysis, Sequence variation

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