欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (8): 1458-1461.doi: 10.3724/SP.J.1006.2009.01458

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

EST辅助的甘蓝型油菜显性核不育AFLP标记转化

宋来强1,2,易斌2,杨明贵2,陈伦林1,傅廷栋2   

  1. 1江西省农业科学院油料作物重点实验室，江西南昌330200；2华中农业大学植物遗传改良国家重点实验室，高家油菜品种改良武汉分中心，湖北武汉430070
  • 收稿日期:2008-12-17 修回日期:2009-03-08 出版日期:2009-08-12 网络出版日期:2009-05-19
  • 作者简介:songlaiqiang@yahoo.com.cn
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2001CB10807），引进国际先进农业科学技术计划（948计划）项目（2006-G04），高家油菜现代产业技术体系（nycytx-00512）项目资助。

EST-Aided Conversion of AFLP Marker Linked to Dominant Male Sterility Gene in Brassica napus into SCAR

SONG Lai-Qiang1,2,YI Bin2,YANG Ming-Gui2,CHEN Lun-Lin1,FU Ting-Dong2   

  1. 1Key Laboratory of Oil Crops，jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;2National Key Laboratory of Crop Genetic Improvement,National Subcenter of Rapeseed Improvement in Wuhan,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2008-12-17 Revised:2009-03-08 Published:2009-08-12 Published online:2009-05-19
  • About author:songlaiqiang@yahoo.com.cn

PDF

1402

被引次数

13

摘要：

甘蓝型油菜显性核不育广泛应用于轮回选择和杂种优势利用，不育基因标记的开发与应用对于基因克隆和育种实践具有重要意义。基于AFLP标记SA12MG14的序列信息，从拟南芥整合数据库中，检索与标记序列同源的甘蓝型油菜EST，结合标记和EST序列设计特异引物，转化成新的SCAR标记。获得的SCAR标记S6B3，具有很高的检测稳定性，在回交群体Popu2上分析验证，结果与AFLP标记完全一致。该标记与不育基因相距0.3 cM，将其用于临保系同源的纯合型不育系选育，可有效提高育种工作效率。

关键词: 甘蓝型油菜, 显性核不育, EST, AFLP, SCAR

Abstract:

Dominant genic male sterility (Ms) in Brassica napus has been widely utilized in recurrent selection and in heterosis application. Recent genetical studies verified that its restorer gene is an allele locating at the Ms gene locus. According to this genetic pattern, a whole sterile population (Msms) can be acquired by crossing a homozygous male sterile line (MsMs) with a temporary maintainer (msms) and further used as a female parent in hybrid production, but trans-breeding of the sterile line or the temporary maintainer line that has the same nuclear background with the temporary maintainer line or the sterile line is critical to obtain uniform hybrid population and to maintain heterosis. Because of being laborious and time-consuming, an AFLP marker is usually converted to a PCR marker which is more efficient in molecular marker-assisted selection. In present study, we developed a SCAR marker with bioinformatics method from an AFLP marker SA12MG14 tightly linked to the Ms. Homologous sequences for this marker were obtained through Blast search (http://www.ncbi.nlm.nih.gov), and a corresponding accession of EST from Brassica napus was found from the Arabidopsis thaliana Integrated Database (http://atidb.org/cgi-perl/gbrowse/atibrowse). According to the combined sequence information of the AFLP fragment and the EST, a pair of primers was designed and analyzed on a backcross population Popu2. A dominant SCAR marker S6B3 was successfully identified and further detected consistently on the population with the original AFLP marker. The detected band was clear and steady. This marker is 0.3 cM away from the Ms, and its practical application will enhance work efficiency of breeding for homozygous sterile lines homologous to corresponding temporary maintainers.

Key words: Brassica napus, Dominant genic male sterility, EST, AFLP, SCAR

[1]Song L-Q(宋来强), Fu T-D(傅廷栋), Yang G-S(杨光圣), Tu J-X(涂金星), Ma C-Z(马朝芝). Genetic verification of multiple allelic gene for dominant genic male sterility in 609AB (Brassica napus L.). Acta Agron Sin (作物学报), 2005, 31(7): 869-875(in Chinese with English abstract)
[2] Song L-Q(宋来强), Fu T-D(傅廷栋), Yang G-S(杨光圣), Tu J-X(涂金星), Ma C-Z(马朝芝). Allelism analysis of dominant genic male sterility gene and its restorer gene in Brassica napus. Sci Agric Sin (中国农业科学), 2006, 39(3): 456-462(in Chinese with English abstract)
[3] Lu G-Y(陆光远),Yang G-S(杨光圣),Fu T-D(傅廷栋). Identification of AFLP markers linked to the dominant genic male sterility gene in Brassica napus L. Acta Agron Sin (作物学报), 2004, 30(2): 104-107(in Chinese with English abstract)
[4] Lu G-Y(陆光远),Yang G-S(杨光圣),Fu T-D(傅廷栋). Linkage map construction and mapping of a dominant genic male sterility gene Ms in Brassica napus. Acta Genet Sin (遗传学报), 2004, 31(11): 1309-1315(in Chinese with English abstract)
[5] Lu GY, Yang GS, Fu TD. Molecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassica napus L.). Plant Breed, 2004, 123: 262-265
[6] Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLP: A new technique for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407-4414
[7] Song L Q, Fu T D, Tu J X, Ma C Z, Yang G S. Molecular validation of multiple allele inheritance for dominant genic male sterility gene in Brassica napus L. Theor Appl Genet, 2006, 113: 55-62
[8] Shirasawa K, Kishitani S, Nishio T. Conversion of AFLP markers to sequence-specific markers for closely related lines in rice by use of the rice genome sequence. Mol Breed, 2004, 14: 283-292
[9] Inoue H, Nishio T. Efficiency of PCR-RF-SSCP marker production in Brassica oleracea using Brassica EST sequences. Euphytica, 2004, 137: 233-242
[10] Yu J K, Rota M L, Kantety R V, Sorrells M E. EST derived SSR markers for comparative mapping in wheat and rice. Mol Gen Genomics, 2004, 271: 742-751
[11] Zhang L Y, Bernard M, Leroy P, Feuillet C, Sourdille P. High transferability of bread wheat EST-derived SSRs to other cereals. Theor Appl Genet, 2005, 111: 677-687
[12] Nicot N, Chiquet V, Gandon B, Amilhat L, Legeai F, Leroy P, Bernard M, Sourdille P. Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs). Theor Appl Genet, 2004, 109: 800-805
[13] Peng J H, Lapitan N L V. Characterization of EST-derived microsatellites in the wheat genome and development of eSSR markers. Funct Integr Genomics, 2005, 5: 80-96 Kim J S, Chung T Y, King G J, Jin M, Yang T J, Jin Y M, Kim H I, Park B S. A sequence-tagged linkage map of Brassica rapa. Genetics, 2006, 174: 29-39
[1] 陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371.
[2] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[3] 秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[4] 袁大双, 邓琬玉, 王珍, 彭茜, 张晓莉, 姚梦楠, 缪文杰, 朱冬鸣, 李加纳, 梁颖. 甘蓝型油菜BnMAPK2基因的克隆及功能分析[J]. 作物学报, 2022, 48(4): 840-850.
[5] 黄成, 梁晓梅, 戴成, 文静, 易斌, 涂金星, 沈金雄, 傅廷栋, 马朝芝. 甘蓝型油菜BnAPs基因家族成员全基因组鉴定及分析[J]. 作物学报, 2022, 48(3): 597-607.
[6] 王瑞, 陈雪, 郭青青, 周蓉, 陈蕾, 李加纳. 甘蓝型油菜白花基因InDel连锁标记开发[J]. 作物学报, 2022, 48(3): 759-769.
[7] 余国武, 青芸, 何珊, 黄玉碧. 玉米SSIIb蛋白多克隆抗体的制备及其应用[J]. 作物学报, 2022, 48(1): 259-264.
[8] 王艳花, 刘景森, 李加纳. 整合GWAS和WGCNA筛选鉴定甘蓝型油菜生物产量候选基因[J]. 作物学报, 2021, 47(8): 1491-1510.
[9] 李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明. 新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定[J]. 作物学报, 2021, 47(5): 789-798.
[10] 唐鑫, 李圆圆, 陆俊杏, 张涛. 甘蓝型油菜温敏细胞核雄性不育系160S花药败育的形态学特征和细胞学研究[J]. 作物学报, 2021, 47(5): 983-990.
[11] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状[J]. 作物学报, 2021, 47(4): 587-598.
[12] 李书宇, 黄杨, 熊洁, 丁戈, 陈伦林, 宋来强. 甘蓝型油菜早熟性状QTL定位及候选基因筛选[J]. 作物学报, 2021, 47(4): 626-637.
[13] 张春, 赵小珍, 庞承珂, 彭门路, 王晓东, 陈锋, 张维, 陈松, 彭琦, 易斌, 孙程明, 张洁夫, 傅廷栋. 甘蓝型油菜千粒重全基因组关联分析[J]. 作物学报, 2021, 47(4): 650-659.
[14] 唐婧泉, 王南, 高界, 刘婷婷, 文静, 易斌, 涂金星, 傅廷栋, 沈金雄. 甘蓝型油菜SnRK基因家族生物信息学分析及其与种子含油量的关系[J]. 作物学报, 2021, 47(3): 416-426.
[15] 蒙姜宇, 梁光伟, 贺亚军, 钱伟. 甘蓝型油菜耐盐和耐旱相关性状的QTL分析[J]. 作物学报, 2021, 47(3): 462-471.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2