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藜麦RopGEF家族基因的鉴定及表达模式分析

景秀清1,2,3,*,蔡永朵1,邓宁1,赵晓东1,2,翟飞红1,2,曾群4   

  1. 1 太原师范学院生物科学与技术学院, 山西晋中030619; 2 太原师范学院汾河流域地表过程与资源生态安全山西省重点实验室, 山西晋中030619; 3 山西大学生命科学学院, 山西太原030006; 4 山西中医药大学基础医学院, 山西晋中030619
  • 收稿日期:2025-07-22 修回日期:2025-10-30 接受日期:2025-10-30 网络出版日期:2025-11-10
  • 基金资助:
    本研究由山西省基础研究计划(自由探索类)项目(202203021212186), 太原师范学院2024年度研究生教育创新项目(SYYJSYC-2431)和太原师范学院大学生创新创业训练项目(CXCY25104)资助。

Identification and expression pattern analysis of CqRopGEF family genes in Chenopodium quinoa

Jing Xiu-Qing1,2,3,*,Cai Yong-Duo1,Deng Ning1,Zhao Xiao-Dong1,2,Zhai Fei-Hong1,2,Zeng Qun4   

  1. 1 College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, Shanxi, China; 2 Shanxi Key Laboratory of Earth Surface Processes and Resource Ecology Security in Fenhe River Basin, Taiyuan Normal University, Jinzhong 030619, Shanxi, China; 3 College of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China; 4 School of Basic Medical, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi, China
  • Received:2025-07-22 Revised:2025-10-30 Accepted:2025-10-30 Published online:2025-11-10
  • Supported by:
    This study was supported by the Fundamental Research Programs of Shanxi Province (202203021212186), the Graduate Education and Innovation Projects of Taiyuan Normal University (SYYJSYC-2431), and the College Student Innovation and Entrepreneurship Training Program of Taiyuan Normal University (CXCY25104).

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摘要： ROP鸟嘌呤核苷酸交换因子(Rho-related GTPase of plants guanine nucleotide-exchange factors, RopGEFs)介导的ROP信号转导在植物细胞信号通路中起着关键作用。本研究利用生物信息学分析方法，从藜麦基因组中共鉴定到7个RopGEF家族基因(分布于6条染色体上)。基于系统进化关系和结构特征，将其与拟南芥、水稻等6种作物的共90个RopGEFs分为4个亚家族，其中CqRopGEF5与AtRopGEF1、OsRopGEF1亲缘关系较近。结构分析显示，同亚家族CqRopGEFs的外显子-内含子分布、蛋白Motifs组成及二级/3D结构均具保守性。实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction, qRT-PCR)结果表明，多数CqRopGEFs在种子萌发期高表达，且幼苗根中表达量高于茎、叶；其表达受外源ABA和非生物胁迫显著诱导，如ABA处理下CqRopGEF2/3/4/7表达先升后降，CqRopGEF7在冷、热胁迫后急剧下调。综上，CqRopGEFs家族结构进化保守，可能参与藜麦的生长发育、ABA信号通路及非生物胁迫响应。

关键词: 藜麦, RopGEFs, 系统进化分析, 基因表达, 非生物胁迫

Abstract:

In plants, RopGEF-mediated ROP signaling plays a crucial role in cellular signaling pathways. In this study, seven RopGEF family members were identified in Chenopodium quinoa through bioinformatics analysis and were found to be distributed across six chromosomes. Based on phylogenetic relationships and structural characteristics, 90 RopGEFs from six crop species, including Arabidopsis thaliana and rice, were classified into four subfamilies. Evolutionary analysis revealed that CqRopGEF5 is closely related to AtRopGEF1 and OsRopGEF1. Structural analysis indicated that the exon–intron organization, protein motif composition, and secondary and three-dimensional structures of CqRopGEFs are highly conserved. qRT-PCR analysis showed that most CqRopGEFs were highly expressed during seed germination, with expression levels in seedling roots being higher than in stems and leaves. Their expression was significantly induced by exogenous abscisic acid (ABA) and abiotic stresses. For instance, the expression of CqRopGEF2, CqRopGEF3, CqRopGEF4, and CqRopGEF7 initially increased and then decreased under ABA treatment, while CqRopGEF7 was strongly downregulated under both cold and heat stress. In conclusion, the CqRopGEF gene family exhibits conserved evolutionary structure and may play important roles in quinoa growth and development, ABA signaling, and responses to abiotic stress.

Key words: Chenopodium quinoa, RopGEFs, phylogenetic analysis, gene expression, abiotic stresses

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