小麦/玉米/大豆和小麦/玉米/甘薯套作对根际土壤细菌群落多样性及植株氮素吸收的影响

doi:10.3724/SP.J.1006.2012.00333

摘要/Abstract

摘要： 为探讨小麦/玉米/大豆和小麦/玉米/甘薯套作体系中根际细菌群落多样性与作物氮素高效吸收的差异特性及二者间的关系，应用变性梯度凝胶电泳(DGGE)技术研究了小麦–大豆(A1)、小麦–甘薯(A2)、玉米(A3)、小麦/玉米/大豆(A4)和小麦/玉米/甘薯(A5)5种种植模式的根际细菌群落多样性。结果表明，与A1、A2、A3及A5相比，A4套作提高了各作物在开花期(或吐丝期)与成熟期的籽粒吸氮量、地上部总吸氮量和Shannon-Weiner index多样性指数(H′)。处理间的吸氮量与H′的变化规律为套作>单作、大豆茬口>甘薯茬口，以A4处理最高。不同种植模式下DGGE图谱条带的数量及亮度有较大区别，且有几条特征性条带发生了明显变化。不同种植模式间的细菌群落结构相似性较低，群落相似度系数(Cs)表现为套作与套作间>套作与单作间；A4与A5间的Cs相对较小，二者间的细菌群落结构差异较大。A4模式有利于提高根际细菌群落多样性，增强植株对氮素的吸收能力。

关键词: 小麦/玉米/大豆套作, 氮素吸收, 细菌群落多样性, PCR-DGGE

Abstract: The aim of this study was to understand the relationship between diversity of rhizosphere bacterial community and nitrogen uptake of crops in two relay strip intercropping systems: wheat/maize/soybean and wheat/maize/sweet potato. We analyzed the diversities of rhizosphere bacterial community in five cropping systems using denaturing gradient gel electrophoresis (DGGE) based on 16S rDNA. The cropping systems included wheat-soybean (A1), wheat-sweet potato (A2), maize single cropping (A3), wheat/maize/soybean (A4), and wheat/maize/sweet potato (A5). Compared to the sole cropping systems (A1, A2, and A3 treatments), the A4 treatment showed increases in grain nitrogen uptake and total nitrogen uptake amounts of aboveground of crops at both flowering (or silking) and maturity stages, and the Shannon-Weiner indices for rhizosphere bacterial community diversity was also increased significantly. The values of nitrogen uptake and the Shannon-weiner index in the relay strip intercropping systems were greater than those in sole cropping systems, and the values in the treatment with soybean stubble were greater than those in the treatment with sweet potato stubble. The number and strength of the DNA bands from DGGE profiles varied with the cropping systems, especially for the distinguished bands. The comparability of rhizosphere bacterial community composition was low among different cropping systems. The similarity coefficient (Cs) was higher between two relay strip intercropping systems than between relay strip intercropping system and sole cropping system, but the Cs value was low between A4 and A5 treatments. However, there was obvious difference of rhizosphere bacterial community composition between A4 and A5 treatments, and the A4 treatment was propitious to enhancing rhizosphere bacterial community diversity and increasing nitrogen uptakes of crops.

Key words: Wheat/maize/soybean relay strip intercropping, Nitrogen uptake, Bacterial community diversity, PCR-DGGE

雍太文, 杨文钰, 向达兵, 朱贞颖. 小麦/玉米/大豆和小麦/玉米/甘薯套作对根际土壤细菌群落多样性及植株氮素吸收的影响[J]. 作物学报, 2012, 38(02): 333-343.

YONG Ta-Wen, YANG Wen-Yu, XIANG Da-Bing, ZHU Zhen-Ying. Effect of Wheat/Maize/Soybean and Wheat/Maize/Sweet Potato Relay Strip Intercropping on Bacterial Community Diversity of Rhizosphere Soil and Nitrogen Uptake of Crops[J]. Acta Agron Sin, 2012, 38(02): 333-343.

参考文献

[1]Li L, Sun J H, Zhang F S, Li X L, Rengel Z, Yang S C. wheat/maize or wheat/soybean strip intercropping: I. Yield advantage and interspecific interactions on nutrients. Field Crops Res, 2001, 71: 123–137
[2]Eaglesham A R J, Ayanaba A, Rao V R. Improving the nitrogen nutrition of maize by intercropping with cowpea. Soil Biol Biochem, 1981, 13: 169–171
[3]Gianfrda L, Sannino F, Violante A. Pesticide effects on the activity of free immobilized and soil invertase. Soil Biol Biochem, 1995, 27: 1201–1208
[4]Li X-Y(李秀英), Zhao B-Q(赵秉强), Li X-H(李絮花), Li Y-T(李燕婷), Sun R-L(孙瑞莲), Zhu L-S(朱鲁生), Xu J(徐晶), Wang L-X(王丽霞), Li X-P(李小平), Zhang F-D(张夫道). Effects of different fertilization systems on soil microbe and its relation to soil fertility. Sci Agric Sin (中国农业科学), 2005, 38(8): 1591–1599 (in Chinese with English abstract)
[5]Yong T-W(雍太文), Yang W-Y(杨文钰), Ren W-J(任万军), Fan G-Q(樊高琼), Xiang D-B(向达兵). Analysis of the nitrogen transfer, nitrogen uptake and utilization in the two relay-planting systems. Sci Agric Sin (中国农业科学), 2009, 42(9): 3170–3178 (in Chinese with English abstract)
[6]Yong T-W(雍太文), Yang W-Y(杨文钰), Fan G-Q(樊高琼), Wang X-C(王小春), Zhang Y-F(张亚飞). Study on the annual balance application of nitrogen fertilizer in the relay-cropping system of “wheat/maize/soybean”. Soil & Fert Sci China (中国土壤与肥料), 2009, (3): 31–35 (in Chinese with English abstract)
[7]Li L(李隆), Yang S-C(杨思存), Sun J-H(孙建好), Li X-L(李晓林), Zhang F-S(张福锁). Interspecific competition and facilitation in wheat/soybean intercropping system. Chin J Appl Ecol (应用生态学报), 1999, 10(2): 197–200 (in Chinese with English abstract)
[8]Li W X, Li L, Sun J H, Guo T W, Zhang F S, Bao X G, Peng A, Tang C. Effects of intercropping and nitrogen application on nitrate present in the profile of an Orthic Anthrosol in Northwest China. Agric Ecosyst & Environ, 2005, 105: 483–49
[9]Ye Y-L(叶优良),Li L (李隆), Suo D-R(索东让). Effect of wheat/maize and faba bean/maize intercropping on soil nitrate nitrogen concentration and accumulation. Ecol & Environ (生态环境), 2008, 17(1): 377–383 (in Chinese with English abstract)
[10]Wang Y-F(王艳芳). Progress of research on soil nitrogen transfer and movement. J Ningxia Agric Coll (宁夏农学院学报), 2004, 25(1): 53-56 (in Chinese with English abstract)
[11]Hatfield J L, Stewart B A. Soil Biology: Effects on Soil Quality. Boca Raton, USA: Lewis Publishers, 1994
[12]Sugden A M. Ecology: diversity and ecosystem resilience. Science, 2000, 290: 233–235
[13]Walter K D, Margaret K B, Courtney S C. Biological properties of soil and subsurface sediments under abandoned pasture and cropland. Soil Biol Biochem, 1997, 28: 837–846
[14]Song Y-N(宋亚娜), Marschner P, Zhang F-S(张福锁), Bao X-G(包兴国), Li L(李隆). Effect of intercropping on bacterial community composition in rhizoshpere of wheat (Triticum aestivum L.), maize (Zea mays L.) and faba bean (Vicia faba L.). Acta Ecol Sin (生态学报), 2006, 26(7): 2268–2274 (in Chinese with English abstract)
[15]Zhang J-E(章家恩), Gao A-X(高爱霞), Xu H-Q(徐华勤), Luo M-Z(罗明珠). Effects of maize/peanut intercropping on rhizosphere soil microbes and nutrient contents. Chin J Appl Ecol (应用生态学报), 2009, 20(7): 1597–1602 (in Chinese with English abstract)
[16]Xie K-Z(解开治), Xu P-Z(徐培智), Li K-H(李康活), Zhou S-C(周少川), Chen J-S(陈建生), Tang S-H(唐拴虎), Zhang F-B(张发宝), Huang X(黄旭). Effects of three different cropping system on diversity of soil bacterial community. Plant Nutr Fert Sci (植物营养与肥料学报), 2009, 15(5): 1107–1113 (in Chinese with English abstract)
[17]Gu Y-F(辜运富), Zhang X-P(张小平), Tu S-H(涂仕华), Sun X-F(孙锡发), Kristina L. Effect of long-term fertilization on nitrification and nitrobacteria community in a purple paddy soil under rice-wheat rotations. Acta Ecol Sin (生态学报), 2008, 28(5): 2123–2130 (in Chinese with English abstract)
[18]Chen Z(陈哲), Yuan H-Z(袁红朝), Wu J-S(吴金水), Wei W-X(魏文学). Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization. Acta Ecol Sin (生态学报), 2009, 29(11): 5923–5929 (in Chinese with English abstract)
[19]Li Y(李杨), Cen J(岑剑), Wang B-L(王保莉), Qu D(曲东). RFLP analysis on the impact of microbial diversity of dry land soils without nitrogenous fertilizer. Agric Res Arid Areas (干旱地区农业研究), 2009, 27(2): 199–203 (in Chinese with English abstract)
[20]Luo H F, Qi H Y, Zhang H X. Assessment of the bacterial diversity in fenvalerate-treated soil. World J Microbiol Biotechnol, 2004, 20: 509–515
[21]Xiao Y B, Li L, Zhang F S. Effect of root contact on interspecific competition and N transfer between wheat and faba bean using direct and indirect 15N techniques. Plant & Soil, 2004, 262: 45–54
[22]Chu G-X(褚贵新), Shen Q-R(沈其荣), Li Y-L(李奕林), Zhang J(张娟), Wang S-Q(王树起). Researches on Bi-directional N transfer between the intercropping system of groundnut with rice cultivated in aerobic soil using 15N foliar labelling method. Acta Ecol Sin (生态学报), 2004, 24(2): 278–284 (in Chinese with English abstract)
[23]Li S-M(李少明), Zhao P(赵平), Fan M-P(范茂攀), Gao S-C(高世昌), Zheng Y(郑毅). Nitrogen uptake and utilization in intercropping system of maize and soybean. J Yunnan Agric Univ (云南农业大学学报), 2004, 19(5): 527–574 (in Chinese with English abstract)
[24]Xiao Y-B(肖焱波), Li L(李隆), Zhang F-S(张福锁). An outlook of the complementary nitrogen nutrition in the legume// graminaceae System. Rev China Agric Sci & Technol (中国农业科技导报), 2003, 5(6): 44–49 (in Chinese with English abstract)
[25]Abbott L K, Murphy D V. Soil Biological Fertility. Netherlands: Kluwer Academic Publishers, 2003
[26]Wang Y(王瑛), Meng Y-L(孟亚利), Chen B-L(陈兵林), Zhou Z-G(周治国), Shu H-M(束红梅), Bian H-Y(卞海云). Studies on the soil microorganism quality and soil nutrient content at the rhizosphere and non-rhizosphere region of cotton in wheat-cotton intercropping system. Acta Ecol Sin (生态学报), 2006, 26(10): 3485–3490 (in Chinese with English abstract)
[27]Dong Y(董艳), Tang L(汤利), Zheng Y(郑毅), Zhu Y-Y(朱有勇), Zhang F-S(张福锁). Effects of nitrogen application rate oil rhizosphere microbial community in wheat-faba bean intercropping system. Chin J Appl Ecol (应用生态学报), 2008, 19(7): 1559–1566 (in Chinese with English abstract)
[28]Yong T-W(雍太文), Chen X-R(陈小容), Yang W-Y(杨文钰), Xiang D-B(向达兵), Fan G-Q(樊高琼). Effect of different cropping systems on root exudates and above ground nitrogen uptake and soil nutrition of wheat. Acta Agron Sin (作物学报), 2010, 36(3): 477–485 (in Chinese with English abstract)

相关文章 15

[1]	丁红, 徐扬, 张冠初, 秦斐斐, 戴良香, 张智猛. 不同生育期干旱与氮肥施用对花生氮素吸收利用的影响[J]. 作物学报, 2022, 48(3): 695-703.
[2]	张军, 周冬冬, 许轲, 李必忠, 刘忠红, 周年兵, 方书亮, 张永进, 汤洁, 安礼政. 淮北地区麦茬机插优质食味粳稻氮肥减量的精确运筹[J]. 作物学报, 2022, 48(2): 410-422.
[3]	阮俊梅, 张俊, 刘猷红, 董文军, 孟英, 邓艾兴, 杨万深, 宋振伟, 张卫建. 田间开放式增温对东北水稻氮素利用的影响[J]. 作物学报, 2022, 48(1): 193-202.
[4]	柯健, 陈婷婷, 徐浩聪, 朱铁忠, 吴汉, 何海兵, 尤翠翠, 朱德泉, 武立权. 控释氮肥运筹对钵苗摆栽籼粳杂交稻甬优1540产量及氮肥利用的影响[J]. 作物学报, 2021, 47(7): 1372-1382.
[5]	吴冰卉, 王桂萍, 王玉斌, 李召虎, 张明才. ACC处理对不同基因型玉米幼苗响应氮素供给的调控效应[J]. 作物学报, 2021, 47(5): 799-806.
[6]	陈晓影,刘鹏,程乙,董树亭,张吉旺,赵斌,任佰朝,韩坤. 基于磷肥施用深度的夏玉米根层调控提高土壤氮素吸收利用[J]. 作物学报, 2020, 46(02): 238-248.
[7]	廖萍,刘磊,何宇轩,唐刚,张俊,曾勇军,吴自明,黄山. 施石灰和秸秆还田对双季稻产量和氮素吸收的互作效应[J]. 作物学报, 2020, 46(01): 84-92.
[8]	何丹丹,贾立国,秦永林,樊明寿. 不同马铃薯品种的氮利用效率及其分类研究[J]. 作物学报, 2019, 45(1): 153-159.
[9]	李晓峰,程金秋,梁健,陈梦云,任红茹,张洪程,霍中洋*,戴其根,许轲,魏海燕,郭保卫. 秸秆全量还田与氮肥运筹对机插粳稻产量及氮素吸收利用的影响[J]. 作物学报, 2017, 43(06): 912-924.
[10]	郭保卫,张洪程*,朱大伟,许轲霍,中洋,魏海燕,戴其根,高辉,胡雅杰,崔培媛. 有序摆抛栽水稻的氮素吸收、利用与分配特征[J]. 作物学报, 2017, 43(01): 97-111.
[11]	胡群,夏敏,张洪程,曹利强,郭保卫,魏海燕,陈厚存,戴其根,霍中洋,许轲,林昌明,韩宝富. 氮肥运筹对钵苗机插优质食味水稻产量及氮素吸收利用的影响[J]. 作物学报, 2016, 42(11): 1666-1676.
[12]	武文明，陈洪俭，王世济，魏凤珍，李金才. 氮肥运筹对苗期受渍夏玉米干物质和氮素积累与转运的影响[J]. 作物学报, 2015, 41(08): 1246-1256.
[13]	彭玉,孙永健,蒋明金,徐徽,秦俭,杨志远,马均. 不同水分条件下缓/控释氮肥对水稻干物质量和氮素吸收、运转及分配的影响[J]. 作物学报, 2014, 40(05): 859-870.
[14]	许轲,张军,花劲,张洪程,周培建,程飞虎,黄大山,陈忠平,陈国梁,戴其根,霍中洋,魏海燕,高辉. 双季杂交晚粳稻超高产形成特征[J]. 作物学报, 2014, 40(04): 678-690.
[15]	霍中洋,李杰,张洪程,戴其根,许轲,魏海燕,龚金龙. 不同种植方式下水稻氮素吸收利用的特性[J]. 作物学报, 2012, 38(10): 1908-1919.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed