欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2013, Vol. 39 ›› Issue (12): 2135-2144.doi: 10.3724/SP.J.1006.2013.02135

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

谷子PP2C基因家族的特性

闵东红1,**,薛飞洋1, 2,**,马亚男1, 2,陈明2,徐兆师2,李连城2,刁现民2,贾冠清2,马有志2   

  1. 1西北农林科技大学农学院 / 旱区作物逆境生物学国家重点实验室，陕西杨凌712100；2中国农业科学院作物科学研究所 /农作物基因资源与基因改良国家重大科学工程 / 农业部麦类生物学与作物遗传育种重点实验室，北京100081
  • 收稿日期:2013-05-13 修回日期:2013-07-26 出版日期:2013-12-12 网络出版日期:2013-09-29
  • 通讯作者: 陈明, E-mail: chenming02@caas.cn, Tel: 13683360891
  • 基金资助:

    本研究由国家自然科学基金项目（31271715）和国家转基因新品种生物培育科技重大专项（2013ZX08002-005）项目资助。

Characteristics of PP2C Gene Family in Foxtail Millet (Setaria italica)

MIN Dong-Hong1,**,XUE Fei-Yang1,2,**,MA Ya-Nan1,2,CHEN Ming2,*,XU Zhao-Shi2,LI Lian-Cheng2,DIAO Xian-Min2,JIA Guan-Qing2,MA You-Zhi2   

  1. 1 College of Agronomy, Northwest A&F University / State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081, China
  • Received:2013-05-13 Revised:2013-07-26 Published:2013-12-12 Published online:2013-09-29

RichHTML

PDF

1627

被引次数

5

摘要：

PP2Cs (2C type protein phosphatases)是一种单体丝氨酸/苏氨酸蛋白磷酸酶，在真核生物中，PP2Cs在脱落酸(ABA)、茉莉酸(JA)、水杨酸(SA)等激素信号传导途径中起着重要的调控作用。本研究通过序列比对，从谷子基因组中筛选出80个PP2C候选基因，聚类分析将其分为12个亚族(A、B、C、D、E1、E2、F1、F2、G、H、I、J)。与拟南芥PP2C基因家族比对表明，A~I为2个物种共有的亚族，J亚族只存在于谷子基因组中，L亚族只存在于拟南芥中。将谷子A亚族的10个成员命名为SiPP2CA1-10。基因表达谱分析表明，A亚族基因不同程度受ABA、干旱、高盐、低温和低氮诱导表达，其中，SiPP2CA6、SiPP2CA8在5种处理下诱导表达量都高。对10个A亚族成员的启动子分析发现，在这些基因的启动子序列中含有多种参与逆境胁迫应答的顺式作用元件，其中，SiPP2CA5、SiPP2CA6、SiPP2CA7、SiPP2CA8的启动子中含有参与低氮胁迫响应的元件。进一步研究发现，SiPP2CA8主要在根部表达，且在低氮胁迫下一直有较高的表达水平。亚细胞定位结果显示SiPP2CA8定位在细胞膜、细胞质、细胞核中；双分子荧光互补试验(BiFC)结果表明，SiPP2CA8与一个ABA受体类似蛋白SiRCAR3(基因号Si018317m.g)在细胞膜、细胞质及细胞核上互作，表明SiPP2CA8在谷子中可能参与ABA信号传导过程。

关键词: 谷子, PP2C基因家族, 非生物胁迫, 表达分析

Abstract:

PP2Cs are a group of monomeric serine/threonine protein phosphatases, which play an important role in regulation of hormone signaling pathways such as abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA) in eukaryotes. In this study, we identified 80 candidate PP2C genes from foxtail millet genome via sequence alignment, and cluster analysis showed that PP2Cs in foxtail millet were divided into 12 subfamilies (subfamily A, B, C, D, E1, E2, F1, F2, G, H, I, and J). Foxtail millet and Arabidopsis share A, B, C, D, E1, E2, F1, F2, G, H, and I, while subfamily J is only in foxtail millet and subfamily L is only in Arabidopsis. Ten members of subfamily A in foxtail millet were named as SiPP2CA1–10, and gene expression profiles showed that the expression of these genes was induced by ABA, drought, high salt, cold and low nitrogen stresses. Among them, SiPP2CA6 and SiPP2CA8 showed high expression level in all treatments. Promoter analysis identified a variety of cis-acting elements involved in stress responses in promoter region of members in subfamily A, and a specific element responding to low nitrogen stress in SiPP2CA5, SiPP2CA6, SiPP2CA7, and SiPP2CA8. Further study demonstrated that SiPP2CA8mainly expressed in root, and its expression remained at high level under low nitrogen stress. Subcellular localization showed that SiPP2CA8 waslocalized in cytomembrane, cytoplasm and nucleus. Bimolecular fluorescence complementation (BiFC) assay demonstrated that SiPP2CA8 interacted with an ABA receptor like protein, SiRCAR3 (gene locus Si018317m.g), in cytomembrane, cytoplasm and nucleus. These results suggested that SiPP2CA8 may participate in ABA signaling pathway in foxtail millet.

Key words: Foxtail millet, PP2C gene family, Abiotic stress, Expression analysis

[1]Stern A, Privman E, Rasis M, Lavi S, Pupko T. Evolution of the metazoan protein phosphatase 2C superfamily. J Mol Evol, 2007, 64: 61–70



[2]Cohen P. The structure and regijlation of protein phosphatases. Ann Rev Biochem, 1989, 58: 453–508



[3]Schweighofer A, Hirt H, Meskiene I. Plant PP2C phosphatases: emerging functions in stress signaling. Trends Plant Sci, 2004, 9: 236–243



[4]Meskiene I, Baudouin E, Schweighofer A, Liwosz A, Jonak C, Rodriguez P L, Jelinek H, Hirt H. Stress-induced protein phosphatase 2C is a negative regulator of a mitogen-activated protein kinase. J Biol Chem, 2003, 278: 18945–18952



[5]Shi Y G. Serine/threonine phosphatases: mechanism through structure. Cell, 2009, 139: 468–484



[6]Sheen J. Mutational analysis of protein phosphatase 2C involved in abscisic acid signal transduction in higher plants. Proc Natl Acad Sci USA, 1998, 95: 975–980



[7]Schweighofer A, Kazanaviciute V, Scheikl E, Teige M, Doczi R, Hirt H, Schwanninger M, Kant M, Schuurink R, Mauch F, Buchala A, Cardinale F, Meskiene I. The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis. Plant Cell, 2007, 19: 2213–2224



[8]Nishimura N, Okamoto M, Narusaka M, Yasuda M, Nakashita H, Shinozaki K, Narusaka Y, Hirayama T. ABA hypersensitive germination2-1 causes the activation of both abscisic acid and salicylic acid responses in Arabidopsis. Plant Cell Physiol, 2009, 50: 2112–2122



[9]Hirayama T, Shinozaki K. Perception and transduction of abscisic acid signals: keys to the function of the versatile plant hormone ABA. Trends Plant Sci, 2007, 12: 343–351



[10]Meyer K, Leube M P, Grill E. A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana. Science, 1994, 264: 1452–1455



[11]Jeffrey Leung S M, and Jérôme Giraudat. The Arabidopsis ABSCISIC ACID-INSENSlTIVE2 (AB12) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell, 1997, 9: 759–771



[12]Angela Saez N A, Miguel Gonzalez Guzman, Mary Paz Gonzalez-Garcia, Carlos Nicolas, Oscar Lorenzo, Pedro L Rodriguez. Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling. Plant J, 2004, 37: 354–369



[13]Yoshida T, Nishimura N, Kitahata N, Kuromori T, Ito T, Asami T, Shinozaki K, Hirayama T. ABA-hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs. Plant Physiol, 2006, 140: 115–126



[14]Nishimura N, Yoshida T, Kitahata N, Asami T, Shinozaki K, Hirayama T. ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed. Plant J, 2007, 50: 935–949



[15]Reyes D, Rodriguez D, Gonzalez-Garcia M P, Lorenzo O, Nicolas G, Garcia-Martinez J L, Nicolas C. Overexpression of a protein phosphatase 2C from beech seeds in Arabidopsis shows phenotypes related to abscisic acid responses and gibberellin biosynthesis. Plant Physiol, 2006, 141: 1414–1424



[16]Saavedra X, Modrego A, Rodriguez D, Gonzalez-Garcia M P, Sanz L, Nicolas G, Lorenzo O. The nuclear interactor PYL8/RCAR3 of Fagus sylvatica FsPP2C1 is a positive regulator of abscisic acid signaling in seeds and stress. Plant Physiol, 2010, 152: 133–150



[17]Liu L, Hu X, Song J, Zong X, Li D, Li D. Over-expression of a Zea mays L. protein phosphatase 2C gene (ZmPP2C) in Arabidopsis thaliana decreases tolerance to salt and drought. J Plant Physiol, 2009, 166: 531–542



[18]Liu X, Zhu Y, Zhai H, Cai H, Ji W, Luo X, Li J, Bai X. AtPP2CG1, a protein phosphatase 2C, positively regulates salt tolerance of Arabidopsis in abscisic acid-dependent manner. Biochem Biophys Res Commun, 2012, 422: 710–715



[19]Jia H F, Lu D, Sun J H, Li C L, Xing Y, Qin L, Shen Y Y. Type 2C protein phosphatase ABI1 is a negative regulator of strawberry fruit ripening. J Exp Bot, 2013 (DOI:10.1093/jxb/ert1028)



[20]Xue T T, Wang D, Zhang S Z, Ehlting J, Ni F, Jakab S, Zheng C C, Zhong Y. Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis. BMC Genom, 2008, 9: 550



[21]Bhaskara G B, Nguyen T T, Verslues P E. Unique drought resistance functions of the highly ABA-induced clade A protein phosphatase 2Cs. Plant Physiol, 2012, 160: 379–395



[22]Wang Z M, Devos K M, Liu C J, Wang R Q, Gale M D. Construction of RFLP-based maps of foxtail millet, Setaria italica (L.) P. Beauv. Theor Appl Genet, 1998, 96: 31–36



[23]Doust A N, Kellogg E A, Devos K M, Bennetzen J L. Foxtail millet: a sequence-driven grass model system. Plant Physiol, 2009, 149: 137–141



[24]Bennetzen J L, Schmutz J, Wang H, Percifield R, Hawkins J, Pontaroli A C, Estep M, Feng L, Vaughn J N, Grimwood J, Jenkins J, Barry K, Lindquist E, Hellsten U, Deshpande S, Wang X W, Wu X M, Mitros T, Triplett J, Yang X H, Ye C Y, Mauro-Herrera M, Wang L, Li P, Sharma M, Sharma R, Ronald P C, Panaud O, Kellogg E A, Brutnell T P, Doust A N, Tuskan G A, Rokhsar D, Devos K M. Reference genome sequence of the model plant Setaria. Nat Biotechnol, 2012, 30: 555–561



[25]Zhang G Y, Liu X, Quan Z W, Cheng S F, Xu X, Pan S K, Xie M, Zeng P, Yue Z, Wang W L, Tao Y, Bian C, Han C L, Xia Q J, Peng X H, Cao R, Yang X H, Zhan D L, Hu J C, Zhang Y X, Li H N, Li H, Li N, Wang J Y, Wang C C, Wang R Y, Guo T, Cai Y J, Liu C Z, Xiang H T, Shi Q X, Huang P, Chen Q C, Li Y R, Wang J, Zhao Z H, Wang J. Genome sequence of foxtail millet (Setaria italica) provides insights into grass evolution and biofuel potential. Nat Biotechnol, 2012, 30: 549–554



[26]Yoo S D, Cho Y H, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Prot, 2007, 2: 1565–1572



[27]Loppes R, Radoux M. Abscisic acid biosynthesis and catabolism. Ann Rev Plant Biol, 2005, 56: 165–185



[28]Lim C W, Kim J H, Baek W, Kim B S, Lee S C. Functional roles of the protein phosphatase 2C, AtAIP1, in abscisic acid signaling and sugar tolerance in Arabidopsis. Plant Sci, 2012, 187: 83–88



[29]Ma Y, Szostkiewicz I, Korte A, Moes D, Yang Y, Christmann A, Grill E. Regulators of PP2C phosphatase activity function as abscisic acid sensors. Science, 2009, 324: 1064–1068
[1] 马小倩, 秦娜, 代书桃, 秦家范, 李小艳, 王淑婷, 刘忠玲, 李君霞. 175份谷子种质在2种环境下的表型变异分析[J]. 作物学报, 2026, 52(6): 1757-1773.
[2] 左同鸿, 张贺翠, 曾静, 朱利泉. 甘蓝自交不亲和相关基因BoPUB3L的克隆与表达分析[J]. 作物学报, 2026, 52(6): 1698-1710.
[3] 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[4] 田立涛, 丁宁, 王树林, 齐恩芳, 张荣, 王蕊蕊, 马利雯, 李建武, 杨江伟. 马铃薯Argonaute基因家族鉴定及晚疫病诱导表达分析[J]. 作物学报, 2026, 52(4): 1116-1126.
[5] 杨宗桃, 杨婷, 王禹童, 艾静, 李燕烨, 刘家勇, 邓军, 赵勇, 张跃彬. 甘蔗CLC基因家族鉴定与表达分析[J]. 作物学报, 2026, 52(3): 722-734.
[6] 孟成, 王哲. 玉米ZmPFK基因家族全基因组鉴定及响应胁迫表达分析[J]. 作物学报, 2026, 52(3): 764-779.
[7] 杨飚, 杜帅康, 张继旺, 石瑛, 张丽莉. 马铃薯III类POD基因家族的全基因组鉴定及其表达谱分析[J]. 作物学报, 2026, 52(2): 405-420.
[8] 张力岚, 杨军, 王让剑. 基于WGCNA发掘茶树糖苷类香气前体含量性状相关的候选基因[J]. 作物学报, 2026, 52(2): 494-513.
[9] 景秀清, 蔡永朵, 邓宁, 赵晓东, 翟飞红, 曾群. 藜麦RopGEF家族基因的鉴定及表达模式分析[J]. 作物学报, 2026, 52(1): 28-43.
[10] 王彬, 蒙姜宇, 邱浩良, 贺亚军, 钱伟. 甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析[J]. 作物学报, 2025, 51(8): 2100-2110.
[11] 闫知兰, 赵芹, 常甜达, 王一鸣, 王碧辉, 王鹏, 黄春国, 张会, 王利祥, 郝晓鹏, 赵波. 豆科作物AOX基因鉴定及其在普通菜豆响应非生物胁迫中的表达模式研究[J]. 作物学报, 2025, 51(7): 1769-1783.
[12] 沈傲, 刘敏, 倪迪安, 刘炜. 谷子m6A甲基转移酶基因SiMTA1的启动子序列特征和基因表达模式分析[J]. 作物学报, 2025, 51(7): 1969-1978.
[13] 王若楠, 张颖星, 于筱菡, 刘少雄, 王跃, 薛亚鹏, 辛旭霞, 张莉, 刘敏轩. 基于近红外快速检测技术的谷子淀粉多样性分析及模型构建[J]. 作物学报, 2025, 51(7): 1757-1768.
[14] 郭腾达, 崔梦杰, 陈琳杰, 韩锁义, 郭敬坤, 吴晨迪, 付留洋, 黄冰艳, 董文召, 张新友. 花生磷脂酰肌醇转运蛋白基因AhSFH的克隆及其响应黄曲霉菌侵染的表达特征分析[J]. 作物学报, 2025, 51(6): 1489-1500.
[15] 梁红凯, 赵苏蒙, 陆琼, 周鹏, 智慧, 刁现民, 贺强. 谷子微核心种质的构建[J]. 作物学报, 2025, 51(6): 1435-1444.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2