作物学报 ›› 2014, Vol. 40 ›› Issue (04): 636-643.doi: 10.3724/SP.J.1006.2014.00636
曾秀存1,2,刘自刚2,史鹏辉2,许耀照1,孙佳3,方彦4,杨刚2,武军艳2,孔德晶2,孙万仓2,*
ZENG Xiu-Cun1,2,LIU Zi-Gang2,SHI Peng-Hui2,XU Yao-Zhao1,SUN Jia3,FANG Yan4,YANG Gang2,WU Jun-Yan2,KONG De-Jing2,SUN Wan-Cang2,*
摘要:
超氧化物歧化酶(SOD)是一种逆境条件下清除细胞内活性氧的关键酶,而铜锌超氧化物歧化酶(Cu/Zn-SOD)是该酶系中最主要的活性氧清除剂,与植物的抗逆性关系密切。本研究根据已发表的十字花科植物Cu/Zn-SOD基因的编码区设计引物,采用RT-PCR克隆超强抗寒冬油菜陇油7号Cu/Zn-SOD的cDNA序列,该基因全长459 bp。生物信息学分析表明,该基因与甘蓝型油菜(Brassica napus) Cu/Zn-SOD基因同源性高达99%,编码1个亲水性稳定蛋白,无跨膜结构域和信号肽,具有胞质Cu/Zn-SOD超基因家族特有的序列特征和保守结构区域。半定量RT-PCR以及SOD酶活性分析表明,低温诱导条件下Cu/Zn-SOD基因差异表达,在白菜型冬油菜适应低温胁迫过程中发挥重要作用。超强抗寒冬油菜陇油6号品种低温诱导蛋白的SDS-PAGE分析以及蛋白串联质谱技术鉴定表明Cu/Zn-SOD是受低温诱导表达的抗逆基因。
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