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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (04): 530-548.doi: 10.3724/SP.J.1006.2017.00530

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Genetic Diversity in Common Millet (Panicum miliaceum) Using Fluorescent SSR in China

WANG Rui-Yun1,2,**,JI Xu1,**,LU Ping3,LIU Min-Xuan3,XU Yue4,WANG Lun2,WANG Hai-Gang2,QIAO Zhi-Jun2,*   

  1. 1 College of Agriculture, Shanxi Agricultural University, Taigu 030801, China; 2 Institute of Crop Germplasms Resources of Shanxi Academy of Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, China; 3 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4 School of Life Sciences, Jilin University, Changchun 130012, China
  • Received:2016-08-12 Revised:2016-11-02 Online:2017-04-12 Published:2016-11-15
  • Contact: 王瑞云, E-mail: wry925@126.com, Tel:15234420135; 乔治军, E-mail: nkypzs@126.com, Tel: 0351-7065530 E-mail:wry925@126.com
  • Supported by:

    1 College of Agriculture, Shanxi Agricultural University, Taigu 030801, China; 2 Institute of Crop Germplasms Resources of Shanxi Academy of Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, China; 3 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4 School of Life Sciences, Jilin University, Changchun 130012, China

Abstract:

Evaluating the genetic diversity of germplasm resources in common millet is helpful to facilitate the understanding of its origin and evolution, and facilitate to explore the elite germplasm for highly effective utilization. Genetic diversity among 132 accessions of common millet from 11 provinces in China was detected by 15 millet-specific fluorescent-labelled simple sequence repeat (SSR) primers. A total of 107 alleles were detected, with alleles per locus ranging from 2 to 14 (mean = 7). Polymorphism information content and expected heterozygosity ranged from 0.0893 to 0.8538 (mean = 0.4864) and from 0.0936 to 0.8676 (mean=0.5298), respectively. Cluster analysis based on genetic distance separated the accessions into four groups, including Group I from Northeast spring-sowing ecotope, Group II from Loess Plateau spring & summer-sowing ecotope, Group III from North spring-sowing ecotope, and Group IV from North spring-sowing ecotope and Loess Plateau spring & summer-sowing ecotope. Model-based genetic structure analysis indicated that common millet accessions from China were derived from four (Northeast, Loess Plateau, North and Northwest) gene pools. The above two cluster analyses uncovered a close correlation between geographical regions and genetic diversity. There were abundant genetic variations from different accessions of common millet. The obtained information would provide an accurate estimation of the genetic diversity of common millet in China at molecular level.

Key words: Common millet (Panicum miliaceum L.), Fluorescent SSR, Genetic diversity, Cluster analysis, Genetic structure

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