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作物学报 ›› 2011, Vol. 37 ›› Issue (03): 415-423.doi: 10.3724/SP.J.1006.2011.00415

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

花生AhFAD2基因的多态性及其与籽粒油酸/亚油酸比值间的相关性

周丽侠1,3,唐桂英1,2,陈高1,3,毕玉平1,2,3,单雷1,2,*   

  1. 1 山东省农业科学院高新技术研究中心 / 山东省作物与畜禽品种改良生物技术重点实验室，山东济南 250100；2 农业部黄淮海作物遗传改良与生物技术重点实验室，山东济南 250100; 3 山东师范大学生命科学学院，山东济南 250014
  • 收稿日期:2010-08-02 修回日期:2010-12-03 出版日期:2011-03-12 网络出版日期:2011-01-17
  • 基金资助:

    本研究由国家自然科学基金项目(30971546)和山东省自然科学基金项目(ZR2009DM013)资助。

Correlation between AhFAD2 Polymorphism and Oleic Acid/ Linoleic Acid (O/L) Ratio in Peanut Seeds

ZHOU Li-Xia1,3,TANG Gui-Ying1,2,CHEN Gao1,3,BI Yu-Ping1,2,3,SHAN Lei1,2,*   

  1. 1 Hi-Tech Research Centre, Shandong Academy of Agricultural Sciences and Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province, Jinan 250100, China; 2 Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China; 3 College of Life Science, Shandong Normal University, Jinan 250014, China
  • Received:2010-08-02 Revised:2010-12-03 Published:2011-03-12 Published online:2011-01-17

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1357

被引次数

7

摘要： 高油酸低亚油酸的花生油稳定性好、营养价值高，培育高油酸/亚油酸比值(O/L)的花生品种一直是花生育种的重要目标。为深入了解控制花生O/L比值的遗传基础，提高花生品质育种效率，对鲁花14等13个花生品种Δ12脂肪酸脱氢酶AhFAD2基因的编码区进行了序列分析。结果表明，该基因在13个花生品种中皆存在3类转录本，即AhFAD2A、AhFAD2B和假基因。台山珍珠、台山三粒肉、江田种、Chico、05-21063、白沙1016的基因型是OL1OL1OL2OL2；临桂麻壳、飞龙乡、勾了种、鲁花14、花育19、花育23的基因型是ol1ol1OL2OL2；鲁花11的基因型可能存在OL1ol1杂合等位位点。在个别品种中AhFAD2A基因的若干位置存在多态性；AhFAD2B基因相对保守，所测13个花生品种的核苷酸序列基本相同。结合13个花生栽培品种籽粒中O/L比值测定的结果，初步探讨了基因多态性与O/L比值之间的关系，同时对假基因可能存在的功能进行了讨论。

关键词: 花生, Δ12脂肪酸脱氢酶基因, 基因多态性, O/L比值, 假基因

Abstract: Peanut oils containing high levels of oleic acid and low linoleic acid levels are strongly demanded. This would improve oil stability and nutritional quality. Thus, breeding peanut variety with high O/L ratio has become one of the major goals in plant breeding. To investigate the genetic traits that control high oleate levels-phenotype and improve the peanut-breeding efficiency, we sequenced ORF region of about 527 delta 12 fatty acid desaturaseAhFAD2 cDNAs from 13 peanut varieties. Our results showed that three types of mRNA transcripts AhFAD2A, AhFAD2B and pseudogene existed in all peanut varieties. Genotypes of peanut varieties TaishanPearl, Taishan Sanlirou, Jiangtian, Chico, 05-21063, Baisha 1016; and Linguimake, Feilongxiang, Goule, Luhua 14, Huayu 19, Huayu 23, and Luhua 11 were found to be OL1OL1OL2OL2, ol1ol1OL2OL2 and OL1ol1OL2OL2, respectively. We also found thatsome SNP polymorphism sites existed in the AhFAD2A gene but not in all peanut varieties, and AhFAD2B was relatively conserved in all 13 peanut varieties investigated. Combined with the measurement of O/L ratio in various peanut varieties, the relationship between gene polymorphism and O/L ratio was primarily investigated. Meanwhile, the potential function of pseudogene was also discussed.

Key words: Arachis hypogaea L., Δ12 fatty acid desaturase gene, Gene polymorphism, O/L ratio, Pseudogene

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