欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (8): 1458-1461.doi: 10.3724/SP.J.1006.2009.01458

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

EST辅助的甘蓝型油菜显性核不育AFLP标记转化

宋来强1,2,易斌2,杨明贵2,陈伦林1,傅廷栋2   

  1. 1江西省农业科学院油料作物重点实验室，江西南昌330200；2华中农业大学植物遗传改良国家重点实验室，高家油菜品种改良武汉分中心，湖北武汉430070
  • 收稿日期:2008-12-17 修回日期:2009-03-08 出版日期:2009-08-12 网络出版日期:2009-05-19
  • 作者简介:songlaiqiang@yahoo.com.cn
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2001CB10807），引进国际先进农业科学技术计划（948计划）项目（2006-G04），高家油菜现代产业技术体系（nycytx-00512）项目资助。

EST-Aided Conversion of AFLP Marker Linked to Dominant Male Sterility Gene in Brassica napus into SCAR

SONG Lai-Qiang1,2,YI Bin2,YANG Ming-Gui2,CHEN Lun-Lin1,FU Ting-Dong2   

  1. 1Key Laboratory of Oil Crops，jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;2National Key Laboratory of Crop Genetic Improvement,National Subcenter of Rapeseed Improvement in Wuhan,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2008-12-17 Revised:2009-03-08 Published:2009-08-12 Published online:2009-05-19
  • About author:songlaiqiang@yahoo.com.cn

PDF

1720

被引次数

13

摘要：

甘蓝型油菜显性核不育广泛应用于轮回选择和杂种优势利用，不育基因标记的开发与应用对于基因克隆和育种实践具有重要意义。基于AFLP标记SA12MG14的序列信息，从拟南芥整合数据库中，检索与标记序列同源的甘蓝型油菜EST，结合标记和EST序列设计特异引物，转化成新的SCAR标记。获得的SCAR标记S6B3，具有很高的检测稳定性，在回交群体Popu2上分析验证，结果与AFLP标记完全一致。该标记与不育基因相距0.3 cM，将其用于临保系同源的纯合型不育系选育，可有效提高育种工作效率。

关键词: 甘蓝型油菜, 显性核不育, EST, AFLP, SCAR

Abstract:

Dominant genic male sterility (Ms) in Brassica napus has been widely utilized in recurrent selection and in heterosis application. Recent genetical studies verified that its restorer gene is an allele locating at the Ms gene locus. According to this genetic pattern, a whole sterile population (Msms) can be acquired by crossing a homozygous male sterile line (MsMs) with a temporary maintainer (msms) and further used as a female parent in hybrid production, but trans-breeding of the sterile line or the temporary maintainer line that has the same nuclear background with the temporary maintainer line or the sterile line is critical to obtain uniform hybrid population and to maintain heterosis. Because of being laborious and time-consuming, an AFLP marker is usually converted to a PCR marker which is more efficient in molecular marker-assisted selection. In present study, we developed a SCAR marker with bioinformatics method from an AFLP marker SA12MG14 tightly linked to the Ms. Homologous sequences for this marker were obtained through Blast search (http://www.ncbi.nlm.nih.gov), and a corresponding accession of EST from Brassica napus was found from the Arabidopsis thaliana Integrated Database (http://atidb.org/cgi-perl/gbrowse/atibrowse). According to the combined sequence information of the AFLP fragment and the EST, a pair of primers was designed and analyzed on a backcross population Popu2. A dominant SCAR marker S6B3 was successfully identified and further detected consistently on the population with the original AFLP marker. The detected band was clear and steady. This marker is 0.3 cM away from the Ms, and its practical application will enhance work efficiency of breeding for homozygous sterile lines homologous to corresponding temporary maintainers.

Key words: Brassica napus, Dominant genic male sterility, EST, AFLP, SCAR

[1]Song L-Q(宋来强), Fu T-D(傅廷栋), Yang G-S(杨光圣), Tu J-X(涂金星), Ma C-Z(马朝芝). Genetic verification of multiple allelic gene for dominant genic male sterility in 609AB (Brassica napus L.). Acta Agron Sin (作物学报), 2005, 31(7): 869-875(in Chinese with English abstract)
[2] Song L-Q(宋来强), Fu T-D(傅廷栋), Yang G-S(杨光圣), Tu J-X(涂金星), Ma C-Z(马朝芝). Allelism analysis of dominant genic male sterility gene and its restorer gene in Brassica napus. Sci Agric Sin (中国农业科学), 2006, 39(3): 456-462(in Chinese with English abstract)
[3] Lu G-Y(陆光远),Yang G-S(杨光圣),Fu T-D(傅廷栋). Identification of AFLP markers linked to the dominant genic male sterility gene in Brassica napus L. Acta Agron Sin (作物学报), 2004, 30(2): 104-107(in Chinese with English abstract)
[4] Lu G-Y(陆光远),Yang G-S(杨光圣),Fu T-D(傅廷栋). Linkage map construction and mapping of a dominant genic male sterility gene Ms in Brassica napus. Acta Genet Sin (遗传学报), 2004, 31(11): 1309-1315(in Chinese with English abstract)
[5] Lu GY, Yang GS, Fu TD. Molecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassica napus L.). Plant Breed, 2004, 123: 262-265
[6] Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLP: A new technique for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407-4414
[7] Song L Q, Fu T D, Tu J X, Ma C Z, Yang G S. Molecular validation of multiple allele inheritance for dominant genic male sterility gene in Brassica napus L. Theor Appl Genet, 2006, 113: 55-62
[8] Shirasawa K, Kishitani S, Nishio T. Conversion of AFLP markers to sequence-specific markers for closely related lines in rice by use of the rice genome sequence. Mol Breed, 2004, 14: 283-292
[9] Inoue H, Nishio T. Efficiency of PCR-RF-SSCP marker production in Brassica oleracea using Brassica EST sequences. Euphytica, 2004, 137: 233-242
[10] Yu J K, Rota M L, Kantety R V, Sorrells M E. EST derived SSR markers for comparative mapping in wheat and rice. Mol Gen Genomics, 2004, 271: 742-751
[11] Zhang L Y, Bernard M, Leroy P, Feuillet C, Sourdille P. High transferability of bread wheat EST-derived SSRs to other cereals. Theor Appl Genet, 2005, 111: 677-687
[12] Nicot N, Chiquet V, Gandon B, Amilhat L, Legeai F, Leroy P, Bernard M, Sourdille P. Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs). Theor Appl Genet, 2004, 109: 800-805
[13] Peng J H, Lapitan N L V. Characterization of EST-derived microsatellites in the wheat genome and development of eSSR markers. Funct Integr Genomics, 2005, 5: 80-96 Kim J S, Chung T Y, King G J, Jin M, Yang T J, Jin Y M, Kim H I, Park B S. A sequence-tagged linkage map of Brassica rapa. Genetics, 2006, 174: 29-39
[1] 马毅娜, 吴晓明玉, 李藕琪, 王圆, 陈丽, 张盈川, 赵伦, 文静, 傅廷栋, 沈金雄. Bna-miR1040-EIF3A模块调控油菜开花时间的功能研究[J]. 作物学报, 2026, 52(2): 349-362.
[2] 王彬, 蒙姜宇, 邱浩良, 贺亚军, 钱伟. 甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析[J]. 作物学报, 2025, 51(8): 2100-2110.
[3] 夏琦, 郭滢, 王坤美, 王思忆, 巨建业, 彭雅雯, 刘忠松, 夏石头. 甘蓝型油菜种子和种皮中水杨酸含量与原花色素积累的关系研究[J]. 作物学报, 2025, 51(5): 1189-1197.
[4] 王晓琳, 刘忠松, 康雷, 杨柳. 甘蓝型油菜角果长度和每角粒数基因定位以及角果皮转录组动态分析[J]. 作物学报, 2025, 51(4): 888-899.
[5] 张琴, 戴成, 马朝芝. 生长素响应报告基因转化甘蓝型油菜及各组织GUS动态信号分析[J]. 作物学报, 2025, 51(3): 667-675.
[6] 展宗冰, 靳奇峰, 刘迪, 吕迎春, 郭莹, 张雪婷, 虎梦霞, 王尚, 杨芳萍. 甘肃省小麦农家种老芒麦分子鉴定及其重要性状评价[J]. 作物学报, 2025, 51(3): 609-620.
[7] 孙程明, 周晓婴, 陈锋, 张维, 王晓东, 彭琦, 郭月, 高建芹, 胡茂龙, 付三雄, 张洁夫. 长链非编码RNA (lncRNA)在甘蓝型油菜分枝角度调控中的功能分析与预测[J]. 作物学报, 2025, 51(3): 559-567.
[8] 黄绒, 周渠晨, 陈楚铭, 罗倩, 易东, 杜常欢, 黄祥宇, 盛锋, 杜雪竹. 过表达BnNRT2.3-like对油菜氮素吸收利用及产量的影响[J]. 作物学报, 2025, 51(12): 3184-3197.
[9] 巨建业, 杨柳, 陈浩, 康雷, 夏石头, 刘忠松. 单细胞核转录组分析揭示油菜种皮分化过程和种子颜色差异原因[J]. 作物学报, 2025, 51(11): 2860-2874.
[10] 张雯, 李玉, 王创, 石磊, 丁广大. 甘蓝型油菜磷转运蛋白BnaPT48的功能研究[J]. 作物学报, 2025, 51(11): 2983-2995.
[11] 胡志康, 舒雨, 王会, 杨莹莹, 廖俊宇, 刘佳, 成洪涛, 郭晨, 张园园, 刘胜毅, 胡琼, 梅德圣, 李超. 甘蓝型油菜苗期耐碱性种质综合鉴定与评价[J]. 作物学报, 2025, 51(10): 2681-2692.
[12] 王晨, 贺丹, 姚敏, 邱萍, 何昕, 熊兴华, 康雷, 刘忠松, 钱论文. 基于转录组分析鉴定甘蓝型油菜开花候选基因以及BnaCOR27功能验证[J]. 作物学报, 2025, 51(10): 2693-2704.
[13] 徐林珊, 郜耿东, 王宇, 王家星, 杨吉招, 武亚瑞, 张宵寒, 常影, 李真, 谢雄泽, 龚德平, 王晶, 葛贤宏. 甘蓝型油菜漆酶基因家族成员表达模式及与茎秆抗折力的关联分析[J]. 作物学报, 2025, 51(1): 134-148.
[14] 李嘉欣, 黄莹, 吴潞梅, 赵伦, 易斌, 马朝芝, 涂金星, 沈金雄, 傅廷栋, 文静. 甘蓝型油菜BnaSLY1基因进化分析及功能研究[J]. 作物学报, 2025, 51(1): 44-57.
[15] 钟元, 朱天宇, 戴成, 马朝芝. 耐亚磷酸盐除草剂转基因油菜的创建和抗性评价[J]. 作物学报, 2024, 50(5): 1158-1171.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2