作物学报 ›› 2018, Vol. 44 ›› Issue (04): 505-511.doi: 10.3724/SP.J.1006.2018.00505
张宏军1(), 宿振起2,3, 柏贵华3,4, 张旭5, 马鸿翔5, 李腾1, 邓云6, 买春艳7, 于立强8, 刘宏伟1, 杨丽1, 李洪杰1,*(), 周阳1,*()
Hong-Jun ZHANG1(), Zhen-Qi SU2,3, Gui-Hua BAI3,4, Xu ZHANG5, Hong-Xiang MA5, Teng LI1, Yun DENG6, Chun-Yan MAI7, Li-Qiang YU8, Hong-Wei LIU1, Li YANG1, Hong-Jie LI1,*(), Yang ZHOU1,*()
摘要:
赤霉病已上升为黄淮冬麦区的主要病害, 提高小麦品种对赤霉病的抗性成为该麦区主要的育种目标之一。宁麦9号、生选6号、建阳798、建阳84、苏麦3号和宁麦13均携带Fhb1基因, 对赤霉病表现中抗水平以上。本研究以这6个品种(系)为供体, 分别与高感赤霉病的周麦16矮败小麦近等基因系杂交和回交, 构建6个回交群体。利用Fhb1基因的KASP标记在回交后代中进行基因型分析, 分别选择携带和不携带Fhb1基因的可育株, 对后代株系进行单花滴注接种鉴定和田间病圃自然鉴定。回交后代携带Fhb1家系整体抗性达到中感, 比不携带Fhb1家系的平均病小穗数低4.2 (P < 0.01), 平均病情指数低4.0, 比轮回亲本周麦16的平均病小穗数和病情指数分别低8.1 (P < 0.01)和28.4 (P < 0.01)。不同供体品种(系)回交后代在赤霉病抗性上表现出明显差异, 以生选6号为供体的回交后代家系抗性表现最好。本研究表明, 利用Fhb1基因分子标记辅助选择技术能够有效地提高黄淮冬麦区小麦品种的赤霉病抗性水平。
[1] | Yin Y, Liu X, Li B, Ma Z.Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F. graminearum collected from wheat in China. Phytopathology, 2009, 99: 487-497 |
[2] | 程顺和, 张勇, 别同德, 高德荣, 张伯桥. 中国小麦赤霉病的危害及抗性遗传改良. 江苏农业学报, 2012, 28: 938-942 |
Cheng S H, Zhang Y, Bie T D, Gao D R, Zhang B Q.Damage of wheat Fusarium head blight epidemics and genetic improvement of wheat for scab resistance in China.Jiangsu J Agric Sci, 2012, 28: 938-942 (in Chinese with English abstract) | |
[3] | 刘新月, 裴磊, 卫云宗, 张正斌, 高辉明, 徐萍. 气温变化背景下中国黄淮旱地冬小麦农艺性状的变化特征——以山西临汾为例. 中国农业科学, 2015, 48: 1942-1954 |
Liu X Y, Pei L, Wei Y Z, Zhang Z B, Gao H M, Xu P.Agronomic traits variation analysis of Huanghuai dryland winter wheat under temperature change background in China: taking Linfen, Shanxi as an example.Sci Agric Sin, 2015, 48: 1942-1954 (in Chinese with English abstract) | |
[4] | Anderson J A, Stack R W, Liu S, Waldron B L, Fjeld A D, Coyne C, Moreno-Sevilla B, Fetch J M, Song Q J, Cregan P B, Frohberg R C.DNA markers for Fusarium head blight resistance QTLs in two wheat populations.Theor Appl Genet, 2001, 102: 1164-1168 |
[5] | Cuthbert P A, Somers D J, Thomas J, Cloutier S, Brulé-Babel A.Fine mapping Fhb1, a major gene controlling Fusarium head blight resistance in bread wheat(Triticum aestivum L.). Theor Appl Genet, 2006, 112: 1465-1472 |
[6] | Cuthbert P A, Somers D J, Brulé-Babel A.Mapping of Fhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat(Triticum aestivum L.). Theor Appl Genet, 2007, 114: 429-437 |
[7] | Qi L L, Pumphre M O, Friebe B, Chen P D, Gill B S.Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat. Theor Appl Genet, 2008, 117: 1155-1166 |
[8] | Guo J, Zhang X, Hou Y, Cai J, Shen X, Zhou T, Xu H, Ohm H W, Wang H, Li A, Han F, Wang H, Kong L.High-density mapping of the major FHB resistance geneFhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theor Appl Genet, 2015, 128: 2301-2316 |
[9] | Xue S, Li G, Jia H, Xu F, Lin F, Tang M, Wang Y, An X, Xu H, Zhang L, Kong Z, Ma Z.Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2010, 121: 147-156 |
[10] | Xue S, Xu F, Tang M, Zhou Y, Li G, An X, Lin F, Xu H, Jia H, Zhang L, Kong Z, Ma Z.Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat ( Triticum aestivum L.). Theor Appl Genet, 2011, 123: 1055-1063 |
[11] | 刘易科, 佟汉文, 朱展望, 陈泠, 邹娟, 张宇庆, 焦春海, 高春保. 小麦赤霉病抗性改良研究进展. 麦类作物学报, 2016, 36: 51-57 |
Liu Y K, Tong H W, Zhu Z W, Chen L, Zou J, Zhang Y Q, Jiao C H, Gao B C.Review on improvement of Fusarium head blight resistance in wheat.J Triticeae Crops, 2016, 36: 51-57 (in Chinese with English abstract) | |
[12] | Brown-Guedira G, Griffey C, Kolb F, McKendry A, Murphy J, Sanford D. Breeding FHB-resistant soft winter wheat: Progress and prospects.Cereal Res Commun, 2008, 36: 31-35 |
[13] | Bernardo A, Bai G, Yu J, Kolb F, Bockus W, Dong Y.Registration of near-isogenic winter wheat germplasm contrasting in Fhb1 for Fusarium head blight resistance. J Plant Regist, 2013, 8: 106-108 |
[14] | Randhawa H S, Asif M, Pozniak C, Clarke J M, Graf R J, Fox S L, Humphreys D G, Knox R E, DePauw R M, Singh A K, Cuthbert R D, Hucl P, Spaner D. Application of molecular markers to wheat breeding in Canada.Plant Breed, 2013, 132: 458-471 |
[15] | Xie G Q, Zhang M C, Chakraborty S, Liu C J.The effect of 3BS locus of Sumai 3 on Fusarium head blight resistance in Australian wheats.Aust J Exp Agric, 2007, 47: 603-607 |
[16] | Oda S.Advances in Wheat Genetics:From Genome to Field. Berlin Heidelberg: Springer-Verlag Press, 2015. pp 311-318 |
[17] | 张旭, 任丽娟, 谭秀云, 藏宇辉, 马鸿翔, 秦浚川, 陆维忠. 利用分子标记研究小麦遗传群体的赤霉病抗性鉴定方法. 南京大学学报(自然科学), 2005, 41: 125-132 |
Zhang X, Ren L J, Tan X Y, Zang Y H, Ma H X, Qin J C, Lu W Z.Evaluation method of wheat Fusarium head blight resistance for genetic population based on molecular markers.J Nanjing Univ(Nat Sci), 2005, 41: 125-132 (in Chinese with English abstract) | |
[18] | 中华人民共和国农业部. 小麦抗病虫性评价技术规范——第4部分: 小麦抗赤霉病评价技术规范, 2007. pp 1-6 |
Ministry of Agriculture of the People’s Republic of China. Rules for Resistance Evaluation of Wheat to Diseases and Insect Pests—Part 4: Rules for Resistance Evaluation of Wheat to Wheat Scab (Fusarium graminearum Schwabe [Teleomorph Gibberella zeae (Schwein) Petch]), 2007. pp 1-6 (in Chinese) | |
[19] | Rasheed A, Wen W, Gao F, Zhai S, Jin H, Liu J, Guo Q, Zhang Y, Dreisigacker S, Xia X, He Z.Development and validation of KASP assays for genes underpinning key economic traits in bread wheat.Theor Appl Genet, 2016, 129: 1843-1860 |
[20] | 刘秉华, 杨丽. “矮败”小麦的选育. 中国农业科学, 1990, 23(3): 86-87 |
Liu B H, Yang L.Breeding of dwarfing and male-sterile wheat.Sci Agric Sin, 1990, 23(3): 86-87 (in Chinese) | |
[21] | 刘秉华, 杨丽. “矮败”小麦的选育及利用前景. 科学通报, 1991, 36: 306-308 |
Liu B H, Yang L.Breeding and application of dwarfing and male-sterile wheat. Chin Sci Bull, 1991, 36: 306-308 (in Chinese) | |
[22] | 陆维忠. 小麦赤霉病抗性分子标记的筛选及其利用. 江苏农业学报, 2011, 27: 243-249 |
Lu W Z.Screening and application of molecular markers linked to wheat scab resistance.Jiangsu J Agric Sci, 2011, 27: 243-249 (in Chinese with English abstract) | |
[23] | Liu S, Pumphrey M, Gill B, Trick H, Zhang J, Dolezel J, Chalhoub B, Anderson J.Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat. Cereal Res Commun, 2008, 36: 195-201 |
[24] | Zhou W C, Kolb F L, Bai G H, Domier L L, Yao J B.Effect of individual Sumai 3 chromosomes on resistance to scab spread within spikes and deoxynivalenol accumulation within kernels in wheat. Hereditas, 2002, 137: 81-89 |
[25] | Pumphrey M O, Bernardo R, Anderson J A.Validating the Fhb1 QTL for Fusarium head blight resistance in near-isogenic wheat lines developed from breeding populations. Crop Sci, 2007, 47: 200-206 |
[26] | 陆维忠, 马鸿翔. 高产抗赤霉病小麦新品种生选6号的选育. 江苏农业科学, 2010, 38(3): 153-154 |
Lu W Z, Ma H X.Breeding of wheat cultivar Shengxuan 6 for high-resistant Fusarium head blight.Jiangsu Agric Sci, 2010, 38(3): 153-154 (in Chinese) | |
[27] | 姚金保, 陆维忠. 小麦新品种生选6号的丰产稳产性和赤霉病抗性分析. 麦类作物学报, 2012, 32: 793-798 |
Yao J B, Lu W Z.Analysis of yield potential, yield stability and resistance to Fusarium head blight of new wheat cultivar Shengxuan 6.J Triticeae Crops, 2012, 32: 793-798 (in Chinese with English abstract) | |
[28] | 张旭, 姜朋, 叶人元, 吴磊, 张瑜, 马鸿翔. 宁麦9号及其衍生品种的赤霉病抗性分析与抗性溯源. 分子植物育种, 2017, 15: 1053-1060 |
Zhang X, Jiang P, Ye R Y, Wu L, Zhang Y, Ma H X.Evaluation and source tracing of resistance to Fusarium head blight in wheat variety Ningmai 9 and its derivatives.Mol Plant Breed, 2017, 15: 1053-1060 (in Chinese with English abstract) |
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