作物学报 ›› 2010, Vol. 36 ›› Issue (05): 788-793.doi: 10.3724/SP.J.1006.2010.00788
马雄风1,3,喻春明1,唐守伟1,郭三堆2,张锐2,王延周1,朱爱国1,朱四元1,熊和平1,*
MA Xiong-Feng1,3,YU Chun-Meng1,TANG Shou-Wei1,GUO San-Dui2,ZHANG Dui2,WANG Yan-Zhou1,SHU Ai-Guo1,SHU Si-Yuan1,XIONG He-Beng1*
摘要:
利用苎麻下胚轴高频再生体系,将携带人工合成的CryIA杀虫基因和CpTI基因的高效双价杀虫基因的植物表达载体pGBI4ABC转化到苎麻主栽品种中苎1号中。经根癌农杆菌侵染和共培养后,用50 mg L–1卡那霉素+ 300 mg L–1头孢霉素筛选共获得32株抗性植株; PCR检测显示26株为阳性, 占80%。Southern杂交结果证实, 外源基因已经整合到苎麻的基因组中。这项研究为最终创造兼抗鳞翅目及鞘翅目等害虫的苎麻种质材料奠定了基础。
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