作物学报 ›› 2013, Vol. 39 ›› Issue (12): 2145-2153.doi: 10.3724/SP.J.1006.2013.02145
王晓波1,滕婉2,何雪2,童依平2,*
WANG Xiao-Bo1,TENG Wan2,HE Xue2,TONG Yi-Ping2,*
摘要:
从Phytozome数据库中获得包括大豆在内的12种植物的谷氨酰胺合成酶(glutamine synthetase, GS)氨基酸序列,利用MEGA5.10软件进行多序列比对、构建进化树。进化分析表明,植物GS可以分成胞质型(GS1)和质体型(GS2)两大类,GS1可进一步分成分5个亚类,包括双子叶植物为主的I、II和III亚类、低等植物类(IV)和单子叶植物类(V)。这5亚类中,第II类是豆科植物特有的一类,大豆的4个GS1 (GmGS1β1/2和GmGS1γ1/2)属于该亚类;利用qPCR在大豆盛花期分析GS1基因的组织表达特异性,结果表明不同类型GmGS1基因在表达部位和表达丰度上存在较大差异,而同一类基因之间具有相似的表达规律;4个豆科植物特有的GS1基因在大豆根瘤中都有较高的表达量,其中位于大豆第18染色体上的GmGS1β2基因表达丰度最高;利用原核表达系统体外表达GmGS1β2蛋白,诱导出分子量大小与理论预测值一致的目标蛋白,酶活性分析表明GmGS1β2可以与底物发生催化反应,具有谷氨酰胺合成酶活性,推测该基因在大豆根瘤氮素同化代谢中具有重要作用。
[1]Peterman T K, Goodman H M. The glutamine synthetase gene family of Arabidopsis thaliana: light-regulation and differential expression in leaves, roots and seeds. Mol Gen Genet, 1991, 230: 145–154[2]Taira M, Valtersson U, Burkhardt B, Ludwig R A. Arabidopsis thaliana GLN2-encoded glutamine synthetase is dual targeted to leaf mitochondria and chloroplasts. Plant Cell, 2004, 16: 2048–2058[3]Li R J, Hua W, Lu Y T. Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence. Biochem Biophys Res Commun, 2006, 342: 119–126[4]Masclaux-Daubresse C, Daniel-Vedele F, Dechorgnat J, Chardon F, Gaufichon L, Suzuki A. Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture. Ann Bot, 2011, 105: 1141–1157[5]Edwards J W, Walker E L, Coruzzi G M. Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase. Proc Natl Acad Sci USA, 1990, 87: b3459–3463[6]Bernard S M, Habash D Z. The importance of cytosolic glutamine synthetase in nitrogen assimilation and recycling. New Phytol, 2009, 182: 608–620[7]Martin A, Lee J, Kichey T, Gerentes D, Zivy M, Tatout C, Dubois F, Balliau T, Valot B, Davanture M, Terce-Laforgue T, Quillere I, Coque M, Gallais A, Gonzalez-Moro M B, Bethencourt L, Habash D Z, Lea P J, Charcosset A, Perez P, Murigneux A, Sakakibara H, Edwards K J, Hirel B. Two cytosolic glutamine synthetase isoforms of maize are specifically involved in the control of grain production. Plant Cell, 2006, 18: 3252–3274[8]Morey K J, Ortega J L, Sengupta-Gopalan C. Cytosolic glutamine synthetase in soybean is encoded by a multigene family, and the members are regulated in an organ–specific and developmental manner. Plant Physiol, 2002, 128: 182–193[9]Wang Y-F(王月福), Yu Z-W(于振文), Li S-X(李尚霞), Yu S-L(余松裂). Comparison of nitrate reductase and glutamine synthetase activities in different organs of wheat after flowering. Plant Physi Commun (植物生理学通讯), 2003, 39(3): 209–210 (in Chinese with English abstract)[10]Habash D Z, Massiah A J, Rong H L, Wallsgrove R M, Leigh R A. The role of cytosolic glutamine synthetase in wheat. Ann Appl Biol, 2001, 138: 83–89[11]Hirel B, Bertin P, Quilleré I, Bourdoncle W, Attagnant C, Dellay C, Gouy A, Cadiou S, Retailliau C, Falque M. Towards a better understanding of the genetic and physiological basis for nitrogen use efficiency in maize. Plant Physiol, 2001, 125: 1258–1270[12]Hirel B, Le Gouis J, Ney B, Gallais A. The challenge of improving nitrogen use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches. J Exp Bot, 2007, 58: 2369–2387[13]Gallais A, Hirel B. An approach to the genetics of nitrogen use efficiency in maize. J Exp Bot, 2004, 55: 295–306[14]Yamaya T, Obara M, Nakajima H, Sasaki S, Hayakawa T, Sato T. Genetic manipulation and quantitative-trait loci mapping for nitrogen recycling in rice. J Exp Bot, 2002, 53: 917–925[15]Obara M, Sato T, Sasaki S, Kashiba K, Nagano A, Nakamura I, Ebitani T, Yano M, Yamaya T. Identification and characterization of a QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice. Theor Appl Genet, 2004, 110: 1–11[16]Li X P, Zhao X Q, He X, Zhao G Y, Li B, Liu D C, Zhang A M, Zhang X Y, Tong Y P, Li Z S. Haplotype analysis of the genes encoding glutamine synthetase plastic isoforms and their association with nitrogen-use and yield-related traits in bread wheat. New Phytol, 2011, 189: 449–458[17]Masalkar P, Wallace I S, Hwang J H, and Roberts M D. Interaction of cytosolic glutamine synthetase of soybean root nodules with the C–terminal domain of the symbiosome membrane nodulin 26 aquaglyceroporin. J Biol Chem, 2010, 285: 23880–23888[18]Tamura K, Peterson D, Peterson N, Stecher G, Nei M, and Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2010, 28: 2731–2739[19]Schmutz J, Cannon S B, Schlueter J, Ma J, Mitros T, Nelson W, Hyten D L, Song Q, Thelen J J, Cheng J, Xu D, Hellsten U, May G D, Yu Y, Sakurai T, Umezawa T, Bhattacharyya M K, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang X C, Shinozaki K, Nguyen H T, Wing R A, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker R C, Jackson S A. Genome sequence of the palaeopolyploid soybean. Nature, 2010, 463: 178–183[20]Ohno S. Evolution by gene duplication. New York: Springer-Verlag, 1970[21]Hughes A L. The evolution of functionally novel proteins after gene duplication. Proc Biol Sci, 1994, 256, 119–124[22]Ortega J L, Temple S J, Sengupta–Gopalan C. Constitutive overexpression of cytosolic glutamine synthetase (GS1) gene in transgenic alfalfa demonstrates that GS1 may be regulated at the level of RNA stability and protein turnover. Plant Physiol, 2001, 126: 109–121[23]Ortega J L, Moguel–Esponda S, Potenza C, Conklin C F, Quintana A, Sengupta–Gopalan C. The 3' untranslated region of a soybean cytosolic glutamine synthetase (GS1) affects transcript stability and protein accumulation in transgenic alfalfa. Plant J, 2006, 45: 832–846[24]Ortega J L, Wilson O L, Sengupta–Gopalan C. The 5' untranslated region of the soybean cytosolic glutamine synthetase beta(1) gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants. Mol Genet Genomics, 2012, 287: 881–893[25]Miao G H, Hirel B, Marsolier M C, Ridge R W, Verma D P S: Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus. Plant Cell, 1991, 3:11–22[26]Marsolier M C, Debrosses G, Hirel B. Identification of several soybean cytosolic glutamine synthetase transcripts highly or specifically expressed in nodules: expression studies using one of the corresponding genes in transgenic lotus corniculatus. Plant Mol Biol, 1995, 27: 1–15 |
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