欢迎访问作物学报，今天是
作物学报 ›› 2023, Vol. 49 ›› Issue (7): 1843-1859.doi: 10.3724/SP.J.1006.2023.24173
王让剑1,2,*(
), 杨军1,2, 张力岚1,2, 高香凤1,2
WANG Rang-Jian1,2,*(), YANG Jun1,2, ZHANG Li-Lan1,2, GAO Xiang-Feng1,2
摘要：
香叶醇是茶树中含有的一种重要的挥发性单萜醇, 在茶树与环境互作中起重要作用, 也是茶叶中关键呈香成分之一, 茶树新梢中的香叶醇主要以香叶醇樱草糖苷形式存在。发掘香叶醇樱草糖苷含量性状显著关联的SNP位点与候选基因, 对香叶醇樱草糖苷含量的遗传调控机制研究与茶树遗传改良具有重要意义。以169个茶树种质为研究对象, 连续3年对其新梢香叶醇樱草糖苷含量进行鉴定, 基于SLAF简化基因组测序技术开发SNP标记, 然后利用一般线性模型(GLM)对茶树新梢香叶醇樱草糖苷含量性状进行GWAS分析, 发掘该性状显著关联的SNP位点与候选基因, 最后对极端含量材料间的候选基因编码区碱基差异及其上游顺式作用元件进行分析。结果表明, 参试群体在3个环境下香叶醇樱草糖苷含量变异系数范围为77.6%~81.8%, 广义遗传力为62.6%。香叶醇樱草糖苷含量性状在基因型、环境间均呈现显著差异, 变异受遗传影响为主。3个环境下共检测到340个SNP与香叶醇樱草糖苷含量显著相关, 其中2个环境下重复检测到65个SNP。基于参考基因组与连锁不平衡衰减距离, 获得重复检测到的SNP两侧各100 kb范围内的基因共88个, 包含信号蛋白、激酶、磷酸酶、离子转运蛋白、转录因子、热激蛋白、激素相关蛋白、抗性蛋白、萜类代谢酶、糖基转移酶、糖苷酶, 从中初筛出10个与香叶醇樱草糖苷含量相关的候选基因。极端含量材料之间的候选基因编码区序列均存在SNP非同义突变, 多数候选基因上游2 kb区域存在不同数量的与环境胁迫和激素相关的顺式作用元件。本研究为阐明茶新梢中香叶醇樱草糖苷含量的遗传调控机制提供了新的视角, 为分子标记辅助选择茶树新品种提供了标记与基因资源。
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