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作物学报 ›› 2009, Vol. 35 ›› Issue (4): 615-619.doi: 10.3724/SP.J.1006.2009.00615

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

阿夫及其衍生小麦品种（系）的SSR分析

司清林12，刘新伦1，刘智奎3，王长有1，吉万全1*   

  1. 1西北农林科技大学农学院，陕西杨凌712100；2济源市农业科学研究所，河南济源454652；3陕西省西安市种子管理站，陕西西安710054
  • 收稿日期:2008-09-22 修回日期:2008-10-15 出版日期:2009-04-12 网络出版日期:2009-02-13
  • 通讯作者: 吉万全
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2006CB101701-9）资助。

SSR Analysis of Funo Wheat and Its Derivatives

SI Qing-Lin12,LIU Xin-Lun1,LIU Zhi-Kui3,WANG Chang-You1,JI Wan-Quan1*   

  1. 1College of Agronomy,Northwest A&F University,Yangling 712100,China;2Institute of Jiyuan Agricultual Science, JiYuan 454652,China;3Seed Management Station of Xi'an City,Xi'an 710054,China
  • Received:2008-09-22 Revised:2008-10-15 Published:2009-04-12 Published online:2009-02-13
  • Contact: JI Wan-Quan

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1958

被引次数

28

摘要：

为了研究小麦骨干亲本阿夫(Funo)遗传物质在其衍生品种(系)的传递规律，用247对SSR引物对阿夫和8个阿夫子一代衍生品种(系)的亲本进行分析，发现有3个标记Xwmc398 (178 bp, 151 bp)、Xgwm400(180 bp, 149 bp)和Xgwm268(191 bp)在阿夫上有稳定、清晰的特异带。以筛选的特异引物对5个阿夫系选品种和255个阿夫衍生品种(系)进行了SSR分析。结果表明，Xwmc398在5个系选品种中均有阿夫的特异带，而Xgwm400只在安选2号，Xgwm268只在扬麦1号具有阿夫特异带。Xwmc398在阿夫子一代至子六代衍生品种(系)中的遗传频率分别为52.8%、38.4%、16.7%、0.0%、0.0%和0.0%, 平均为32.2%；Xgwm400的相应遗传频率分别为32.1%、19.2%、41.7%、33.3%、20.0%和0.0%，平均为26.7%；Xgwm268的相应传递率分别为22.6%、34.4%、11.1%、12.1%、0.0%和0.0%，平均为24.7%。表明SSR位点Xwmc398、Xgwm400和Xgwm268在阿夫衍生品种(系)中有明显的传递。

关键词: 阿夫（Funo）衍生品种, 骨干亲本, SSR特异带

Abstract:

Funo is one of the most important backbone parents in China, and many wheat (Triticum aestivum L.) varieties are derived from it. Funo and 8 parents of the first generation of Funo were analyzed using 247 simple sequence repeat (SSR) markers that covered the entire wheat genome to disclose the inheritance of genes in Funo on genome level and provide theoretical basis on development of backbone parent in wheat. Specific DNA fragments were detected using three SSR markers, Xwmc398, Xgwm400, and Xgwm268. The specific fragments were 178 and 151 bp for Xwmc398, 180 and 149 bp for Xgwm400, and 191 bp for Xgwm268. A total of 255 derivatives of Funo including 54, 124, 36, 33, 5, and 3 varieties respectively from the first to the sixth generations and 5 selected strains of Funo were analyzed using the specific primers. The specific bands of Xwmc398 were observed in all the five selected strains of Funo, whereas, only in Anxuan 2 for Xgwm400 and in Yangmai 1 for Xgwm268,. In the 255 varieties derived from Funo, the inheritable rates of specific bands for Xwmc398 were 52.8%, 38.4%, 16.7%, 0.0%, 0.0%, and 0.0% from the first to the sixth generations, respectively, with an average rate of 32.2%. For Xgwm400, the rates were 32.1%, 19.2%, 41.7%, 33.3%, 20.0%, and 0.0%, with an average of 26.7%. For Xgwm268, the rates were 22.6, 34.4, 11.1, 12.1, 0.0, and 0.0%, respectively, with an average of 24.7%. Xwmc398 had the highest inheritable rate in the Funo-derivative varieties and the most stable inheritance in Funo-selective varieties.

Key words: Funo pedigree, Backbone parent, Specific band of SSR marker

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