作物学报 ›› 2023, Vol. 49 ›› Issue (7): 1829-1842.doi: 10.3724/SP.J.1006.2023.24188
唐玉凤(), 姚敏, 何昕, 官梅, 刘忠松, 官春云, 钱论文*(
)
TANG Yu-Feng(), YAO Min, HE Xin, GUAN Mei, LIU Zhong-Song, GUAN Chun-Yun, QIAN Lun-Wen*(
)
摘要:
叶绿素是植物进行光合作用的一类绿色色素, 对植物生长有着直接的影响。本研究利用生物信息学方法在全基因组水平上对甘蓝型油菜(Brassica napus)、白菜(Brassica rapa)、甘蓝(Brassica oleracea)和拟南芥(Arabidopsis thaliana)中滞绿基因(STAY-GREEN, SGR)家族成员进行分析发现, 28个SGR基因大多数包含4个外显子, 编码碱性蛋白。染色体定位和共线性分析显示, 甘蓝型油菜SGR基因家族成员中不存在串联复制, SGR基因家族成员之间具有线性关系, 高度同源, 且在进化过程中非常保守。此外, 利用60K SNP对203份半冬性甘蓝型油菜自交系的叶绿素含量进行全基因组关联分析(Genome-wide association study, GWAS), 检测到2个单体型区域Chr.A01: 6,193,165~ 6,317,757 bp和Chr.C01: 9,059,861~9,906,618 bp携带的BnaSGR1a-A01和BnaSGR1-C01与叶绿素含量显著相关。同时, 结合50份半冬性甘蓝型油菜重测序数据, 对这2个单体型进行进区域关联分析, 检测到1个SNP定位在BnaSGR1a-A01的外显子2区域, 并与叶绿素含量显著关联。共表达网络分析结果验证了BnaSGR1a-A01与BnaSGR2-A03直接相连, 与BnaSGR1-C01、BnaSGR1-A08、BnaSGR2-C03、BnaSGR1-C07、BnaSGRL-C06、BnaSGRL-A10等基因间接相连形成了一个网络体系, 共同调节叶绿素含量。T2拟南芥转BnaSGR1a-A01基因超表达植株的叶绿素a、叶绿素b和总的叶绿素含量相比野生型显著降低, 表明BnaSGR1调控叶绿素降解。本研究为油菜SGR基因的功能研究和利用奠定了基础。
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