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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (03): 367-377.doi: 10.3724/SP.J.1006.2015.00367


Construction of Molecular Fingerprinting Map in Gene Pool of Jute with SRAP, ISSR and SSR Markers

WU Gui-Fen1,2,XU Xian-Jun3,XU Jian-Tang1,TAO Ai-Fen1,ZHANG Li-Wu1,WEI Li-Zhen4,PAN Mo5,FANG Ping-Ping1,LIN Li-Hui1,QI Jian-Min1,*   

  1. 1 Key Laboratory of Ministry for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Province International Cooperation Base for Genetics, Breeding and Multiple Utilization of Characteristic Economy Crops in South China , Fuzhou 350002, China; 2 Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3 Wuyishan Biological Resource Institute of Fujian Province, Fuzhou 354300, China; 4 Third Middle School of Fuzhou, Fuzhou 350002, China; 5 Fuzhou Tanditongren Mdt InfoTech Ltd, Fuzhou 350001, China
  • Received:2014-09-14 Revised:2014-12-19 Online:2015-03-12 Published:2015-01-12
  • Contact: 祁建民, E-mail: qijm863@163.com, Tel: 0591-87644898 E-mail:2609267430@qq.com


An experiment was conducted using 231 jute germplasm resources from abroad and at home to construct DNA fingerprints of jute varieties with SRAP, ISSR, SSR marker and the DNA fingerprint analysis software. The results showed that 96 DNA fingerprints from 231 jute germplasm resources with 35 pairs of selected SRAP primers, 45 DNA fingerprints from 96 jute varieties with 11 selected polymorphic ISSR primers, and 13 DNA fingerprints from 48 jute varieties with 49 selected polymorphic SSR primers were constructed. This study completed a total of 154 genomic DNA molecular fingerprint maps of jute varieties. Every identified jute variety had its unique “ID”. Other 77 local varieties had not been able to be identified due to their high genetic similarity with some varieties. It showed that jute local variety has a serious phenomenon of synonym.

Key words: Jute, Genetic resources, Molecular markers, DNA fingerprint

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