作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1391-1399.doi: 10.3724/SP.J.1006.2013.01391
顾超1,李超1,李晓波2,肖向文2,崔百明1,黄先忠1,*
GU Chao1,LI Chao1,LI Xiao-Bo2,XIAO Xiang-Wen2,CUI Bai-Ming1,HUANG Xian-Zhong1,*
摘要:
通过RT-PCR和RACE技术,从新疆海岛棉品种新海14中克隆得到了一个MFT (MOTHER OF FT AND TFL1)类似基因,命名为GbMFT1基因(GenBank登录号为KC513744)。GbMFT1基因的开放阅读框(ORF)为528 bp,编码175个氨基酸的蛋白,含一个磷脂酰乙醇胺结合蛋白(PEBP)结构域。GbMFT1蛋白的羧基端含有MFT蛋白都含有的脯氨酸。系统进化树分析表明GbMFT1编码产物与葡萄、番茄亲缘关系较近,属于同一进化分枝。实时荧光定量PCR分析表明,GbMFT1基因在棉花的不同组织中均有表达,在花瓣中的表达量较高;在纤维发育的不同时期中均有表达,在开花后2 d的胚珠、9 d的纤维中表达量最高。半定量RT-PCR结果表明,GbMFT1基因在刚萌发的种子中表达量高,用不同浓度的ABA处理种子后其表达变化不明显,表明GbMFT1基因的表达不受ABA的调节。
| [1]Bradley D, Carpenter R, Copsey L, Vincent C, Rothstein S, Coen E. Control of in?orescence architecture in Antirrhinum. Nature, 1996, 379: 791–797[2]Bradley D, Ratcliffe O, Vincent C, Carpenter R, Coen E. In?orescence commitment and architecture in Arabidopsis. Science, 1997, 275: 80–83 [3]Kardailsky I, Shukla V, Ahn J, Dagenais N, Christensen S, Nguyen J, Chory J, Harrison M, Weigel D. Activation tagging of the ?oral inducer FT. Science, 1999, 286: 1962–1965[4]Kobayashi Y, Kaya H, Goto K, Iwabuchi M, Araki T. A pair of related genes with antagonistic roles in mediating ?owering signals. Science, 1999, 286: 1960–1962[5]Hanzawa Y, Money T, Bradley D. A single amino acid converts a repressor to an activator of flowering. Proc Natl Acad Sci USA, 2005, 102: 7748–7753[6]Ahn J H, Miller D, Winter V J, Banfield M J, Lee J H, Yoo S Y, Henz S R , Brady R L, Weigel D. A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO J, 2006, 25: 605–614[7]Ohshima S, Murata M, Sakamoto W, Ogura Y, Motoyoshi F. Cloning and molecular analysis of the Arabidopsis gene Terminal Flower 1. Mol Gen Genet, 1997, 254: 186–194[8]Mimida N, Goto K, Kobayashi Y, Araki T, Ahn J H, Weigel D, Murata M, Motoyoshi F, Sakamoto W. Functional divergence of the TFL1-like gene family in Arabidopsis revealed by characterization of a novel homologue. Genes Cells, 2001, 6: 327–336[9]Yoo S Y, Kardailsky I, Lee J S, Weigel D, Ahn J H. Acceleration of ?owering by overexpression of MFT (MOTHER OF FT AND TFL1). Mol Cells, 2004, 17: 95–101[10]Yamaguchi A, Kobayashi Y, Goto K, Abe M, Araki T. TWIN SISTER OF FT (TSF) acts as a floral pathway integrator redundantly with FT. Plant Cell Physiol, 2005, 46: 1175–1189[11]Corbesier L, Vincent C, Jang S, Fornara F, Fan Q, Searle I, Glakountis A, Farrona S, Gissot L, Turnbul C, Coupland G. FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science, 2007, 316: 1030–1033[12]Jaeger K E, Wigge P A. FT protein acts as a long-range signal in Arabidopsis. Curr Biol, 2007, 17: 1050–1054[13]Zeevaart J A D. Leaf-produced ?oral signals. Curr Opin Plant Biol, 2008, 11: 541–547[14]Tamaki S, Matsuo S, Wong H, Yokoi S, Shimamoto K. Hd3a protein is a mobile flowering signal in rice. Science, 2007, 316:1033–1036[15]Taoka K, Ohki I, Tsuji H, Furuita K, Hayashi K, Yanase T, Yamaguchi M, Nakashima C, Purwestri Y A, Tamaki S, Ogaki Y, Shimada C, Nakagawa A, Kojima C, Shimamoto K. 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen. Nature, 2011, 476: 332–335[16]Navarro C, Abelenda J A, Cruz-Oró E, Cuéllar C A, Tamaki S, Silva J, Shimamoto K, Prat S. Control of flowering and storage organ formation in potato by FLOWERING LOCUS T. Nature, 2011, 478: 119–121[17]Ruonala R, Rinne P L H, Kangasjärvi J, van der Schoot C. CENL1 expression in the rib meristem affects stem elongation and the transition to dormancy in Populus. Plant Cell, 2008, 20: 59–74[18]Böhlenius H, Huang T, Charbonnel-Campaa L, Brunner A M, Jansson S, Strauss S H, Nilsson O. CO/FT regulatory module controls timing of ?owering and seasonal growth cessation in trees. Science, 2006, 312: 1040–1043[19]Lifschitz E, Eviatar T, Rozman A, Shalit A, Goldshmidt A, Amsellem Z, Alvarez J P, Eshed Y. The tomato FT ortholog triggers systemic signals that regulate growth and ?owering and substitute for diverse environmental stimuli. Proc Natl Acad Sci USA, 2006, 103: 6398–6403[20]Hedman H, Källman T, Lagercrantz U. Early evolution of the MFT-like gene family in plants. Plant Mol Biol, 2009, 70: 359–369[21]Gubler F, Millar A A, Jacobsen J V. Dormancy release, ABA and pre-harvest sprouting. Curr Opin Plant Biol, 2005, 8: 183–187[22]Xi W Y, Liu C, Hou X L, Yu H. MOTHER OF FT AND TFL1 regulates seed germination through a negative feedback loop modulating ABA signaling in Arabidopsis. Plant Cell, 2010, 22: 1733–1748[23]Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T, Utsugi S, Ogawa T, Handa H, Ishida H, Mori M, Kawaura K, Ogihara Y, Miura H. A wheat homolog of MOTHER OF FT AND TFL1 acts in the regulation of germination. Plant Cell, 2010, 23: 3215–3229[24]Argiriou A, Michailidis G, Tsaftaris A S. Characterization and expression analysis of TERMINAL FLOWER1 homologs from cultivated alloteraploid cotton (Gossypium hirsutum) and its diploid progenitors. J Plant Physiol, 2008, 165: 1636–1646[25]Dong R(东锐), Yuan H-Y(院海英), Gu C(顾超), Zheng Y-Y(郑银英), Huang X-Z(黄先忠), Cui B-M(崔百明). Clone and primary analysis of the function of GhFTL1 gene in cotton (Gossypium hirsutum). Cotton Sci (棉花学报), 2011, 23(6): 515–521 (in Chinese with English abstract)[26]Hu G-H(胡根海), Yu S-X(喻树迅). Extraction of high-quality total RNA in cotton leaf with improved CTAB method. Cotton Sci (棉花学报), 2007, 19(1): 69–70 (in Chinese with English abstract)[27]Kumar S, Nei M, Dudley. MEGA: a Biologist-centric software for evolutionary of DNA and protein sequences. Briefings Bioninform, 2008, 9: 299–306[28]Reiersen H, Rees A R. The hunchback and its neighbours: proline as an environmental modulator. Trends Biochem Sci, 2001, 26: 679–684[29]Chardon F, Damerval C. Phylogenomic analysis of the PEBP gene family in cereals. J Mol Evol, 2005, 61: 579–590[30]Carmel-Goren L, Liu Y S, Lifschitz E, Zamir D. The SELF-PRUNING gene family in tomato. Plant Mol Biol, 2003, 52: 1215–1222[31]Carmona M J, Calonje M, Martínez-Zapater J M. The FT/TFL1 gene family in grapevine. Plant Mol Biol, 2007, 63: 637–650[32]Igasaki T, Watanabe Y, Nishiguchi M, Kotoda N. The FLOWER LOCUS T/TERMINAL FLOWER 1 family in Lombardy poplar. Plant Cell Physiol, 2008, 49: 291–300[33]Danilevskaya O N, Meng X, Hou Z, Ananiev E V, Simmons C R. A genomic and expression compendium of the expanded PEBP gene family from maize. Plant Physiol, 2008, 146: 250–264[34]Sun H-B(孙洪波), Jia Z(贾贞), Han T-F(韩天富). Roles of PEBP family genes in the development of plants. Plant Physiol J (植物生理学通讯), 2009, 45(8): 739–747 (in Chinese with English abstract)[35]Karlgren A, Gyllenstrand N, Kallman T, Sundstrom J F, Moore D, Lascoux M, Lagercrantz U. Evolution of the PEBP gene family in plants: functional diversi?cation in seed plant evolution. Plant Physiol, 2011, 156: 1967–1977 |
| [1] | 左同鸿, 张贺翠, 曾静, 朱利泉. 甘蓝自交不亲和相关基因BoPUB3L的克隆与表达分析[J]. 作物学报, 2026, 52(6): 1698-1710. |
| [2] | 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912. |
| [3] | 田立涛, 丁宁, 王树林, 齐恩芳, 张荣, 王蕊蕊, 马利雯, 李建武, 杨江伟. 马铃薯Argonaute基因家族鉴定及晚疫病诱导表达分析[J]. 作物学报, 2026, 52(4): 1116-1126. |
| [4] | 王楚锐, 李开祥, 赵志, 肖麓, 唐国永, 赵志刚, 徐亮, 杜德志, 柳海东. 甘蓝型春油菜早花基因BnCRY2功能位点KASP标记的开发及应用[J]. 作物学报, 2026, 52(3): 708-721. |
| [5] | 杨宗桃, 杨婷, 王禹童, 艾静, 李燕烨, 刘家勇, 邓军, 赵勇, 张跃彬. 甘蔗CLC基因家族鉴定与表达分析[J]. 作物学报, 2026, 52(3): 722-734. |
| [6] | 孟成, 王哲. 玉米ZmPFK基因家族全基因组鉴定及响应胁迫表达分析[J]. 作物学报, 2026, 52(3): 764-779. |
| [7] | 张力岚, 杨军, 王让剑. 基于WGCNA发掘茶树糖苷类香气前体含量性状相关的候选基因[J]. 作物学报, 2026, 52(2): 494-513. |
| [8] | 王彬, 蒙姜宇, 邱浩良, 贺亚军, 钱伟. 甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析[J]. 作物学报, 2025, 51(8): 2100-2110. |
| [9] | 郭腾达, 崔梦杰, 陈琳杰, 韩锁义, 郭敬坤, 吴晨迪, 付留洋, 黄冰艳, 董文召, 张新友. 花生磷脂酰肌醇转运蛋白基因AhSFH的克隆及其响应黄曲霉菌侵染的表达特征分析[J]. 作物学报, 2025, 51(6): 1489-1500. |
| [10] | 潘炬忠, 韦萍, 朱德平, 邵胜雪, 陈珊珊, 韦雅倩, 高维维. 水稻转录因子OsERF104的克隆和功能研究[J]. 作物学报, 2025, 51(4): 900-913. |
| [11] | 祁稼民, 许春苗, 肖斌. 马铃薯TIFY基因家族的全基因组鉴定及表达分析[J]. 作物学报, 2024, 50(9): 2297-2309. |
| [12] | 刘震, 陈丽敏, 李志涛, 朱金勇, 王玮璐, 齐喆颖, 姚攀锋, 毕真真, 孙超, 白江平, 刘玉汇. 马铃薯ARM基因家族的全基因组鉴定及表达分析[J]. 作物学报, 2024, 50(6): 1451-1466. |
| [13] | 曹松, 姚敏, 任睿, 贾元, 向星汝, 李文, 何昕, 刘忠松, 官春云, 钱论文, 熊兴华. 转录组结合区域关联分析挖掘油菜含油量积累的候选基因[J]. 作物学报, 2024, 50(5): 1136-1146. |
| [14] | 李海芬, 鲁清, 刘浩, 温世杰, 王润风, 黄璐, 陈小平, 洪彦彬, 梁炫强. 花生赤霉素3-β-双加氧酶(AhGA3ox)基因家族的全基因组鉴定及表达分析[J]. 作物学报, 2024, 50(4): 932-943. |
| [15] | 代洪苇, 刘洁强, 张丽, 童华荣, 袁连玉. 茶树CsMCC1和CsMCC2基因的克隆及表达特征性分析[J]. 作物学报, 2024, 50(3): 656-668. |
|
||