陆地棉产量相关性状的QTL定位

doi:10.3724/SP.J.1006.2009.01812

摘要/Abstract

摘要：

中棉所28和湘杂棉2号分别是以中棉所12×4133和中棉所12×8891配制而成的两个陆地棉强优势杂交种。以其F₂为作图群体，筛选6000多对SSR引物，利用两群体间27个共有多态位点，通过JoinMap 3.0软件整合了一张包含245个多态位点、全长1847.81 cM的遗传图谱。利用Win QTLCart 2.5复合区间作图法分别对两群体8个产量相关性状在F₂和F_2:3中进行QTL定位，在中棉所28群体多环境平均值的联合分析中检测到16个QTL，三环境分离分析中检测到43个QTL；在湘杂棉2号群体分别检测到20个和66个QTL。在A3、D8、D9等染色体上有QTL成簇分布现象，同时在两个群体中发现一些不受环境影响且稳定遗传的QTL。对考察的8个性状在两个群体中发现12对共有QTL，控制果枝数、衣分和籽指的QTL增效基因位点均来源于共同亲本中棉所12。综合分析推测中棉所12的育种价值主要是通过提高后代的结铃性来实现的。研究结果为棉花产量性状的分子设计育种提供了有用的信息。

关键词: 棉花, 产量性状, QTL定位

Abstract:

As a major source of fiber and the world’s second-most important oil-seed crop after soybean, cotton plays an important role in the global economy. With the development of textile technology and social demand, it is urgent to breed and plant cotton varieties with high yield and super fiber quality. If the marker tightly linked with major gene controlling desired traits was identified, the efficiency of selection for agronomic traits might improve greatly. So far, high-identity genetic linkage map derived from upland cotton cultivars was lack because of their narrow genetic basis. Increasing the map density and tagging QTLs related with agronomic traits in Upland cotton will accelerate the process of marker assisted selection (MAS) breeding. CRI28 and XZM 2 are two cotton hybrids with high heterosis, which were bred by crossing CRI12, 4133, and 8891, respectively, with CRI12 as mutual parent. In this paper, two F₂ mapping populations were respectively assembled by using the parents of CRI28 hybrid (CRI12 and 4133, their corresponding population named as Pop1) and the parents of XZM 2 hybrid (CRI12 and 8891, their corresponding population named as Pop2), further, a joinmap linkage map which contained 245 loci and covered 1 847.81 cM was integrated with 27 mutual polymorphic loci in the two mapping populations by JOINMAP 3.0 software, after screening about 6 000 SSR primers. By the composite interval mapping method (CIM), the QTLs for eight yield-related traits in F₂ and F₃ populations were mapped. Of them, 43 QTLs were identified in the three environments by separating analysis and 16 QTLs by the joint analysis in Pop1; similarly, 66 and 20 QTLs in Pop2 respectively. Some QTLs on chromosome A3, D8, and D9, and some stable QTLs not influenced by environment were also detected. Twelve QTLs for eight traits could be found simultaneously in the two populations, and additive QTLs for fruit branches per plant, lint percentage and seed index all were offered by CRI12, suggesting that the value of CRI12 in breeding is mostly contributed by increasing the offspring’s bolls. These results will provide very important information in Upland cotton breeding for yield by MAS.

Key words: Upland cotton, Yield traits, QTL mapping

秦永生,刘任重,梅鸿献,张天真,郭旺珍*. 陆地棉产量相关性状的QTL定位[J]. 作物学报, 2009, 35(10): 1812-1821.

QIN Yong-Sheng,LIU Ren-Zhong,MEI Hong-Xian,ZHANG Tian-Zhen,GUO Wang-Zhen. QTL Mapping for Yield Traits in Upland Cotton(Gossypium hirsutum L.)[J]. Acta Agron Sin, 2009, 35(10): 1812-1821.

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