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作物学报 ›› 2025, Vol. 51 ›› Issue (8): 2100-2110.doi: 10.3724/SP.J.1006.2025.55007

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析

王彬**(), 蒙姜宇**(), 邱浩良, 贺亚军*(), 钱伟   

  1. 西南大学农学与生物科技学院, 重庆 400716
  • 收稿日期:2025-01-15 接受日期:2025-04-27 出版日期:2025-08-12 网络出版日期:2025-05-26
  • 通讯作者: *贺亚军, E-mail: hyj790124@163.com
  • 作者简介:王彬, E-mail: 1666842119@qq.com;
    蒙姜宇, E-mail: 13002363008@163.com

    **同等贡献

  • 基金资助:
    国家自然科学基金项目(32272060);国家重点研发计划项目(2022YFD1200400);重庆市自然科学基金(cstc2021jcyj-msxmx1198);重庆市自然科学基金(CSTB2024NSCQ-MSX0423)

Identification and expression pattern analysis of the BnaDUF579 gene family in Brassica napus

WANG Bin**(), MENG Jiang-Yu**(), QIU Hao-Liang, HE Ya-Jun*(), QIAN Wei   

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
  • Received:2025-01-15 Accepted:2025-04-27 Published:2025-08-12 Published online:2025-05-26
  • Contact: *E-mail: hyj790124@163.com
  • About author:

    **Contributed equally to this work

  • Supported by:
    National Natural Science Foundation of China(32272060);National Key Research and Development Program of China(2022YFD1200400);Natural Science Foundation of Chongqing(cstc2021jcyj-msxmx1198);Natural Science Foundation of Chongqing(CSTB2024NSCQ-MSX0423)

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摘要：

Domain of unknown function 579 (DUF579)家族广泛存在于真核生物中, 该家族在次生细胞壁发育和木聚糖生物合成中发挥着重要的作用。目前, 还没有研究系统报道过甘蓝型油菜BnaDUF579基因家族。本研究通过生物信息学方法对甘蓝型油菜BnaDUF579基因家族进行进化树构建、基因结构和保守基序分析、染色体分布和共线性分析、组织表达分析、启动子顺式作用元件分析。结果表明, 甘蓝型油菜BnaDUF579家族成员有31个, 其中的24个成员都只有1个外显子、没有内含子。这些基因分为4个分支, 包括Group1、Group2、Group3和Group4。同一分支内的成员具有相似的基序组成, 但不同分支之间的成员在基序组成上存在明显差异。该家族与白菜亲缘关系较远、与甘蓝亲缘关系更近。BnaDUF579基因家族主要在油菜茎、根、角果和种子中表达, 其启动子顺式作用元件涉及激素反应、对非生物胁迫的反应、组织发育、光响应等生理过程。以上研究结果丰富了对甘蓝型油菜BnaDUF579基因的认识, 为油菜BnaDUF579基因的功能研究奠定了基础。

关键词: 甘蓝型油菜, BnaDUF579, 基因家族, 表达分析, 木聚糖

Abstract:

The domain of unknown function 579 (DUF579) family is widely distributed across eukaryotes and plays a critical role in secondary cell wall development and xylan biosynthesis. However, a comprehensive investigation of BnaDUF579 genes in Brassica napus has not yet been reported. In this study, we performed a genome-wide identification and bioinformatic analysis of BnaDUF579 family members. Phylogenetic relationships, gene structure, conserved motif composition, chromosomal distribution, and collinearity were systematically analyzed. Additionally, tissue-specific expression patterns and promoter cis-acting elements were examined. A total of 31 BnaDUF579 genes were identified, of which 24 contained only a single exon. Based on sequence alignment and phylogenetic analysis, these genes were classified into four clades, Group1, Group2, Group3, and Group4. Genes within the same clade exhibited similar motif compositions, whereas those in different clades showed distinct differences. Evolutionary analysis revealed that the BnaDUF579 gene family is more closely related to that of B. oleracea than to B. rapa. Expression profiling showed that BnaDUF579 genes are predominantly expressed in the stem, root, silique, and seed tissues of rapeseed. Promoter analysis indicated that cis-acting elements associated with hormone responses, abiotic stress, tissue development, and light responsiveness are widely present. Overall, these findings enhance our understanding of the BnaDUF579 gene family and provide a foundation for future functional studies in Brassica napus.

Key words: Brassica napus, BnaDUF579, gene family, expression analysis, xyloglucan

表1

本研究中用到的qRT-PCR引物"

基因名称
Gene name		 正向引物序列
Forward primer sequence (5′-3′)		 反向引物序列
Reverse primer sequence (5′-3′)
BnaActin7 GGAGCTGAGAGATTCCGTTG GAACCACCACTGAGGACGAT
BnaA09G0469400ZS CCTTCCCCAACCACCACT CGAAGACGAGGAGGTTGC
BnaA07G0280400ZS ACTTTCGCGGAGGAGTTCTT CCTGTAATCTCCACCGTCG
BnaC06G0382400ZS CGGCGGCTAGAGATAGAGAA GCTTCTTCGTGAAACCCAGTA
BnaC07G0206400ZS CTTCACCATCGCTTTTCTCCT CGTTGGCAACTGAGAGGTG
BnaA09G0107100ZS CGTCGACGAGAATCCTTACTTA GTTAGGTAAATCGTTGATCGCC

表2

甘蓝型油菜BnaDUF579家族成员基本信息表"

数目
Number		 分支
Group		 基因名称
Gene ID		 蛋白长度
Protein length (aa)		 分子量
Molecular weight (Da)		 等电点
Isoelectric point
1 Group1 BnaC06G0104800ZS 296 33,691.33 8.03
2 Group1 BnaA09G0381200ZS 296 33,692.32 7.59
3 Group1 BnaA08G0293400ZS 281 31,840.50 7.57
4 Group1 BnaA05G0318700ZS 297 33,747.22 6.51
5 Group1 BnaC08G0195300ZS 281 31,835.56 8.35
6 Group1 BnaC06G0382400ZS 296 33,018.62 5.94
7 Group1 BnaA07G0326600ZS 296 33,077.72 6.31
8 Group1 BnaC02G0257800ZS 106 12,216.96 5.60
9 Group1 BnaC02G0257900ZS 163 17,749.29 5.13
10 Group2 BnaA03G0394800ZS 327 35,772.35 6.00
11 Group2 BnaA09G0469400ZS 324 36,052.89 6.60
12 Group2 BnaC08G0304500ZS 324 36,080.95 6.60
13 Group2 BnaC07G0206400ZS 315 35,596.34 7.18
14 Group2 BnaA07G0142200ZS 315 35,637.43 7.16
15 Group2 BnaC09G0093600ZS 308 34,733.51 6.82
16 Group2 BnaC03G0489400ZS 330 36,106.81 6.37
17 Group2 BnaA09G0107100ZS 323 35,524.20 7.12
18 Group2 BnaC09G0108200ZS 320 35,198.92 6.75
19 Group3 BnaC03G0580500ZS 139 15,392.55 8.93
20 Group3 BnaA07G0097300ZS 289 32,282.19 8.53
21 Group3 BnaC07G0148300ZS 289 32,328.22 8.52
22 Group3 BnaC06G0321200ZS 291 32,547.31 6.45
23 Group3 BnaA07G0280400ZS 291 32,535.25 6.24
24 Group3 BnaA09G0427700ZS 289 32,399.37 9.16
25 Group3 BnaC05G0239000ZS 289 32,399.37 9.16
26 Group3 BnaA02G0165700ZS 288 32,293.04 8.17
27 Group3 BnaC02G0212300ZS 295 33,182.18 8.67
28 Group4 BnaC07G0460400ZS 312 34,769.71 9.63
29 Group4 BnaC01G0183700ZS 311 34,904.00 9.57
30 Group4 BnaA01G0144700ZS 309 34,794.00 9.70
31 Group4 BnaA03G0482400ZS 312 34,980.20 9.74

图1

甘蓝型油菜、拟南芥、白菜型油菜和甘蓝中DUF579蛋白的系统进化分析"

图2

甘蓝型油菜BnaDUF579基因家族的保守基序、保守结构域和基因结构 A: BnaDUF579蛋白的系统进化分析; B: 保守基序分析; C: BnaDUF579的保守结构域; D: BnaDUF579基因结构。"

图3

甘蓝型油菜BnaDUF579基因在染色体上的分布"

图4

甘蓝型油菜与拟南芥、白菜和甘蓝DUF579基因的共线性关系 红色线条表示甘蓝型油菜BnaDUF579基因与拟南芥、甘蓝的直系同源基因对应关系, 黑色线条表示甘蓝型油菜BnaDUF579基因与白菜的直系同源基因对应关系。"

图5

BnaDUF579基因在甘蓝型油菜不同组织和器官的表达模式 A: 转录组数据中BnaDUF579基因的表达模式。B: 通过qRT-PCR验证部分BnaDUF579基因的表达量。"

图6

BnaDUF579基因的启动子顺式作用元件分析"

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