作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1391-1399.doi: 10.3724/SP.J.1006.2013.01391
顾超1,李超1,李晓波2,肖向文2,崔百明1,黄先忠1,*
GU Chao1,LI Chao1,LI Xiao-Bo2,XIAO Xiang-Wen2,CUI Bai-Ming1,HUANG Xian-Zhong1,*
摘要:
通过RT-PCR和RACE技术,从新疆海岛棉品种新海14中克隆得到了一个MFT (MOTHER OF FT AND TFL1)类似基因,命名为GbMFT1基因(GenBank登录号为KC513744)。GbMFT1基因的开放阅读框(ORF)为528 bp,编码175个氨基酸的蛋白,含一个磷脂酰乙醇胺结合蛋白(PEBP)结构域。GbMFT1蛋白的羧基端含有MFT蛋白都含有的脯氨酸。系统进化树分析表明GbMFT1编码产物与葡萄、番茄亲缘关系较近,属于同一进化分枝。实时荧光定量PCR分析表明,GbMFT1基因在棉花的不同组织中均有表达,在花瓣中的表达量较高;在纤维发育的不同时期中均有表达,在开花后2 d的胚珠、9 d的纤维中表达量最高。半定量RT-PCR结果表明,GbMFT1基因在刚萌发的种子中表达量高,用不同浓度的ABA处理种子后其表达变化不明显,表明GbMFT1基因的表达不受ABA的调节。
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