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作物学报 ›› 2014, Vol. 40 ›› Issue (05): 924-933.doi: 10.3724/SP.J.1006.2014.00924

• 研究简报 • 上一篇    下一篇

普通菜豆基因组SSR标记开发及在豇豆和小豆中的通用性分析

陈明丽1,王兰芬1,武晶1,张晓艳2,杨广东3,王述民1,*   

  1. 1 中国农业科学院作物科学研究所,北京100081;2 青岛市农业科学研究院蔬菜所,山东青岛266100;3 黑龙江省农业科学院克山分院,黑龙江克山161606
  • 收稿日期:2013-12-10 修回日期:2014-03-04 出版日期:2014-05-12 网络出版日期:2014-03-25
  • 通讯作者: 王述民, E-mail: wangshumin@caas.cn, Tel: 010-62175628
  • 作者简介:王述民, E-mail: wangshumin@caas.cn, Tel: 010-62175628
  • 基金资助:

    本研究由中国农业科学院科技创新工程和国家科技支撑计划(2013BAD01B05-2-4)资助。

Development of Genomic SSR Markers in Common Bean and Their Transferability in Cowpea and Adzuki Bean

CHEN Ming-Li1,WANG Lan-Fen1,WU Jing1,ZHANG Xiao-Yan2,YANG Guang-Dong3,WANG Shu-Min1,*   

  1. 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Institute of Vegetables, Qingdao Academy of Agricultural Sciences, Qingdao 266100, China; 3 Keshan Branch, Heilongjiang Academy of Agricultural Sciences, Keshan 161606, China
  • Received:2013-12-10 Revised:2014-03-04 Published:2014-05-12 Published online:2014-03-25
  • About author:王述民, E-mail: wangshumin@caas.cn, Tel: 010-62175628

摘要:

分子标记具种属间通用性可提高其利用效率,并降低标记开发成本。本研究基于Roche 454超高通量测序技术获得普通菜豆基因组测序结果,共开发了560个普通菜豆基因组SSR标记。利用2份普通菜豆品种对标记进行初步筛选,有421个标记能够有效扩增。用新开发的标记分析16份豇豆和16份小豆的通用性。结果显示,185个普通菜豆基因组SSR标记在豇豆中能有效扩增,通用性比率为43.9%161SSR标记在小豆中能有效扩增,通用性比率为38.2%;在豇豆和小豆中都能获得有效扩增条带的标记共138个;并且普通菜豆基因序列SSR标记在豇豆和小豆中的通用性比率高于基因间序列SSR标记。通用性标记的多态性分析表明,豇豆和小豆的多态性比率分别为34.0%24.8%;且豇豆和小豆中基因间标记的多态性都比基因内标记的多态性高。上述通用性标记为豇豆属作物的多样性评价、连锁图谱的构建及基因定位等方面的研究提供了便利

关键词: 普通菜豆, 豇豆属, SSR标记, 通用性

Abstract:

Transferability analysis of molecular markers has significantly improved their development efficiency and reduced their development cost. A total of 560 novel SSR markers were successfully developed based on common bean genomic sequences, and 421 (75.2%) of those markers generated effective amplification bands from two accessions of the cultivated common bean. Two Vigna species, cowpea and adzuki bean were used to test the transferability and polymorphism of new genomic markers. The results indicated that the transferability rate of common bean genomic-SSR in cowpea and adzuki bean was 43.9% and 38.2%, and the ratio of polymorphism SSR markers in the crops was 34.0% and 24.8%, respectively. A total of 138 common bean genomic-SSR primers were detected to be highly transferable between two species of Vigna. In addition, the diversity of transferability gene-SSR markers was higher for cowpea and adzuki bean than that of intergenic regions-SSR. These transferable markers are useful for further genetic diversity analysis, genetic linkage map construction, quantitative trait loci annotation, genetic evolution and marker-assisted selection in Vigna species because of their multi-allelic nature, reproducibility, co-dominant inheritance, high abundance in genome.

Key words: Common bean, Genus Vigna, SSR markers, Transferability

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