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作物学报 ›› 2011, Vol. 37 ›› Issue (12): 2299-2305.doi: 10.3724/SP.J.1006.2011.02299

• 研究简报 • 上一篇    

棉花杂交种纯度的SSR标记检测及其与田间表型鉴定的相关性

匡猛,杨伟华*,张玉翠,许红霞,王延琴,周大云,冯新爱,苏畅,周红   

  1. 中国农业科学院棉花研究所 / 棉花生物学国家重点实验室，河南安阳 455000
  • 收稿日期:2011-06-13 修回日期:2011-09-18 出版日期:2011-12-12 网络出版日期:2011-09-29
  • 通讯作者: 杨伟华, E-mail: yangwh @cricaas.com.cn, Tel: 0372-2525389
  • 基金资助:

    本研究由中央级公益性科研院所基本科研业务费专项重点项目(SJA0904)和农业部种子农药质量安全监管专项(农财发[2011]50号)资助。

Cotton Hybrid Purity Tested by SSR Markers and Its Correlation with Phenotype Identification in Field

KUANG Meng,YANG Wei-Hua*,ZHANG Yu-Cui,XU Hong-Xia,WANG Yan-Qin,ZHOU Da-Yun,FENG Xin-Ai,SU Chang,ZHOU Hong   

  1. Cotton Research Institute, Chinese Academy of Agriculture Sciences / State Key Laboratory of Cotton Biology, Anyang, 455000, China
  • Received:2011-06-13 Revised:2011-09-18 Published:2011-12-12 Published online:2011-09-29
  • Contact: 杨伟华, E-mail: yangwh @cricaas.com.cn, Tel: 0372-2525389

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被引次数

27

摘要： 以分布于棉花26条染色体的36对SSR核心引物，在6份成套中棉所系列杂交种间筛选双亲互补型杂合位点作为纯度检测标记, 采用单位点平均法与双位点差异法统计SSR标记检测结果; 在棉铃期考察棉花株型、铃形、叶形、花及茎等性状, 比对田间表型与分子检测结果, 分析相关性。结果表明, 34对核心引物在6个杂交种上获得101个杂合位点，平均每个杂交种具有16.8个。田间表型鉴定结果高于分子标记检测结果，单位点平均法的相关性高于双位点差异法，校正后的相关性进一步提高，相关系数r=0.7841，呈高度相关。EST-SSR标记的检测结果与田间表型鉴定结果的相关性显著高于Genomic-SSR标记。相同染色体上的不同引物检测结果差异较小。不同的统计方法与标记类型获得的分子标记检测结果差异较大。高度纯合的亲本将会有效提高分子标记鉴定结果与表型鉴定结果的相关性。

关键词: 棉花, 杂交种, SSR, 形态学, 纯度

Abstract: Cotton is one of the most important cash corps. Hybrid cotton is extensively grown in China. Hybrid seeds are generally planted and used for purity identification. The processtakes long time and much money. Therefore, it is important to explore a new method to inspect the purity of hybrid cotton. In order to explore the validity of hybrid cotton purity identification using SSR marker technique, the purity for six hybrids was tested by SSR approach and morphological assessment, and the correlation between the two methods was researched. Primers with complementary band type screened out from 36 evenly distributed SSR pairs were applied in purity identification. Morphological traits including plant type, boll, leaf shape, flower and stem were observed. Two statistics methods were used for testingpurity based on SSR marker, and the results were compared to that of morphological identification. Among the six hybrid cottons, 34 primer pairs had 101 heterozygous loci, with an average of 16.8 heterozygous loci for on each hybrid. The purity based on morphology was higher than that on SSR markers, the results of “Single locus on average” (the average value of purity test by each SSR locus) had a higher correlation with morphology compared to “Double locus difference” (if more than two SSR loci of an individual are different from the tested cultivar’s, the individual is considered as farraginous plant), and the correlation would be better after calibration (correlation coefficient reached to 0.7841). Correlation based on EST-SSR was much better than that on Genomic-SSR. There was certain relativity at different loci of the same chromosome. The purity was relevant to different statistics method and marker type. There will be a good correlation for purity identification between methods of SSR marker and morphology if the hybrid parents are highly pure.

Key words: Cotton, Hybrid, SSR, Morphology, Purity

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