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作物学报 ›› 2008, Vol. 34 ›› Issue (12): 2059-2069.doi: 10.3724/SP.J.1006.2008.02059

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

大豆育成品种农艺性状QTL与SSR标记的关联分析

张军1,2;赵团结1;盖钧镒1,*   

  1. 1南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏南京210095；2滨州职业学院，山东滨州256603
  • 收稿日期:2008-04-08 修回日期:2008-07-13 出版日期:2008-12-12 网络出版日期:2008-10-10
  • 通讯作者: 盖钧镒
  • 基金资助:

    国家重点基础研究发展规划(973规划)项目(2006CB101708);国家高技术研究发展计划(863计划)项目(2006AA100104);国家自然科学基金项目(30490250,30671266);教育部高等学校创新引智计划(B08025);农业部公益性行业专项(200803060)

Association Analysis of Agronomic Trait QTLs with SSR Markers in Released Soybean Cultivars

ZHANG Jun12,ZHAO Tuan-Jie1,GAI Jun-Yi1*   

  1. 1Soybean Research Institute, National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu; 2Binzhou Vocational College, Binzhou 256603, Shandong, China
  • Received:2008-04-08 Revised:2008-07-13 Published:2008-12-12 Published online:2008-10-10
  • Contact: GAI Jun-Yi

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2962

被引次数

166

摘要：

利用85个SSR标记，对大豆育成品种群体(190份代表性材料)的基因组进行扫描，在检测群体结构基础上搜索连锁不平衡位点，并采用TASSEL软件的GLM方法对11个大豆农艺性状QTL进行关联分析。结果表明：(1) 在公共图谱上共线性或非共线性的SSR位点组合均广泛存在连锁不平衡(LD)，但不平衡程度D′>0.5的组合数只占总位点组合的1.71%，共线位点D′值随遗传距离衰减较快；(2) SSR数据遗传结构分析表明，育成品种群体由7个亚群体组成，矫正后全群体共有45个位点累计136个位点(次)与11个大豆农艺性状QTL关联，其中22个位点(次)与家系连锁定位的QTL区间相重，有43个位点(次) 2年重复出现；(3) 一些标记同时与2个或多个性状关联，可能是性状相关或一因多效的遗传基础；(4) 育成品种群体关联位点与地方品种群体和野生群体只有少数相同，群体间育种性状的遗传结构有相当大差异；(5) 发掘出农艺性状优异等位变异及其载体品种，包括增效最大的产量等位变异Satt347-300 (+932 kg hm-2，中豆26)，生物量等位变异Satt365-294(+3 123 kg hm-2，黄毛豆)，蛋白质含量等位变异Be475343-198 (+0.41%，淮豆4号)，脂肪含量等位变异Satt150-273 (+2.32%，科丰15)等。在此基础上作了设计育种的探讨。

关键词: 大豆育成品种, 农艺性状, SSR标记, 关联分析, 优良等位变异, 设计育种

Abstract:

The genotyping data of 85 simple-sequence repeat (SSR) markers on released cultivar population (RCP) (a representative sample with 190 accessions) were obtained and analyzed for LD of pairwise loci and population structure, and then for association between SSR loci and 11 soybean agronomic traits under TASSEL GLM (General linear model) program. The results showed that: (1) LD was detected extensively not only among syntenic markers but also among nonsyntenic ones, while the loci pairs with D′>0.5 accounted for only 1.71% of the total ones. The syntenic D′ value attenuated fastly along with the increase of genetic distance. (2) Genetic structure analysis showed that RCP was composed of seven subpopulations. The 45 SSR loci with a total of 136 loci (time) were found to be associated with 11 agronomic traits in the RCP. Among those, 22 loci (times) were consistent with mapped QTLs from family-based linkage mapping procedure and 43 loci (times) were consistently detected in two experiment years. (3) There also found a certain number of loci associated simultaneously with two or more traits, which might be the genetic reason of correlation among traits as well as the pleiotropic effects of gene(s). (4) There found only a few association loci in released cultivar population coincided with those in landrace and wild populations, indicating the large difference of genetic structure between the populations. (5) The elite alleles of the agronomic traits along with their carriers were detected, such as the allele Satt347-300 for largest positive yield effect (+932 kg hm-2 for Zhongdou 26)，Satt365-294 for biomass(+3 123 kg hm-2 for Huangmaodou)，Be475343-198 for protein content (+0.41% for Huaidou 4)，Satt150-273 for oil content (+2.32% for Kefeng 15), and were used to illustrate their potential in breeding programs.

Key words: Released soybean cultivar, Agronomic trait, SSR marker, Association analysis, Elite allele, Breeding by design

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