作物学报 ›› 2013, Vol. 39 ›› Issue (05): 862-867.doi: 10.3724/SP.J.1006.2013.00862
苗润隆,蒋钰东,廖红香,徐芳芳,何光华,杨正林,赵芳明,桑贤春*
MIAO Run-Long,JIANG Yu-Dong,LIAO Hong-Xiang,XU Fang-Fang,HE Guang-Hua,YANG Zheng-Lin,ZHAO Fang-Ming,SANG Xian-Chun*
摘要:
叶片早衰直接降低作物的光合作用、产量和品质。因此,鉴定早衰突变体和研究其基因功能对于作物的遗传改良具有重要的作用。esl3来源于水稻籼型恢复系缙恢10号的EMS诱变库,苗期叶片中上部即呈现褐化枯萎,该特征一直持续到植株成熟。与野生型相比,突变体衰老部位叶绿素和光合速率极显著下降,绿色部位光合色素和光合速率则略有升高。农艺性状分析发现,结实率无显著变化,有效穗、穗长、穗粒数、千粒重、株高和干物质重则显著或极限著下降。遗传分析表明,esl3叶片早衰枯死性状受1对隐性核基因控制。利用391株日本晴/esl3的F2突变型单株,最终把ESL3基因定位在第5染色体SSR标记RM19085和Indel标记Ind05-2之间,物理距离91 kb,包含14个注释基因,为下一步调控基因的克隆和功能研究奠定了基础。
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