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作物学报 ›› 2016, Vol. 42 ›› Issue (05): 714-724.doi: 10.3724/SP.J.1006.2016.00714

• 耕作栽培·生理生化 • 上一篇    下一篇

可溶性寡糖和小分子的热激蛋白与杂交水稻种子成熟过程中发芽能力及种子活力相关

朱丽伟1,2,**,曹栋栋3,**,付玉营1,胡琦娟1,利站1,关亚静1,胡伟民1,胡晋1,*   

  1. 1浙江大学农业与生物技术学院种子科学中心,浙江杭州310058;2贵州师范大学荞麦产业技术研究中心,贵州贵阳550001;3浙江农科种业有限公司,浙江杭州310058
  • 收稿日期:2015-08-14 修回日期:2016-03-02 出版日期:2016-05-12 网络出版日期:2016-03-11
  • 通讯作者: 胡晋, E-mail: jhu@zju.edu.cn**同等贡献(Contributed equally to this work)
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(201203052), 浙江省科技厅项目(2013C02005, 2013C32023), 浙江省自然科学基金项目(LZ14C130002, LY15C130002)和江苏省现代作物生产协同创新中心项目资助。

Soluble Oligosaccharide and Small Heat Shock Protein Correlated with Seed Germination and Vigor during Hybrid Rice Seed Maturation

ZHU Li-Wei1,2,**,CAO Dong-Dong3,**,FU Yu-Ying1,HU Qi-Juan1,LI Zhan1,GUAN Ya-Jing1,HU Wei-Min1,HU Jin1,*   

  1. 1Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China; 3Zhejiang Agriculture Science Limited Company, Hangzhou310058, China
  • Received:2015-08-14 Revised:2016-03-02 Published:2016-05-12 Published online:2016-03-11
  • Contact: Hu Jin, E-mail: jhu@zju.edu.cn**同等贡献(Contributed equally to this work)
  • Supported by:

    This study was supported by the China Special Fund for Agro-Scientific Research in the Public Interest (201203052), the Subject Fund of ZhejiangProvince Science and Technology Hall (2013C02005, 2013C32023), the Natural Sciences Fund of ZhejiangProvince (LZ14C130002, LY15C130002), and JiangsuCollaborative Innovation Center for Modern Crop Production.

摘要:

系统研究了不同成熟期Y两优689杂交水稻种子的活力、可溶性寡糖含量和小分子热激蛋白表达量的变化。运用模糊隶属函数法综合分析种子的发芽率、发芽势、发芽指数、活力指数、平均发芽时间、根数、根长、苗高、苗重发现,授粉后19~34d的种子生活力与活力均较高。种子成熟过程中含水量呈下降趋势,授粉后10~22 d,种子含水量从48.2%下降至23.9%;鲜种子棉子糖与果糖含量分别呈上升和下降趋势;水苏糖和葡萄糖呈先上升后下降趋势。授粉后10~19 d,小分子热激蛋白64SHsp18.0Os03g0267200基因的表达量均呈上升趋势,此后相对表达量下降。水分、棉子糖、水苏糖和蔗糖等物质含量,鲜种子及干种子的千粒重,以及64SHsp18.0Os03g0267200基因的相对表达量均与种子发芽密切相关。

关键词: 杂交水稻, 种子, 成熟, 小热激蛋白, 寡糖, 活力

Abstract:

To study the relationship between seed soluble oligosaccharide, small heat shock protein (sHSPs) and seed vigor, we systematically studied their changes during hybrid rice seed maturity.The mean fuzzy subordinate function values of germination percentage, germination energy, germination index, vigor index and mean germination time, root number, root length, shoot length and seedling weight were analyzed. The resultsindicated thatseeds harvesting at 19 to 34 days after pollination (DAP) had a higher viability and vigor. The seed moisture content had a decline trend, with a decrease from 48.2% at 10 DAP to 23.9% at 22 DAP. The raffinose content increased significantly, the fructose content kept decreasing, the contents of stachyose and glucose significantly increased firstly and then decreased during seed development. The relative expression levels of 64SHsp18.0 and Os03g0267200 genes increased significantly from 10 to 19 DAP, and then decreased. The moisture content,the contents of raffinose,stachyose andsucrose, thousand seed weight of fresh seeds and dry seeds,relative transcriptions of 64SHsp18.0 and Os03g0267200 genes were all significantly correlated with seed germination during seed maturity.

Key words: Hybrid rice, Seed, Maturity, sHSPs, Oligosaccharide, Vigor

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