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作物学报 ›› 2012, Vol. 38 ›› Issue (04): 754-759.doi: 10.3724/SP.J.1006.2012.00754

• 研究简报 • 上一篇    

花生的荧光显带和rDNA荧光原位杂交核型分析

佘朝文1,2,3，张礼华1，蒋向辉1,2,3   

  1. 1 怀化学院生命科学系，湖南怀化 418008；2 怀化学院民族药用植物资源研究与利用湖南省重点实验室，湖南怀化 418008；3 怀化学院湘西药用植物与民族植物学湖南省高校重点实验室，湖南怀化 418008
  • 收稿日期:2011-08-04 修回日期:2011-12-19 出版日期:2012-04-12 网络出版日期:2012-02-13
  • 基金资助:

    本研究由湖南省自然科学基金项目(09JJ3063)资助。

Karyotype Analysis of Arachis hypogaea L. Using Fluorescence Banding and Fluorescence in situ Hybridization with rDNA Probes

SHE Chao-Wen1,2,3,ZHANG Li-Hua1,JIANG Xiang-Hui1,2,3   

  1. 1 Department of Life Sciences, Huaihua University, Huaihua 418008, China; 2 Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua 418008, China; 3 Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua 418008, China
  • Received:2011-08-04 Revised:2011-12-19 Published:2012-04-12 Published online:2012-02-13

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1343

被引次数

10

摘要： 建立花生准确而详细的核型对于阐明其起源和开展其基因组研究十分重要。本研究采用DAPI显带和5S、45S rDNA探针双色荧光原位杂交对花生有丝分裂中期染色体进行了分析。结果表明，花生的单倍基因组总长度为(81.06±3.74) μm，最长染色体为(4.72±0.15) μm，最短染色体为(2.62±0.14)μm；有15对染色体显示了着丝粒区DAPI+带，其中10对为强带，5对为弱带；有2对5S rDNA位点和5对45S rDNA位点，其中1对5S与1对45S位点同线。综合染色体测量数据、DAPI+带和rDNA杂交信号，对花生染色体进行了准确配对和排列，建立了详细的分子细胞遗传学核型。花生的核型公式为2n=4x=40=38m+2sm(SAT)，核型不对称类型属于2A型。

关键词: 花生, 核型, 荧光显带, rDNA, 荧光原位杂交

Abstract: The establishment of an exact and detailed karyotype of Arachis hypogaea L. was fundamental for clarification of the origin and research of the genome of the species. In this study, the mitotic metaphase chromosomes of the species were analyzed using DAPI banding and double fluorescence in situ hybridization (FISH) with 5S and 45S rDNA probes. The mean haploid karyotype length was (81.06±3.74) μm, the longest chromosome pair was (4.72±0.15) μm and the shortest chromosome pair was (2.62±0.14)μm. In the complements of the species, fifteen pairs of the chromosomes displayed centromeric DAPI+ bands including ten pairs of strong bands and five pairs of weak bands; and two pairs of 5S and five pairs of 45S rDNA sites were showed, with one 5S site being syntenic to a 45S site. Combining the chromosome measurements with DAPI+ bands and rDNA FISH signals, the chromosomes were exactly paired and arranged, and a detailed molecular cytogenetic karyotype of A. hypogaea is established. The karyotype formula of A. hypogaea was 2n=4x=40=38m+2sm (SAT) and the asymmetric karyotype belonged to 2A type.

Key words: Arachis hypogaea, Karyotype, Fluorochrome banding, rDNA, Fluorescence in situ hybridization

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