花生TIFY基因家族分析及其在调控叶片形态建成中的潜在作用

doi:10.3724/SP.J.1006.2026.65020

作物学报 ›› 2026, Vol. 52 ›› Issue (11): 3248-3263.doi: 10.3724/SP.J.1006.2026.65020

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

花生TIFY基因家族分析及其在调控叶片形态建成中的潜在作用

彭振英^1,**,*，刘梦莹^2,**，李蕾蕾^1,3，孟静静¹，张佳蕾¹，李国卫¹，隋娜²，李新国^1,*，万书波^1,*

1 山东省大田作物生理生态与高效生产重点实验室(筹) / 山东省农业种质资源创新国际联合实验室 / 山东省农业科学院作物种质资源研究所, 山东济南 250100; 2 山东师范大学生命科学学院, 山东济南 250100; 3 沈阳农业大学生命科学学院, 辽宁沈阳 110866

收稿日期:2026-03-13 修回日期:2026-08-21 接受日期:2026-08-21 出版日期:2026-11-12 网络出版日期:2026-08-28
通讯作者: 彭振英, E-mail: pengzhenying2005@126.com; 李新国, E-mail: xinguol@163.com; 万书波, E-mail: wanshubo2016@163.com
基金资助:
本研究由国家重点研发计划项目(2024YFD2301001), 山东省自然科学基金项目(ZR2023MC109), 财政部和农业农村部国家现代农业产业技术体系建设专项(花生, CARS-13), 泰山学者工程和山东省农业科学院农业科技创新工程(CXGC2026H23)资助。

Comprehensive analysis of the TIFY gene family and its potential roles in regulating leaf morphogenesis in Arachis hypogaea

Peng Zhen-Ying^1,**,*,Liu Meng-Ying^2,**,Li Lei-Lei^1,3,Meng Jing-Jing¹,Zhang Jia-Lei¹,Li Guo-Wei¹,Sui Na²,Li Xin-Guo^1,*,Wan Shu-Bo^1,*

1 Shandong Provincial Key Laboratory of Field Crop Physiology, Ecology, and Efficient Production (Under Construction) / Shandong International Joint Laboratory of Agricultural Germplasm Resources Innovation / Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China; 2 College of Life Sciences, Shandong Normal University, Jinan 250100, Shandong, China; 3 College of Life Sciences, Shenyang Agricultural University, Shenyang 110866, Liaoning, China

Received:2026-03-13 Revised:2026-08-21 Accepted:2026-08-21 Published:2026-11-12 Published online:2026-08-28
Supported by:
This study was supported by the National Key Research and Development Program of China (2024YFD2301001), the Natural Science Foundation of Shandong Province (ZR2023MC109), the China Agriculture Research System of MOF and MARA (Peanut, CARS-13), the Taishan Scholars Project, and the Agricultural Science & Technology Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2026H23).

摘要/Abstract

摘要： TIFY基因家族在植物发育及对非生物和生物胁迫的响应中起着关键作用。本研究对花生中的TIFY成员进行了全基因组鉴定，结合功能分析和对卷叶突变体的验证，共鉴定到30个AhTIFY基因，分别属于TIFY、PPD、ZML和JAZ亚家族。其中，JAZ基因在各组织中表达水平较高，表明其在花生组织和器官发育中可能发挥更重要的作用。启动子分析揭示了若干与光、干旱、水杨酸和茉莉酸响应相关的顺式作用元件。蛋白互作分析表明，AhJAZ蛋白参与广泛的同源和异源多聚体互作，并与MYB和MYC等转录因子相互作用。比较正常植株与卷叶突变体的转录组，发现激素生物合成途径相关基因的表达模式存在显著差异。在本氏烟草中过表达AhJAZ8和AhJAZ20可诱导叶片卷曲，证明了AhTIFY基因在叶片形态建成中的作用。

关键词: 花生, TIFY基因家族, 全基因组鉴定, 卷叶, 叶片形态建成

Abstract: The TIFY gene family plays crucial roles in plant development and responses to abiotic and biotic stresses. Genome-wide identification of TIFY members in peanut was combined with functional analysis and validation using a peanut leaf-curling mutant. A total of 30 AhTIFY genes were identified and assigned to the TIFY, PPD, ZML, and JAZ subfamilies. Among these, JAZ genes showed higher expression levels across various tissues, suggesting a more significant role in peanut tissue and organ development. Promoter analysis revealed a number of cis-elements associated with responses to light, drought, salicylic acid, and jasmonic acid. Protein interaction analysis indicated that AhJAZ proteins engage in extensive homomeric and heteromeric interactions and interact with transcription factors such as MYB and MYC. Transcriptomic comparison between normal plants and the leaf-curling mutant revealed significant differences in the expression patterns of genes related to hormone biosynthesis. Overexpression of AhJAZ8 and AhJAZ20 in Nicotiana benthamiana induced leaf curling, demonstrating the role of AhTIFY genes in leaf morphogenesis.

Key words: Arachis hypogaea, TIFY gene family, genome-wide identification, leaf curling, leaf morphogenesis

彭振英, 刘梦莹, 李蕾蕾, 孟静静, 张佳蕾, 李国卫, 隋娜, 李新国, 万书波. 花生TIFY基因家族分析及其在调控叶片形态建成中的潜在作用[J]. 作物学报, 2026, 52(11): 3248-3263.

Peng Zhen-Ying, Liu Meng-Ying, Li Lei-Lei, Meng Jing-Jing, Zhang Jia-Lei, Li Guo-Wei, Sui Na, Li Xin-Guo, Wan Shu-Bo. Comprehensive analysis of the TIFY gene family and its potential roles in regulating leaf morphogenesis in Arachis hypogaea[J]. Acta Agronomica Sinica, 2026, 52(11): 3248-3263.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2026.65020

https://zwxb.chinacrops.org/CN/Y2026/V52/I11/3248

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花生TIFY基因家族分析及其在调控叶片形态建成中的潜在作用

Comprehensive analysis of the TIFY gene family and its potential roles in regulating leaf morphogenesis in Arachis hypogaea

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