甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析

doi:10.3724/SP.J.1006.2025.55007

摘要/Abstract

摘要： Domain of unknown function 579 (DUF579)家族广泛存在于真核生物中，该家族在次生细胞壁发育和木聚糖生物合成中发挥着重要的作用。目前，还没有研究系统报道过甘蓝型油菜BnaDUF579基因家族。本研究通过生物信息学方法对甘蓝型油菜BnaDUF579基因家族进行进化树构建、基因结构和保守基序分析、染色体分布和共线性分析、组织表达分析、启动子顺式作用元件分析。结果表明，有31个甘蓝型油菜BnaDUF579家族成员得到鉴定，其中的24个成员都只有一个外显子、没有内含子。这些基因分为4个分支，包括Group1、Group2、Group3和Group4。同一分支内的成员具有相似的基序组成，但不同分支之间的成员在基序组成上存在明显差异。该家族与白菜亲缘关系较远、与甘蓝亲缘关系更近。BnaDUF579基因家族主要在油菜茎、根、角果和种子中表达，其启动子顺式作用元件涉及激素反应、对非生物胁迫的反应、组织发育、光响应。以上研究结果丰富了对甘蓝型油菜BnaDUF579基因的认识，为油菜BnaDUF579基因的功能研究奠定了基础。

关键词: 甘蓝型油菜, BnaDUF579, 基因家族, 表达分析, 木聚糖

Abstract:

The Domain of Unknown Function 579 (DUF579) family is widely distributed across eukaryotes and plays a critical role in secondary cell wall development and xylan biosynthesis. However, a comprehensive investigation of BnaDUF579 genes in Brassica napus has not yet been reported. In this study, we performed a genome-wide identification and bioinformatic analysis of BnaDUF579 family members. Phylogenetic relationships, gene structure, conserved motif composition, chromosomal distribution, and collinearity were systematically analyzed. Additionally, tissue-specific expression patterns and promoter cis-acting elements were examined. A total of 31 BnaDUF579 genes were identified, of which 24 contained only a single exon. Based on sequence alignment and phylogenetic analysis, these genes were classified into four clades: Group 1, Group 2, Group 3, and Group 4. Genes within the same clade exhibited similar motif compositions, whereas those in different clades showed distinct differences. Evolutionary analysis revealed that the BnaDUF579 gene family is more closely related to that of B. oleracea than to B. rapa. Expression profiling showed that BnaDUF579 genes are predominantly expressed in the stem, root, silique, and seed tissues of rapeseed. Promoter analysis indicated that cis-acting elements associated with hormone responses, abiotic stress, tissue development, and light responsiveness are widely present. Overall, these findings enhance our understanding of the BnaDUF579 gene family and provide a foundation for future functional studies in Brassica napus.

Key words: Brassica napus, DUF579, gene family, expression analysis, xyloglucan

王彬, 蒙姜宇, 邱浩良, 贺亚军, 钱伟. 甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析[J]. 作物学报, 0, (): 0-.

WANG Bin, MENG Jiang-Yu, QIU Hao-Liang, HE Ya-Jun, QIAN Wei. Identification and expression pattern analysis of the BnaDUF579 gene family in Brassica napus[J]. Acta Agronomica Sinica, 0, (): 0-.

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