欢迎访问作物学报,今天是

作物学报 ›› 2008, Vol. 34 ›› Issue (10): 1781-1787.doi: 10.3724/SP.J.1006.2008.01781

• 耕作栽培·生理生化 • 上一篇    下一篇

高品质陆地棉蔗糖代谢关键酶活性对纤维品质形成的影响

刘娟;宋宪亮;朱玉庆;李学刚;陈二影;孙学振*   

  1. 山东农业大学农学院 / 作物生物学国家重点实验室, 山东泰安 271018
  • 收稿日期:2008-01-14 修回日期:1900-01-01 出版日期:2008-10-12 网络出版日期:2008-10-12
  • 通讯作者: 孙学振

Effects of Key Enzyme Activities in Sucrose Metabolism on Fiber Quality in High Quality Upland Cotton

LIU Juan,SONG Xian-Liang,ZHU Yu-Qing,LI Xue-Gang,CHEN Er-Ying,SUN Xue-Zhen*   

  1. Agronomy College, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, Shandong, China
  • Received:2008-01-14 Revised:1900-01-01 Published:2008-10-12 Published online:2008-10-12
  • Contact: SUN Xue-Zhen

摘要: 以高品质陆地棉鲁324系(324)、渝棉1号(YM-1)和普通陆地棉鲁棉研18 (L18)为材料, 研究蔗糖代谢关键酶活性变化对纤维品质形成的影响。结果表明, 高品质陆地棉叶片蔗糖磷酸合成酶(sucrose phosphate synthetase, SPS)活性高, 蔗糖合成能力强, 主茎叶和果枝叶蔗糖含量高, 蔗糖供应能力强。Logistic方程模拟纤维素累积过程, 高品质陆地棉纤维素进入快速累积期的时间晚, 终止期延后, 快速累积持续期长, 累积速率平缓, 最终纤维素含量高, 纤维比强度高。在纤维发育前期, 高品质陆地棉蔗糖贮存较多, 为次生壁发育提供了充足的初始底物。高品质陆地棉蔗糖合成酶(sucrose synthetase, SS)活性高, 降解蔗糖能力强, 酶活性迅速增加时期与纤维素快速累积期相吻合; SPS活性在纤维素快速累积期终止前均明显高于普通陆地棉。叶片SPS活性和棉纤维中SS、SPS活性与纤维素累积及纤维品质形成有密切关系。

关键词: 高品质陆地棉, 纤维品质, 蔗糖合成酶, 蔗糖磷酸合成酶

Abstract: Fiber strength is an important index that measures the fiber quality, which determined by cellulose deposition characteristics. Cellulose synthesis and accumulation are affected by many enzymes including sucrose synthetase (SS), sucrose phosphate synthetase (SPS) and so on. Up to date, there have been few reports about cellulose accumulation and key enzymes relating to sucrose metabolism in high quality upland cotton cultivars. To investigate the relationship between fiber quality differences and relating sucrose metabolism enzymes, a filed experiment was conducted at Shandong Agricultural University in 2006–2007. Three upland cotton cultivars including Ludimianlu 324 and Yumian1(YM-1) (high quality) and Lumianyan (18) (common quality) were used in the experiment. Leaves were sampled at 15, 30, 45, and 60 days after leaf unfolding. Flowers were labeled at anthesis and sampled every 7 days from 7 days after anthesis. The result showed that the sucrose content was higher with higher SPS activities in high quality upland cotton leaves. The simulation with Logistic equation of cotton cellulose accumulation showed that the accumulation duration was longer and the accumulation rate was moderate, so the final cellulose content was higher in high quality upland cotton cultivar. Compared with common upland cotton, the sucrose content was higher in fiber at early growth stage in high quality upland cotton, which supplied enough original substrate for secondary wall thickening. Meanwhile, the SS activity was higher and consistent with cellulose accumulation duration in high quality upland cotton. Furthermore, high quality upland cotton had higher SPS activity before termination of cellulose accumulation duration. It was suggested that SPS activity in leaves, SS and SPS activities in fibers had close correlation with cellulose accumulation and fiber quality.

Key words: High quality upland cotton (Gossypium hirsutum L.), Fiber quality, Sucrose synthetase, Sucrose phosphate synthetase

[1] 王晔, 刘钊, 肖爽, 李芳军, 吴霞, 王保民, 田晓莉. 转PSAG12-IPT基因对棉花叶片衰老及产量和纤维品质的影响[J]. 作物学报, 2021, 47(11): 2111-2120.
[2] 田景山, 张煦怡, 王文敏, 杨延龙, 随龙龙, 张鹏鹏, 张亚黎, 张旺锋, 勾玲. 棉花脱叶催熟剂对纤维品质的影响及应用时间的确定[J]. 作物学报, 2020, 46(9): 1388-1397.
[3] 李超,李志坤,谷淇深,杨君,柯会锋,吴立强,王国宁,张艳,吴金华,张桂寅,阎媛媛,马峙英,王省芬. 海岛棉CSSLs分子评价及纤维品质、产量性状QTL定位[J]. 作物学报, 2018, 44(8): 1114-1126.
[4] 沈超,李定国,聂以春,林忠旭. 利用黄褐棉染色体片段导入系定位产量和纤维品质性状QTL[J]. 作物学报, 2017, 43(12): 1733-1745.
[5] 许乃银,李健. 利用GGE双标图划分长江流域棉花纤维品质生态区[J]. 作物学报, 2014, 40(05): 891-898.
[6] 李彦生,南海洋,杜明,连腾祥,张秋英,刘晓冰. 菜用大豆籽粒不同部位蔗糖积累及关键酶活性研究[J]. 作物学报, 2013, 39(11): 2099-2105.
[7] 陈维维,再吐尼古丽?库尔班,涂振东,叶凯. 不同种植密度对甜高粱糖分积累及SS、SPS活性的影响[J]. 作物学报, 2013, 39(08): 1507-1513.
[8] 李学刚, 宋宪亮, 孙学振, 陈二影, 张美玲, 赵庆龙. 控释氮肥对棉花纤维品质、产量与氮肥利用效率的影响[J]. 作物学报, 2011, 37(10): 1910-1915.
[9] 张祥, 刘晓飞, 吕春花, 董召娣, 陈源, 陈德华. 两个不同类型彩色棉品种纤维发育的生理特征[J]. 作物学报, 2011, 37(03): 489-495.
[10] 陈源,王永慧,肖健,栾娜,张祥,陈德华. 高品质陆地棉棉铃发育特点[J]. 作物学报, 2010, 36(08): 1371-1376.
[11] 张美玲,宋宪亮,孙学振,陈二影,赵庆龙,李宗泰. 彩色棉纤维发育过程中超分子结构变化与纤维品质的关系[J]. 作物学报, 2010, 36(08): 1386-1392.
[12] 孔广超,秦利,徐海明,祝水金. 棉花IF2群体构建及其在纤维品质遗传和杂种优势研究中的应用[J]. 作物学报, 2010, 36(06): 940-944.
[13] 琚铭, 王海棠, 王立科, 李飞飞, 吴慎杰, 朱华玉, 张天真, 郭旺珍. 棉纤维发育相关基因时空表达与纤维品质的关联分析[J]. 作物学报, 2009, 35(7): 1217-1228.
[14] 张祥,肖建,栾娜,王永慧,杨朝华,陈源,陈德华*. 两个不同彩色棉纤维品质性状形成特点及生长调节剂调节作用[J]. 作物学报, 2009, 35(5): 907-913.
[15] 杨鑫雷,王志伟,张桂寅,潘玉欣,吴立强,李志坤,王省芬,马峙英. 棉花分子遗传图谱构建和纤维品质性状QTL分析[J]. 作物学报, 2009, 35(12): 2159-2166.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!