一个FIF1基因的SNP及定位研究

作物学报 ›› 2007, Vol. 33 ›› Issue (02): 256-261.

一个FIF1基因的SNP及定位研究

韩志国;郭旺珍;张天真*

南京农业大学棉花研究所，作物遗传与种质创新国家重点实验室，江苏南京210095

收稿日期:2006-03-14 修回日期:1900-01-01 出版日期:2007-02-12 网络出版日期:2007-02-12
通讯作者: 张天真

Mapping of One Fiber Factor 1 Gene Based on Single Nucleotide Polymorphisms in Gossypium

HAN Zhi-Guo,GUO Wang-Zhen,ZHANG Tian-Zhen*

National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China

Received:2006-03-14 Revised:1900-01-01 Published:2007-02-12 Published online:2007-02-12
Contact: ZHANG Tian-Zhen

摘要/Abstract

摘要： 分子标记辅助选择逐步成为植物育种的重要方法。然而需要更多的实用的分子标记，尤其是与重要农艺性状紧密连锁的标记。目前，许许多多的方法已经应用到棉花结构基因组的标记开发，但是棉花SNP标记鲜见报道。FIF1基因是棉纤维优势表达的一个基因，研究证明它在棉纤维的发育过程中起着很重要的调控作用。根据已发表的亚洲棉FIF

关键词: SNAP, CAPs, 遗传图谱, FIF1, Gossypium

Abstract:

Marker-assisted selection (MAS) has been an important way for practical plant breeding, and more available markers are required, especially those linked to the genes of some agronomical important traits. Many kinds of molecular markers are being used in cotton genetic mapping, but there is little report on SNP markers development of cotton genes. FIF1 is predominantly expressed early in developing cotton fibers and identified to be a key regulator of cotton fiber development. In this paper, FIF1 genes from G. hirsutum cv. TM-1 and G. barbadense cv. Hai7124 were cloned on the basis of its published sequence in G. arboreum L. Based on the SNPs between FIF1 genes from two tetraploid cotton, SNP and CAPs markers were developed, and the gene was mapped on our allotetraploid cotton genetic map. This result indicates that SNP marker development is feasible and effective in Gossypium.

Key words: SNAP, CAPs, Genetic map, FIF1, Gossypium

韩志国;郭旺珍;张天真. 一个FIF1基因的SNP及定位研究[J]. 作物学报, 2007, 33(02): 256-261.

HAN Zhi-Guo;GUO Wang-Zhen;ZHANG Tian-Zhen. Mapping of One Fiber Factor 1 Gene Based on Single Nucleotide Polymorphisms in Gossypium[J]. Acta Agron Sin, 2007, 33(02): 256-261.

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