欢迎访问作物学报,今天是

作物学报 ›› 2008, Vol. 34 ›› Issue (11): 1958-1963.doi: 10.3724/SP.J.1006.2008.01958

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

烟草SRAP和ISSR分子遗传连锁图谱构建

马红勃1;祁建民1,*;李延坤1;梁景霞1;王涛2;兰涛1;陈顺辉3;陶爱芬1;林荔辉1;吴建梅1   

  1. 1 福建农林大学作物科学学院/生命科学学院,福建福州350002;2 福建省顺昌烟草公司,福建顺昌353200;3福建省烟草公司,福建福州350003
  • 收稿日期:2008-03-23 修回日期:1900-01-01 出版日期:2008-11-13 网络出版日期:2008-08-12
  • 通讯作者: 祁建民
  • 基金资助:

    国家烟草专卖局资助项目(国烟科[2005])

Construction of A Molecular Genetic Linkage Map of Tobacco Based on SRAP and ISSR Markers

MA Hong-Bo1,QI Jian-Min1*,LI Yan-Kun1,LIANG Jing-Xia1,WANG Tao2,LAN Tao1,CHEN Shun-Hui3,TAO Ai-Fen1,LIN Li-Hui1,WU Jian-Mei1   

  1. 1College of Crop or Life science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian; 2Fujian Shunchang Tobacco Company, Shunchang 353200, Fujian; 3Fujian Tobacco Company, Fuzhou 350003, Fujian, China
  • Received:2008-03-23 Revised:1900-01-01 Published:2008-11-13 Published online:2008-08-12
  • Contact: QI Jian-Min

摘要:

采用烤烟品种台烟7号与白肋烟品种白肋21杂交,构建了187个单株的F2遗传作图群体,利用所筛选的68个能扩增出多态性条带的SRAP和ISSR引物,对F2作图群体进行PCR扩增和遗传连锁分析,初步构建了一张包含26个连锁群、112个(92个SRAP和20个ISSR)标记位点的烟草遗传连锁图谱。该图谱覆盖长度为1 560.2 cM,平均图距18.1 cM。有16个标记未进入连锁群。26个连锁群包含2~20标记不等,连锁群遗传距离0~291.0 cM。连锁群上有24.1%的标记出现偏分离,主要集中在LG1和LG4连锁群上,其余分散在不同连锁群。该图谱为烟草重要农艺性状的基因定位、以及分子标记辅助选择等研究奠定基础。

关键词: 烟草, SRAP, ISSR, 遗传连锁图谱

Abstract:

High-density genetic linkage maps are useful tools for studying genome, phylogeny and evolution, and for mapping and Cloning genes of interest. In this study, a molecular genetic map was constructed with F2 population consisting of 187 individuals from a cross of flue-cured tobacco cultivar Taiyan 7 and burley tobacco cultivar Bailei 21 by using a total of 513 SRAP primers and 42 ISSR primers. Approximately 58 out of 513 and 10 out of 42 primers, respectively, showed polymorphisms. Each of this polymorphic primers produced at least one scorable polymorphic DNA band which was visible enough for detection and scoring. The map consisted of 26 linkage groups which included 112 (92 SRAP and 10 ISSR) markers, and covered 1 560.2 cM with an average distance of 18.1 cM. Sixteen markers were still unlinked. All 26 linkage groups consisted of 2–20 markers ranging in length from 0–291.0 cM. A total of 24.1% distorted markers distributed in the map, mainly concentrated in the LG1 and LG4 linkage groups, and the others were mapped on diverse linkage groups respectively. This map will provide the basis for gene mapping and marker-assisted selection of important agronomic traits in tobacco.

Key words: Tobacco, SRAP, ISSR, Genetic linkage map

[1] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状[J]. 作物学报, 2021, 47(4): 587-598.
[2] 李鹏, 刘彻, 宋皓, 姚盼盼, 苏沛霖, 魏跃伟, 杨永霞, 李青常. 烟草非特异性脂质转移蛋白基因家族的鉴定与分析[J]. 作物学报, 2021, 47(11): 2184-2198.
[3] 董庆园,马德清,杨学,刘勇,黄昌军,袁诚,方敦煌,于海芹,童治军,沈俊儒,许银莲,罗美中,李永平,曾建敏. 高抗黑胫病烤烟BAC文库的构建及分析[J]. 作物学报, 2020, 46(6): 869-877.
[4] 衡友强,游西龙,王艳. 费尔干猪毛菜病程相关蛋白SfPR1a基因的异源表达增强了烟草对干旱、盐及叶斑病的抗性[J]. 作物学报, 2020, 46(4): 503-512.
[5] 陈杉彬, 孙思凡, 聂楠, 杜冰, 何绍贞, 刘庆昌, 翟红. 甘薯IbCAF1基因的克隆及耐盐性、抗旱性鉴定[J]. 作物学报, 2020, 46(12): 1862-1869.
[6] 刘荣, 王芳, 方俐, 杨涛, 张红岩, 黄宇宁, 王栋, 季一山, 徐东旭, 李冠, 郭瑞军, 宗绪晓. 利用2个F2群体整合中国豌豆高密度SSR遗传连锁图谱[J]. 作物学报, 2020, 46(10): 1496-1506.
[7] 马晓寒,张杰,张环纬,陈彪,温心怡,许自成. 通过外源MeJA抑制H2O2积累提高烟草的耐冷性[J]. 作物学报, 2019, 45(3): 411-418.
[8] 童治军,张谊寒,陈学军,曾建敏,方敦煌,肖炳光. 雪茄烟品种Beinhart1000-1赤星病抗性基因的QTL定位[J]. 作物学报, 2019, 45(3): 477-482.
[9] 谈欢,刘玉汇,李丽霞,王丽,李元铭,张俊莲. 马铃薯块茎花色素苷合成相关R2R3 MYB蛋白基因的克隆和功能
分析
[J]. 作物学报, 2018, 44(7): 1021-1031.
[10] 王建伟,贺晓岚,李文旭,陈新宏. 小麦近缘属植物1-FFT基因的克隆及功能分析[J]. 作物学报, 2018, 44(6): 814-823.
[11] 钟思荣,陈仁霄,陶瑶,龚丝雨,何宽信,张启明,张世川,刘齐元. 耐低氮烟草基因型的筛选及氮效率分析[J]. 作物学报, 2017, 43(07): 993-1002.
[12] 赵佩,腾丽杰,王轲,杜丽璞,任贤,佘茂云,叶兴国. 小麦TaVIP1家族基因克隆、分子特性及功能分析[J]. 作物学报, 2017, 43(02): 201-209.
[13] 姚新转,刘洋,赵德刚. 高粱Na+转运蛋白基因SbSKC1的克隆及其在烟草中的抗盐功能鉴定[J]. 作物学报, 2017, 43(02): 190-200.
[14] 吕品,于海峰,于志贤,张永虎,张艳芳,王婷婷,侯建华. 向日葵高密度遗传连锁图谱构建及两种水分条件下芽期性状的QTL分析[J]. 作物学报, 2017, 43(01): 19-30.
[15] 陶瑶,王瑜,钟思荣,吴凌敏,谢丽娟,聂亚平,周玮,王建革,刘齐元. 烟草ATP合酶F0部分4个亚基基因转录本编辑位点分析[J]. 作物学报, 2016, 42(12): 1743-1753.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!