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作物学报 ›› 2017, Vol. 43 ›› Issue (08): 1170-1180.doi: 10.3724/SP.J.1006.2017.01170

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

红麻EST-SSR标记的开发及其多态性评价

万雪贝1,2,李东旭1,2,徐益1,2,徐建堂1,张力岚1,2,张列梅1,林荔辉1,祁建民1,*,张立武1,2,*   

  1. 1福建农林大学作物遗传育种与综合利用教育部重点实验室 / 福建省作物设计育种重点实验室 / 作物科学学院; 2福建农林大学海峡联合研究院基因组与生物技术中心, 福建福州 350002
  • 收稿日期:2016-07-04 修回日期:2017-04-20 出版日期:2017-08-12 网络出版日期:2017-05-08
  • 通讯作者: 祁建民, E-mail: qijm863@163.com, Tel: 0591-87644898; 张立武, E-mail: lwzhang@fafu.edu.cn / zhang_liwu@hotmail.com
  • 基金资助:

    本研究由福建农林大学杰出青年科研基金项目(xjq201401), 国家现代农业产业技术体系建设专项(CARS-19-E06), 农业部东南黄红麻实验观测站(农科教发2011), 中贝粮油及特色经济作物引育种及新品种示范推广项目(2015I0001)和福建省麻类种质资源共享平台项目(2010N2002)资助。

Development and Polymorphism Evaluation of EST-SSR Markers in Kenaf

WAN Xue-Bei1,2,LI Dong-Xu1,2,XU Yi1,2,XUJian-Tang1,ZHANG Li-Lan1,2,ZHANGLie-Mei1,LINLi-Hui1,QI Jian-Min1,*,ZHANG Li-Wu1,2,*   

  1. 1 Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops of Ministry of Education / Fujian Key Laboratory for Crop Breeding by Design / College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2016-07-04 Revised:2017-04-20 Published:2017-08-12 Published online:2017-05-08
  • Contact: 祁建民, E-mail: qijm863@163.com, Tel: 0591-87644898; 张立武, E-mail: lwzhang@fafu.edu.cn / zhang_liwu@hotmail.com
  • Supported by:

    This study was supported by the Distinguished Young Research Fund in Fujian Agriculture and Forestry University (xjq201401), the China Agriculture Research System(CARS-19-E06),the Experiment Station of Jute and Kenaf in Southeast China (Nongkejiaofa 2011), the Introduction Breeding and Varieties Demonstration of Featured Crops between China and Benin (2015I0001), and Construction of Germplasm Resources Platform for Bast Fiber Crops in Fujian, China (2010N2002).

摘要:

从红麻90175个EST序列中挑出含有转录因子的EST,开发了94对SSR引物。以24份不同红麻种质资源的DNA为模板,利用9%非变性聚丙烯酰胺凝胶电泳检测多态性。结果表明,85(90.4%)对引物至少在2个材料之间存在多态性,表明开发的EST-SSR具有很好的多态性。其中,三核苷酸重复所占比例最多,重复基元(AAT)n和(ATG)n的多态性较高遗传多样性分析表明,24份红麻种质资源的遗传相似系数变化在0.62~0.92之间,表现出丰富的遗传基础。这些结果不仅丰富了红麻的分子标记数量,而且有助于红麻遗传育种研究。

关键词: 红麻, SSR, 遗传多样性, 标记开发

Abstract:

 In this study, 94 SSR primers were developed on the basis of the sequences containing transcription factors from 90175 expressed sequence tags (ESTs) in kenaf. A total of 24 kenaf accessions were used to evaluate the polymorphism of these developed SSRs using 9% polyacrylamide gel electrophoresis. Eighty-five SSR primers (90.4%) were successfully amplified and (ATG)n were the dominant motif. Genetic diversity analysis showed that the genetic similarity coefficient varied from 0.62 to 0.92, indicating an abundant genetic basis among these accessions. These developed EST-SSRs not only enrich molecular markers, but also facilitate genetics and breeding research in kenaf.

Key words: Kenaf (Hibiscus cannabinus), Simple sequence repeat, Genetic diversity, Marker development

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