一个新的水稻窄叶突变体nal24的基因定位及克隆

doi:10.3724/SP.J.1006.2026.62009

作物学报 ›› 2026, Vol. 52 ›› Issue (11): 3221-3231.doi: 10.3724/SP.J.1006.2026.62009

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

一个新的水稻窄叶突变体nal24的基因定位及克隆

赵汭彤^1,2，李毅林¹，周皖峰^1,2，方成¹，张东杨¹，尚丽君¹，程维民¹，陶亮之¹，吴跃进¹，武健东²，张从合³，叶亚峰^1,*，刘斌美^1,*

1 中国科学院合肥物质科学研究院, 安徽合肥 230031; 2 安徽农业大学, 安徽合肥 230036; 3 安徽荃银高科种业股份有限公司, 安徽合肥 230088

收稿日期:2026-03-22 修回日期:2026-08-21 接受日期:2026-08-21 出版日期:2026-11-12 网络出版日期:2026-09-01
通讯作者: 刘斌美, E-mail: liubm@ipp.ac.cn; 叶亚峰, E-mail: yyfeng@ipp.ac.cn
基金资助:
本研究由安徽省重点研究与开发计划项目(2023n06020006)，安徽省级科技创新攻坚计划项目(202523anull050002)，长三角科技创新共同体联合攻关(基础研究)项目(2024CSJZN01100)，国家自然科学基金青年项目C (32401738)和中国科学院青年促进会项目(2022454)资助。

Gene mapping and cloning of a novel narrow-leaf mutant nal24 in rice

Zhao Rui-Tong^1,2,Li Yi-Lin¹,Zhou Wan-Feng^1,2,Fang Cheng¹,Zhang Dong-Yang¹,Shang Li-Jun¹,Cheng Wei-Min¹,Tao Liang-Zhi¹,Wu Yue-Jin¹,Wu Jian-Dong²,Zhang Cong-He³,Ye Ya-Feng^1,*,Liu Bin-Mei^1,*

1 Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China; 2 Anhui Agricultural University, Hefei 230036, Anhui, China; 3 Winall Hi-tech Seed Co., Ltd., Hefei 230088, Anhui, China

Received:2026-03-22 Revised:2026-08-21 Accepted:2026-08-21 Published:2026-11-12 Published online:2026-09-01
Supported by:
This study was supported by the Key R&D Program of Anhui Province (2023n06020006), the Anhui Provincial Science and Technology Innovation Tackling Plan (202523anull050002), the Yangtze River Delta Science and Technology Innovation Community Joint Research (Basic Research) Project (2024CSJZN01100), the Youth Project C of the National Natural Science Foundation of China (32401738), and the Youth Promotion Association Program of the Chinese Academy of Sciences (2022454).

摘要/Abstract

摘要： 水稻叶片形态是影响光合效率与群体产量的关键农艺性状。本研究利用重离子束辐射诱变粳稻品种武运粳7号(wyj7)，获得一个稳定遗传的窄叶突变体nal24。与野生型相比，nal24突变体的叶片宽度极显著降低，且株高变矮、分蘖数增多。组织学分析表明，其窄叶表型源于大、小叶脉数量减少以及小叶脉间距缩小。光合特性测定显示，nal24突变体在不同光强下的净光合速率均显著低于野生型，表明其光合作用能力降低。遗传分析表明，该性状受单隐性核基因控制。利用图位克隆技术，将目的基因nal24精细定位于1号染色体上77.6 kb的物理区间内，测序发现该区间内编码磷脂酰丝氨酸合酶的基因LOC_Os01g02890发生了单碱基替换(T→A)，导致提前形成终止密码子。通过CRISPR/Cas9技术在野生型中敲除该基因，植株表现出窄叶表型，回补材料也恢复了野生型叶宽。表达分析显示，nal24突变体中多个NAL家族基因的表达显著下调，而叶片宽度负调控基因WL1显著上调。本研究克隆了调控水稻叶脉发育与叶片形态建成的新基因NAL24，为解析水稻叶形建成的分子机制提供了新的理论依据与基因资源。

关键词: 窄叶, NAL24, 基因定位, 水稻, 叶脉发育

Abstract: Rice leaf morphology is a key agronomic trait affecting photosynthetic efficiency and population yield. In this study, a stably inherited narrow-leaf mutant nal24 was obtained by heavy-ion beam irradiation mutagenesis of the japonica rice cultivar Wuyunjing 7 (wyj7). Compared with the wild type, the nal24 mutant exhibited a highly significant reduction in leaf width, accompanied by decreased plant height and increased tiller number. Histological analysis revealed that the narrow-leaf phenotype was caused by reduced numbers of large and small veins as well as reduced distances between small veins. Measurements of photosynthetic characteristics showed that the net photosynthetic rate of nal24 was markedly lower than that of the wild type under different light intensities, indicating reduced photosynthetic capacity. Genetic analysis demonstrated that this trait was controlled by a single recessive nuclear gene. The target gene NAL24 was finely mapped to a 77.6 kb physical interval on chromosome 1 via map-based cloning. Sequencing results identified a single base substitution (T→A) in LOC_Os01g02890, which encodes a phosphatidylserine synthase within this interval, resulting in a premature termination codon. Knockout of this gene in wild-type plants using the CRISPR/Cas9 system produced a narrow-leaf phenotype, and genetic complementation restored wild-type leaf width. Transcriptional analysis indicated that the expression levels of multiple NAL family genes were significantly downregulated in the nal24 mutant, whereas the negative regulator of leaf width, WL1, was significantly upregulated. In conclusion, this study cloned the novel gene NAL24 that regulates rice vein development and leaf morphogenesis. The identification of this gene provides a theoretical basis and genetic resources for elucidating the molecular mechanisms underlying rice leaf shape formation.

Key words: narrow leaf, NAL24, gene mapping, rice, leaf vein development

赵汭彤, 李毅林, 周皖峰, 方成, 张东杨, 尚丽君, 程维民, 陶亮之, 吴跃进, 武健东, 张从合, 叶亚峰, 刘斌美. 一个新的水稻窄叶突变体nal24的基因定位及克隆[J]. 作物学报, 2026, 52(11): 3221-3231.

Zhao Rui-Tong, Li Yi-Lin, Zhou Wan-Feng, Fang Cheng, Zhang Dong-Yang, Shang Li-Jun, Cheng Wei-Min, Tao Liang-Zhi, Wu Yue-Jin, Wu Jian-Dong, Zhang Cong-He, Ye Ya-Feng, Liu Bin-Mei. Gene mapping and cloning of a novel narrow-leaf mutant nal24 in rice[J]. Acta Agronomica Sinica, 2026, 52(11): 3221-3231.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2026.62009

https://zwxb.chinacrops.org/CN/Y2026/V52/I11/3221

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一个新的水稻窄叶突变体nal24的基因定位及克隆

Gene mapping and cloning of a novel narrow-leaf mutant nal24 in rice

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