欢迎访问作物学报，今天是
作物学报 ›› 2020, Vol. 46 ›› Issue (5): 645-660.doi: 10.3724/SP.J.1006.2020.94133
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
王晓阳1,王丽媛1,潘兆娥1,何守朴1,王骁1,龚文芳1,3,*(
),杜雄明1,2,*()
Xiao-Yang WANG1,Li-Yuan WANG1,Zhao-E PAN1,Shou-Pu HE1,Xiao WANG1,Wen-Fang GONG1,3,*(),Xiong-Ming DU1,2,*()
摘要：
棉花纤维是重要的天然纺织材料, 是最长的单细胞, 是研究纤维发育的良好材料。本研究以亚洲棉短绒突变体(FZ)及其野生型(fz)为材料, 结合扫描电镜、石蜡切片、RNA-seq技术, 解析棉花短绒起始的可能机制。与野生型(fz)的0DPA时期胚珠相比, 突变体的胚珠在该时期仅有少量的纤维起始。在+3DPA时, 突变体没有短绒细胞起始, 仅有长纤维细胞, 而野生型有大量的短纤维细胞和长纤维细胞。对这2个材料的0DPA、+3DPA、+5DPA和+8DPA胚珠差异基因分析结果显示, 在短绒突变体(FZ)和野生型(fz)的4个纤维发育时期共挖掘出3780个差异表达基因, 其中0DPA时差异基因数目最少, 随着胚珠发育时间的延长, 差异基因的数目逐渐增加。KEGG分析发现这些基因主要参与蜡质、角质生物合成, 以及苯丙烷代谢和植物信号传导过程。共表达趋势分析显示, 在突变体+3DPA上调的差异基因中, 参与离子结合、MAPK级联反应、氧化还原活性和转录调控的基因表达受到正影响(表达水平提高), 造成突变体短绒纤维不能正常起始。这些结果描述了二倍体亚洲棉短绒起始的动态变化, 可为进一步研究棉纤维发育提供参考。
| [1] |
Lee J J, Woodward A W, Chen Z J . Gene expression changes and early events in cotton fibre development. Ann Bot, 2007,100:1391-1401.
doi: 10.1093/aob/mcm232 pmid: 17905721 |
| [2] |
Kim H J, Triplett B A . Cotton fiber growth in planta and in vitro. Models for plant cell elongation and cell wall biogenesis. Plant Physiol, 2001,127:1361-1366.
pmid: 11743074 |
| [3] |
Turley R B, Kloth R H . Identification of a third fuzzless seed locus in upland cotton ( Gossypium hirsutum L.). J Heredity, 2002,93:359-364.
doi: 10.1093/jhered/93.5.359 |
| [4] |
Arpat A B, Waugh M, Sullivan J P, Gonzales M, Frisch D, Main D, Wood T, Leslie A, Wing R A, Wilkins T A . Functional genomics of cell elongation in developing cotton fibers. Plant Mol Biol, 2004,54:911-929.
doi: 10.1007/s11103-004-0392-y |
| [5] |
Shi Y H, Zhu S W, Mao X Z, Feng J X, Qin Y M, Zhang L, Cheng J, Wei L P . Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. Plant Cell, 2006,18:651-664.
doi: 10.1105/tpc.105.040303 pmid: 16461577 |
| [6] |
Yang S S, Cheung F, Lee J J, Ha M, Wei N E, Sze S H, Stelly D M, Thaxton P, Triplett B, Town C D . Accumulation of genome-specific transcripts, transcription factors and phytohormonal regulators during early stages of fiber cell development in allotetraploid cotton. Plant J, 2006,47:761-775.
doi: 10.1111/j.1365-313X.2006.02829.x pmid: 16889650 |
| [7] |
Wang M J, Tu L L, Yuan D J, Zhu D, Shen C, Li J Y, Liu F Y, Pei L L, Wang P C, Zhao G N, Ye Z X, Huang H, Yan F L, Ma Y Z, Zhang L, Liu M, You J Q, Yang Y C, Liu Z P, Huang F, Li B Q, Qiu P, Zhang Q H, Zhu L F, Jin S X, Yang X Y, Min L, Li G L, Chen L L, Zheng H K, Lindsey K, Lin Z X, Udall J A, Zhang X L . Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense. Nat Genet, 2018,51:224-229.
doi: 10.1038/s41588-018-0282-x pmid: 30510239 |
| [8] |
Du X, Huang G, He S, Yang Z, Sun G, Ma X, Li N, Zhang X, Sun J, Liu M, Jia Y, Pan Z, Gong W, Liu Z, Zhu H, Ma L, Liu F, Yang D, Wang F, Fan W, Gong Q, Peng Z, Wang L, Wang X, Xu S, Shang H, Lu C, Zheng H, Huang S, Lin T, Zhu Y, Li F . Resequencing of 243 diploid cotton accessions based on an updated a genome identifies the genetic basis of key agronomic traits. Nat Genet, 2018,50:796-802.
doi: 10.1038/s41588-018-0116-x pmid: 29736014 |
| [9] |
Zhang T, Hu Y, Jiang W, Fang L, Guan X, Chen J, Zhang J, Saski C A, Scheffler B E, Stelly D M, Hulse-Kemp A M, Wan Q, Liu B, Liu C, Wang S, Pan M, Wang Y, Wang D, Ye W, Chang L, Zhang W, Song Q, Kirkbride R C, Chen X, Dennis E, Llewellyn D J, Peterson D G, Thaxton P, Jones D C, Wang Q, Xu X, Zhang H, Wu H, Zhou L, Mei G, Tian Y, Xiang D, Li X, Zuo Q, Tao S, Chen L, Liu Y, Li J, Lin Y, Hui Z, Cao L, Cai C, Zhu X, Jiang Z, Zhou B, Guo W, Li R, Chen Z J . Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotechnol, 2015,33:531-537.
doi: 10.1038/nbt.3207 pmid: 25893781 |
| [10] |
Islam M S, Fang D D, Thyssen G N, Delhom C D, Liu Y L, Kim H J . Comparative fiber property and transcriptome analyses reveal key genes potentially related to high fiber strength in cotton (Gossypium hirsutum L.) line MD52ne. BMC Plant Biol, 2016,16:1-19.
doi: 10.1186/s12870-015-0700-5 pmid: 26728271 |
| [11] |
Hande A S, Katageri I S, Jadhav M P, Adiger S, Gamanagatti S, Padmalatha K V, Dhandapani G, Kanakachari M, Kumar P A, Reddy V S . Transcript profiling of genes expressed during fibre development in diploid cotton ( Gossypium arboreum L.). BMC Genomics, 2017,18:675.
doi: 10.1186/s12864-017-4066-y pmid: 28859611 |
| [12] |
Trapnell C, Pachter L, Salzberg S L . TopHat: discovering splice junctions with RNA-Seq. Bioinformatics, 2009,25:1105-1111.
doi: 10.1093/bioinformatics/btp120 pmid: 19289445 |
| [13] |
Cole T, Adam R, Loyal G, Geo P, Daehwan K, Kelley D R, Harold P, Salzberg S L, Rinn J L, Lior P . Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protocols, 2012,7:562-578.
doi: 10.1038/nprot.2012.016 pmid: 22383036 |
| [14] |
Ogata H, Goto S, Sato K, Fujibuchi W, Bono H, Kanehisa M . KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res, 1999,27:29-34.
doi: 10.1093/nar/27.1.29 pmid: 9847135 |
| [15] |
Minoru K, Susumu G, Yoko S, Masayuki K, Miho F, Mao T . Data, information, knowledge and principle: back to metabolism in KEGG. Nucleic Acids Res, 2014,42:199-205.
doi: 10.1093/nar/gkt1076 pmid: 24214961 |
| [16] |
Livak K J, Schmittgen T D . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 2001,25:402-408.
doi: 10.1006/meth.2001.1262 pmid: 11846609 |
| [17] |
Wan Q, Guan X Y, Yang N N, Wu H T, Pan M Q . Small interfering RNAs from bidirectional transcripts of GhMML3_A12 regulate cotton fiber development. New Phytol, 2016,210:1298-1310.
doi: 10.1111/nph.13860 pmid: 26832840 |
| [18] |
Wu H T, Tian Y, Wan Q, Fang L, Guan X Y, Chen J D . Genetics and evolution of MIXTA genes regulating cotton lint fiber development. New Phytol, 2018,217:883-895.
doi: 10.1111/nph.14844 pmid: 29034968 |
| [19] |
Du S J, Dong C J, Zhang B, Lai T F, Du X M, Liu J Y . Comparative proteomic analysis reveals differentially expressed proteins correlated with fuzz fiber initiation in diploid cotton (Gossypium arboreum L.). J Proteomics, 2013,82:113-129.
doi: 10.1016/j.jprot.2013.02.020 pmid: 23474080 |
| [20] |
Daviere J M, Achard P . A pivotal role of DELLAs in regulating multiple hormone signals. Mol Plant, 2016,9:10-20.
doi: 10.1016/j.molp.2015.09.011 pmid: 26415696 |
| [21] |
Chen Z J, Guan X Y . Auxin boost for cotton. Nat Biotechnol, 2011, 29.
doi: 10.1038/nbt.1858 pmid: 21552241 |
| [22] |
Xiao G H, He P, Zhao P, Liu H, Zhang L, Pang C, Yu J . Genome-wide identification of the GhARF gene family reveals that GhARF2 and GhARF18 are involved in cotton fibre cell initiation. J Exp Bot, 2018,69:4323-4337.
doi: 10.1093/jxb/ery219 pmid: 29897556 |
| [23] |
Han , X Cloning and expression analysis of novel Aux/IAA family genes in Gossypium hirsutum. Genes, 2012,503:83-91.
doi: 10.1016/j.gene.2012.03.069 pmid: 22575728 |
| [24] | Gilbert M K, Bland J M, Shockey J M, Cao H, Hinchliffe D J, Fang D D, Naoumkina M . A transcript profiling approach reveals an abscisic acid-specific glycosyltransferase (UGT73C14) induced in developing fiber of ligon lintless-2 mutant of cotton ( Gossypium hirsutum L.). PLoS One, 2013,8. |
| [25] |
Zhang D Y, Zhang T Z, Guo W Z . Effect of H2O2 on fiber initiation using fiber retardation initiation mutants in cotton (Gossypium hirsutum). J Plant Physiol, 2010,167:393-399.
doi: 10.1016/j.jplph.2009.10.005 pmid: 19931935 |
| [26] |
Taliercio E W, Boykin D . Analysis of gene expression in cotton fiber initials. BMC Plant Biol, 2007,7:22.
doi: 10.1186/1471-2229-7-22 pmid: 17506877 |
| [27] |
Kudla J, Batistic O, Hashimoto K . Calcium signals: the lead currency of plant information processing. Plant Cell, 2010,22:541-563.
doi: 10.1105/tpc.109.072686 pmid: 20354197 |
| [28] |
Steinhorst L, Mähs A, Ischebeck T, Zhang C X, Zhang X X, Arendt S, Schültke S, Heilmann I, Kudla J . Vacuolar CBL- CIPK12 Ca 2+-sensor-kinase complexes are required for polarized pollen tube growth . Curr Biol, 2015,25:1475-1482.
doi: 10.1016/j.cub.2015.03.053 pmid: 25936548 |
| [1] | 刘迪, 黎瑞源, 石茂竹, 李洪有, 陈庆富, 石桃雄. 苦荞半矮秆突变体sd3的表型鉴定及转录组分析[J]. 作物学报, 2026, 52(1): 316-328. |
| [2] | 王青, 王伊秀, 李越男, 吕永辉, 张海波, 刘娜, 程红艳. 高、低Cd积累小麦对Cd胁迫的转录组学响应差异[J]. 作物学报, 2025, 51(5): 1230-1247. |
| [3] | 张金泽, 周庆国, 杨旭, 王倩, 肖莉晶, 金海润, 欧阳青静, 余坤江, 田恩堂. 芥菜型油菜响应菌核病侵染表达特性与高抗性关联基因分析[J]. 作物学报, 2025, 51(3): 621-631. |
| [4] | 许睿, 何妙华, 王昊, 李卫, 任杰, 夏志强. 基于空间转录组技术解析大豆种胚对X射线辐射的响应机制[J]. 作物学报, 2025, 51(12): 3121-3132. |
| [5] | 赵慧霞, 郭彦丽, 郑渝泠, 何棱, 陈锐, 王珊珊, 曾长立, 邹珺, 沈金雄, 傅廷栋, 刘小云, 万何平. 甘蓝型油菜响应碱胁迫的基因表达差异分析[J]. 作物学报, 2025, 51(11): 3105-3118. |
| [6] | 王瑞, 张福耀, 詹鹏杰, 楚建强, 晋敏姗, 赵威军, 程庆军. 基于RNA-Seq筛选高粱低氮胁迫相关候选基因[J]. 作物学报, 2024, 50(3): 669-685. |
| [7] | 陈天, 李昱樱, 荣二花, 吴玉香. 棉属人工异源四倍体后代性状鉴定及花器转录组学分析[J]. 作物学报, 2024, 50(2): 325-339. |
| [8] | 李艳, 方宇辉, 王永霞, 彭超军, 华夏, 齐学礼, 胡琳, 许为钢. 不同磷胁迫处理转OsPHR2小麦的转录组学分析[J]. 作物学报, 2024, 50(2): 340-353. |
| [9] | 张锦辉, 肖姿仪, 李旭华, 张明, 贾春兰, 潘振远, 邱法展. 玉米突变体caspl2b2的耐盐特性评价及转录组分析[J]. 作物学报, 2024, 50(12): 3144-3154. |
| [10] | 王珍, 张晓莉, 刘淼, 姚梦楠, 孟晓静, 曲存民, 卢坤, 李加纳, 梁颖. 甘蓝型油菜BnMAPK1超量表达及中油821的转录差异表达分析[J]. 作物学报, 2023, 49(3): 856-868. |
| [11] | 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118. |
| [12] | 杨亚杰, 李昱樱, 申状状, 陈天, 荣二花, 吴玉香. 草棉不同倍性材料叶片转录组差异表达分析[J]. 作物学报, 2022, 48(11): 2733-2748. |
| [13] | 黄文功, 姜卫东, 姚玉波, 宋喜霞, 刘岩, 陈思, 赵东升, 吴广文, 袁红梅, 任传英, 孙中义, 吴建忠, 康庆华. 亚麻响应低钾胁迫转录谱分析[J]. 作物学报, 2021, 47(6): 1070-1081. |
| [14] | 王瑞莉, 王刘艳, 雷维, 吴家怡, 史红松, 李晨阳, 唐章林, 李加纳, 周清元, 崔翠. 结合RNA-seq分析和QTL定位筛选甘蓝型油菜萌发期与铝毒胁迫相关的候选基因[J]. 作物学报, 2021, 47(12): 2407-2422. |
| [15] | 任蒙蒙, 张红伟, 王建华, 王国英, 郑军. 玉米耐深播主效QTL qMES20-10的精细定位及差异表达基因分析[J]. 作物学报, 2020, 46(7): 1016-1024. |
|
||
