作物学报 ›› 2014, Vol. 40 ›› Issue (05): 934-941.doi: 10.3724/SP.J.1006.2014.00934
陈娜1,潘丽娟1,迟晓元1,2,陈明娜1,王通1,王冕1,杨珍1,胡冬青3,王道远4,禹山林1,*
HEN Na1,PAN Li-Juan1,CHI Xiao-Yuan1,2,CHEN Ming-Na1,WANG Tong1,WANG Mian1,YANG Zhen1,HU Dong-Qing3,WANG Dao-Yuan4,YU Shan-Lin1,*
摘要:
以花生品种花育33为试材, 根据cDNA文库中已知的果糖-1,6-二磷酸醛缩酶 (fructose-1,6-bisphosphate aldolase, FBA) 基因全长序列设计引物, 通过RT-PCR克隆到该基因, 命名为AhFBA1。AhFBA1全长为1489 bp, 开放阅读框为1200 bp, 编码400个氨基酸。预测该基因编码的蛋白含有Glycolytic保守结构域, 可能定位于叶绿体中。蛋白序列比对和进化树分析表明, 花生与大豆(Glycine max)、苜蓿(Medicago truncatula)、鹰嘴豆(Cicer arietinum)和菜豆(Phaseolus vulgaris)等豆科植物中的FBA序列相似性最高, 亲缘关系最近。荧光定量PCR结果显示, 在高盐和干旱胁迫下, AhFBA1在花生叶和根中的表达均受明显诱导, 说明该基因可能参与花生对高盐和干旱胁迫的适应性调控; AhFBA1在花生根和叶中均受ABA的明显诱导, 说明该基因对花生非生物胁迫的调控可能是依赖ABA的。
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