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作物学报 ›› 2017, Vol. 43 ›› Issue (08): 1170-1180.doi: 10.3724/SP.J.1006.2017.01170

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

红麻EST-SSR标记的开发及其多态性评价

万雪贝1,2,李东旭1,2,徐益1,2,徐建堂1,张力岚1,2,张列梅1,林荔辉1,祁建民1,*,张立武1,2,*   

  1. 1福建农林大学作物遗传育种与综合利用教育部重点实验室 / 福建省作物设计育种重点实验室 / 作物科学学院; 2福建农林大学海峡联合研究院基因组与生物技术中心, 福建福州 350002
  • 收稿日期:2016-07-04 修回日期:2017-04-20 出版日期:2017-08-12 网络出版日期:2017-05-08
  • 通讯作者: 祁建民, E-mail: qijm863@163.com, Tel: 0591-87644898; 张立武, E-mail: lwzhang@fafu.edu.cn / zhang_liwu@hotmail.com
  • 基金资助:

    本研究由福建农林大学杰出青年科研基金项目(xjq201401), 国家现代农业产业技术体系建设专项(CARS-19-E06), 农业部东南黄红麻实验观测站(农科教发2011), 中贝粮油及特色经济作物引育种及新品种示范推广项目(2015I0001)和福建省麻类种质资源共享平台项目(2010N2002)资助。

Development and Polymorphism Evaluation of EST-SSR Markers in Kenaf

WAN Xue-Bei1,2,LI Dong-Xu1,2,XU Yi1,2,XUJian-Tang1,ZHANG Li-Lan1,2,ZHANGLie-Mei1,LINLi-Hui1,QI Jian-Min1,*,ZHANG Li-Wu1,2,*   

  1. 1 Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops of Ministry of Education / Fujian Key Laboratory for Crop Breeding by Design / College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2016-07-04 Revised:2017-04-20 Published:2017-08-12 Published online:2017-05-08
  • Contact: 祁建民, E-mail: qijm863@163.com, Tel: 0591-87644898; 张立武, E-mail: lwzhang@fafu.edu.cn / zhang_liwu@hotmail.com
  • Supported by:

    This study was supported by the Distinguished Young Research Fund in Fujian Agriculture and Forestry University (xjq201401), the China Agriculture Research System(CARS-19-E06),the Experiment Station of Jute and Kenaf in Southeast China (Nongkejiaofa 2011), the Introduction Breeding and Varieties Demonstration of Featured Crops between China and Benin (2015I0001), and Construction of Germplasm Resources Platform for Bast Fiber Crops in Fujian, China (2010N2002).

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摘要：

从红麻90175个EST序列中挑出含有转录因子的EST，开发了94对SSR引物。以24份不同红麻种质资源的DNA为模板，利用9%非变性聚丙烯酰胺凝胶电泳检测多态性。结果表明，85(90.4%)对引物至少在2个材料之间存在多态性，表明开发的EST-SSR具有很好的多态性。其中，三核苷酸重复所占比例最多，重复基元(AAT)n和(ATG)n的多态性较高。遗传多样性分析表明，24份红麻种质资源的遗传相似系数变化在0.62~0.92之间，表现出丰富的遗传基础。这些结果不仅丰富了红麻的分子标记数量，而且有助于红麻遗传育种研究。

关键词: 红麻, SSR, 遗传多样性, 标记开发

Abstract:

 In this study, 94 SSR primers were developed on the basis of the sequences containing transcription factors from 90175 expressed sequence tags (ESTs) in kenaf. A total of 24 kenaf accessions were used to evaluate the polymorphism of these developed SSRs using 9% polyacrylamide gel electrophoresis. Eighty-five SSR primers (90.4%) were successfully amplified and (ATG)n were the dominant motif. Genetic diversity analysis showed that the genetic similarity coefficient varied from 0.62 to 0.92, indicating an abundant genetic basis among these accessions. These developed EST-SSRs not only enrich molecular markers, but also facilitate genetics and breeding research in kenaf.

Key words: Kenaf (Hibiscus cannabinus), Simple sequence repeat, Genetic diversity, Marker development

[1]熊和平. 麻类作物育种学. 北京: 中国农业科学技术出版社, 2008. pp 156–185
Xiong H P. Breeding science of bast fiber Crops. Beijing: China Agricultural Science and Technology Press, 2008. pp 156–185 (in Chinese)
[2]陶爱芬, 祁建民, 林培青, 方平平, 吴建梅, 林荔辉. 红麻优异种质产量和品质性状主成分聚类分析与综合评价. 中国农业科学, 2008, 41: 2859–2867
Tao A F, Qi J M, Lin P Q, Fang P P, Wu J M, Lin L H. Cluster analysis and evaluation of elite kenaf germplasm based on principal components. SciAgricSin, 2008, 41: 2859–2867 (in Chinese with English abstract)
[3]祁建民, 陈幼玉, 周瑞阳, 林荔辉, 梁康迳, 吴建梅, 方平平. 红麻产量和纤维品质性状的遗传效应与杂种优势分析. 作物学报, 2005, 31: 469–475
Qi J M, Chen Y Y, Zhou R Y, Lin L H , Liang K J, Wu J M, Fang P P. Genetic effects and heterosis analysis for yield and quality traits in kenaf (Hibiscus cannabinusL.). ActaAgron Sin, 2005, 31: 469–475 (in Chinese with English abstract)
[4]颜婷婷. 黄、红麻纤维的木质素结构特征及其脱胶研究. 东华大学博士学位论文, 上海, 2011
Yan T T. Research on lignin structural characterization and degumming process of jute and kenaf fiber. PhD Dissertation Donghua University, Shanghai, China, 2011 (in Chinese with English abstract)
[5]李志明, 吴星娥, 周景辉. 红麻全秆纸地膜保温与降解应用试验. 中国造纸, 2004, (8): 22–24
Li Z M, Wu X, Zhou J H, Kenaf Mulch: Heat Preservation and Biodegradation. China Pulp &Paper, 2004, (8): 22–24 (in Chinese with English abstract)
[6]袁小红, 陈东生, 倪海燕. 黄/红麻在纺织领域的应用探讨. 中国麻业科学, 2007, (4): 217–220
Yuan X H, Chen D S, Ni H Y. Discussion on the application of jute and kenaf in textile field. Plant Fiber Sci China, 2007, (4): 217–220 (in Chinese with English abstract)
[7]Zhang L, Li A, Wang X, Xu J, Zhang G, Su J, Qi J, Guan C. Genetic diversity of kenaf (Hibiscus cannabinus) evaluated by inter-simple sequence repeat (ISSR). Biochem Genet, 2013, 51: 800–810
[8]Cheng X, Xu J, Xia S, Gu J, Yang Y, Fu J, Qian X, Zhang S, Wu J, Liu K. Development and genetic mapping of microsatellite markers from Genome survey sequences in Brassica napus. TheorAppl Genet, 2009, 118: 1121–1131
[9]Zhang L, Cai R, Yuan R, Tao A, Xu J, Lin L, Fang P, Qi J. Genetic diversity and DNA fingerprinting in jute (Corchorus spp.) based on SSR markers. The Crop J, 2015, 3: 416-422.
[10]Liu T, Zhu S, Fu L, Tang Q, Yu Y, Chen P, Luan M, Wang C, Tang S. Development and characterization of 1827 expressed sequence tag-derived simple sequence repeat markers for ramie (Boehmerianivea L. Gaud). PLoS One, 2013, 8: e60346
[11]Zhang L, Li Y, Tao A, Fang P, Qi J. Development and characterization of 1906 EST-SSR markers from unigenes in jute (Corchorus spp.). PLoS One, 2015, 10: e0140861
[12]潘海涛, 汪俊君, 王盈盈, 齐照良, 李斯深. 小麦EST-SSR标记的开发和遗传作图. 中国农业科学, 2010, 43: 452–461
Pan H T, Wang J J, Wang Y Y, Qi Z L, Li S S. Development and mapping of EST-SSR markers in wheat. SciAgric Sin, 2010, 43: 452–461 (in Chinese with English abstract)
[13]常玮, 赵雪, 李侠, 邱波, 韩英鹏, 縢伟丽, 李文滨. 大豆EST-SSR标记开发及与Genomic-SSR的比较研究. 中国油料作物学报, 2009, 31: 149–156
Chang W, Zhao X, Li X, Qiu B, Han Y P, Teng W L, Li W B. Development of soybean EST-SSR marker and comparison with Genomic-SSR marker. Chin J Oil Crop Sci, 2009, 31: 149–156 (in Chinese with English abstract)
[14]王庆彪, 张扬勇, 庄木, 杨丽梅, 刘玉梅, 吕红豪, 方智远. 中国50个甘蓝代表品种EST-SSR指纹图谱的构建. 中国农业科学, 2014, 47: 111–121
Wang Q B, Zhang Y Y, Zhuang M, Yang L M, Liu Y M, Lyu H H, Fang Z H. EST-SSR fingerprinting of fifty cabbage representative varieties from China. SciAgric Sin, 2014, 47: 111–121 (in Chinese with English abstract)
[15]林珲, 朱海生, 陈敏氡,王彬, 温庆放, 吴卫东. 花椰菜EST-SSR反应体系的优化. 分子植物育种, 2015, 13: 2610–2615
Lin H, Zhu H S, Chen M D, Wang B, Wen Q F, Wu W D. Establishment and optimization of EST-SSR PCR amplification system in cauliflower. MolPlant Breed, 2015, 13: 2610–2615
[16]刘晨晨, 栾明宝, 陈建华, 王晓飞, 许英, 孙志民. 苎麻Genomic-SSR与EST-SSR分子标记遗传差异性分析. 中国麻业科学, 2015, (2): 57–63
Liu C C, Luan M B, Chen J H, Wang X F, Xu Y, Sun Z M. Genetic differences in ramie analyzed between genomic-SSR and EST-SSR. Plant FiberSciChina, 2015, (2): 57–63
[17]武耀龙. 红麻遗传连锁图谱构建及重要数量性状QTL分析. 中国农业科学院硕士学位论文, 北京, 2014
Wu Y L. Construction Linkage Map and QTL Analysis of Essential Quantitatives Traits for kenaf (Hibiscus cannabinusL.). MS Thesis of Chinese Academy of Agricultural Science, Beijing, China, 2014 (in Chinese with English abstract)
[18]徐伟. 不同基因型红麻及其近缘种亲缘关系的RAPD分析. 安徽农业大学硕士学位论文, 安徽合肥, 2005
Xu W. Relationships of Various Genotypes of Hibiscus cannabinusL. and Its Related Species Based on RAPD Markers Analysis. MS Thesis of Anhui Agricultural University, Hefei, China, 2005 (in Chinese with English abstract)
[19]Zhang L, Wan X, Xu J, Lin L, Qi J. De novo assembly of kenaf (Hibiscus cannabinus) transcriptome using Illumina sequencing for gene discovery and marker identification. Mol Breed, 2015, 10: 192–202
[20]Rohlf F. NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.10. Exeter Software, New York, 2002
[21]Bostsein D, White R L, Skolnick M. Construction of a genetic linkage map in man using restriction fragment polymorphisms. Am J Hum Genet, 1980, 32: 314–331
[22]郑海燕, 粟建光, 戴志刚, 李燕, 陈基权, 龚友才. 红麻种质资源SRAP指纹图谱构建及遗传多样性分析. 湖北农业科学, 2011, 50: 411–415
Zhen H Y, Su J G, Dai Z G, Li Y, Chen J Q, Gong Y C. Fingerprint construction and genetic diversity analysis of kenaf germplasm by SRAP markers. Hubei AgricSci, 2011, 50: 411–415 (in Chinese with English abstract)
[23]Hong C, Piao Z, Kang T, Batley J, Yang T, Hur Y, Bhak J, Park B, Edwards D, Lim Y. Genomic distribution of simple sequence repeats in Brassica rapa. Mol Cells, 2007, 23: 349–356
[24]Goff S, Ricke D, Lan T, Presting G, Wang R, Dunn M, Glazebrook J, Sessions A, Oeller P, Varma H, Hadley D, Hutchison D, Martin C, Katagiri F, Lange B, Moughamer T, Xia Y, Budworth P, Zhong J, Miguel T, Paszkowski U, Zhang S, Colbert M, Sun W, Chen L, Cooper B, Park S, Wood T, Mao L, Quail P, Wing R, Dean R, Yu Y, Zharkikh A, Shen R, Sahasrabudhe S, Thomas A, Cannings R, Gutin A, Pruss D, Reid J, Tavtigian S, Mitchell J, Eldredge G, Scholl T, Miller R M, Bhatnagar S, Adey N, Ruba T, Tusneem N, Robinson R, Feldhaus J, Macalma T, Oliphant A, Briggs S. A draft sequence of the rice Genome (Oryza sativa L. ssp. japonica). Science, 2002, 296: 92–100
[25]刘强, 张贵友, 陈受宜. 植物转录因子的结构与调控作用. 科学通报, 2000, 45: 1465–1474
Liu Q, Zhang G Y, Chen S Y. The structure and regulation of plant transcription factors. Chin Sci Bull, 2000, 45: 1465–1474 (in Chinese with English abstract)
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