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作物学报 ›› 2011, Vol. 37 ›› Issue (01): 165-169.doi: 10.3724/SP.J.1006.2011.00165

• 研究简报 • 上一篇    下一篇

红麻五个质量性状在遗传连锁图谱中的初步定位

陈美霞,祁建民*,危成林,谢增荣,林培清,兰涛,陶爱芬,陈涛   

  1. 福建农林大学 / 作物遗传育种与综合利用省部共建教育部重点实验室，福建福州 350002
  • 收稿日期:2010-07-06 修回日期:2010-09-29 出版日期:2011-01-12 网络出版日期:2010-11-16
  • 通讯作者: 祁建民, E-mail: Qijm863@163.com, Tel: 0591-87644898
  • 基金资助:

    本研究由国家自然科学基金项目(30571188)和国家麻类产业技术体系建设(nycytx-19-E05)项目资助。

Preliminary Localization of Five Qualitative Traits in Kenaf Genetic Linkage Map

CHEN Mei-Xia,QI Jian-Min*,WEI Cheng-Lin,XIE Zeng-Rong,LIN Pei-Qing,LAN Tao,TAO Ai-Fen,CHEN Tao   

  1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops / Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2010-07-06 Revised:2010-09-29 Published:2011-01-12 Published online:2010-11-16
  • Contact: QI Jian-Min,E-mail:Qijm863@163.com,Tel:0591-87644898

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被引次数

4

摘要： 红麻遗传连锁图谱的构建和性状的基因定位，可促进红麻的分子辅助育种研究的发展。本研究以埃及的阿联红麻与福建农林大学育成的高产优质抗病新品种福红992杂交，F1和F2自交衍生162个F2:3家系为材料，在笔者已完成的红麻遗传连锁图谱构建的工作基础上，对红麻的叶柄色、叶形、花冠大小、花冠形状、后期茎色5个质量性状进行基因定位。用Mapmaker/Exp 3.0软件进行连锁分析，结果表明，后期茎色基因与叶柄色基因连锁，存在紧密的连锁关系，其遗传距离为2.8 cM，定位于第5条连锁群；花冠大小与花冠形状这2个基因之间也存在一定的连锁关系，其遗传距离为14.7 cM，定位于第6条连锁群，叶型与花冠大小和花冠形状的遗传距离分别为38.2 cM 与23.5 cM，虽然都定位于第6条连锁群，但是否存在连锁关系有待进一步研究。所获结果在红麻遗传学和育种学上有一定的现实意义和分子辅助育种实用价值。

关键词: 红麻, 质量性状, 遗传连锁图谱定位

Abstract: With the genetic linkage map construction for kenaf and quantitative trait loci mapping, it is possible to perform marker-assisted selection to improve kenaf agronomical important traits at molecular level. In this investigation, using a population with 162 F2:3 individuals from the cross between Alian kenaf (from Egypt) and Fuhong 992 (from Fujian agriculture and forestry university) with high quality and disease resistance, on the basis of the linkage map we had finished, we finally mapped five qualitative trait genes for leaf petiole color, leaf shape, dimension of corolla, corolla shape, stem color at late stage. Analysis using the Mapmaker/Exp 3.0 software showed that there were close linkage relation between the gene of stem color at late stage and leaf petiole color gene, with a genetic distance of 2.8 cM, which were mapped on the linkage group 5. Also linkage existed between dimension of corolla and corolla shape, with a genetic distance of 14.7 cM, which were mapped on the linkage group 6. The genetic distance between leaf shape and dimension of corolla, corolla shape, were 38.2 cM and 23.5 cM, respectively. They were mapped on the linkage group 6, but their linkage relation still requires further research. The results had certain practical significance and by molecular marker-assisted selection were useful for the research of kenaf genetics and the breeding.

Key words: Kenaf, Quality traits, Genetic linkage map location

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