作物学报 ›› 2024, Vol. 50 ›› Issue (8): 1934-1947.doi: 10.3724/SP.J.1006.2024.32046
何丹丹1,2(), 舒亚洲1,2(
), 周海连1,2, 吴松果1,2, 魏晓双1,2, 杨明冲1,2, 李波1,2, 吴正丹1,2, 韩世健2, 杨娟2,3, 王继斌1,2,4,*(
), 王令强1,2,3,*(
)
HE Dan-Dan1,2(), SHU Ya-Zhou1,2(
), ZHOU Hai-Lian1,2, WU Song-Guo1,2, WEI Xiao-Shuang1,2, YANG Ming-Chong1,2, LI Bo1,2, WU Zheng-Dan1,2, HAN Shi-Jian2, YANG Juan2,3, WANG Ji-Bin1,2,4,*(
), WANG Ling-Qiang1,2,3,*(
)
摘要:
RPTA (regulatory particle triple-A ATPase)家族与植物的生长发育、激素调控和逆境胁迫反应密切相关。本研究一共鉴定到33个OsRPTA基因家族成员, 并分析了其基因位置、基因结构、motif组成和启动子顺式作用元件等信息。随后, 利用水稻CREP数据库下载的数据, 分析了OsRPTA基因家族成员的全生育期组织表达模式。发现大部分OsRPTA基因在穗、胚乳和愈伤组织中具有较高的表达水平。β-D-葡萄糖苷酸酶(GUS)染色进一步显示, 基因成员OsRPTA18主要在叶枕、根、叶、叶鞘、茎节、内稃和外稃的维管束等部位表达。亚细胞定位结果显示OsRPTA18蛋白定位于细胞核。通过CRISPR/Cas9基因编辑获得了突变体材料osrpta18-1和osrpta18-2。与中花11相比, 突变体植株的株高、叶倾角变小, 粒宽和千粒重降低。组织切片结果表明, 突变体osrpta18旗叶倾角变小是由于叶枕近轴面厚壁细胞增殖, 导致近轴面与远轴面细胞和维管束的不对称性减弱。本研究有助于了解水稻RPTA基因家族功能, 并为利用OsRPTA18基因培育理想株型的水稻品种提供参考。
[1] | Chen L T, Li Y C, Sottas C, Lazaris A, Petrillo S K, Metrakos P, Li L, Ishida Y, Saito T, Garza S, Papadopoulos V. Loss of mitochondrial ATPase ATAD3A contributes to nonalcoholic fatty liver disease through accumulation of lipids and damaged mitochondria. J Biol Chem, 2022, 298: 102008. |
[2] |
Xu H Y, Holly V R. The SarcoEndoplasmic Reticulum Calcium ATPase (SERCA) pump: a potential target for intervention in aging and skeletal muscle pathologies. Skelet Muscle, 2021, 11: 25.
doi: 10.1186/s13395-021-00280-7 pmid: 34772465 |
[3] |
Kotani Y, Morito D, Yamazaki S, Ogino K, Kawakami K, Takashima S, Hirata H, Nagata K. Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213. Sci Rep, 2015, 5: 16161.
doi: 10.1038/srep16161 pmid: 26530008 |
[4] |
Morito D, Nishikawa K, Hoseki J, Kitamura A, Kotani Y, Kiso K, Kinjo M, Fujiyoshi Y, Nagata K. Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state. Sci Rep, 2014, 4: 4442.
doi: 10.1038/srep04442 pmid: 24658080 |
[5] |
Qi Y, Liu H, Daniels M P, Zhang G, Xu H. Loss of Drosophila i-AAA protease, dYME1L, causes abnormal mitochondria and apoptotic degeneration. Cell Death Differ, 2016, 23: 291-302.
doi: 10.1038/cdd.2015.94 pmid: 26160069 |
[6] | Cui K, Qin L, Tang X Y, Nong J K, Chen J, Wu N, Gong X, Yi L X, Yang C H, Xia S T. A single amino acid substitution in RFC4 leads to endoduplication and compromised resistance to DNA damage in Arabidopsis thaliana. Genes, 2022, 13: 1037. |
[7] | Zhang B T, Van Aken O, Thatcher L, De Clercq I, Duncan O, Law S R, Murcha M W, van der Merwe M, Seifi H S, Carrie C, Cazzonelli C, Radomiljac J, Höfte M, Singh K B, Van Breusegem F, Whelan J. The mitochondrial outer membrane AAA ATPase AtOM66 affects cell death and pathogen resistance in Arabidopsis thaliana. Plant J, 2014, 80: 709-727. |
[8] |
Wu Q F, Han T T, Yang L, Wang Q, Zhao Y X, Jiang D, Ruan X. The essential roles of OsFtsH2 in developing the chloroplast of rice. BMC Plant Biol, 2021, 21: 445.
doi: 10.1186/s12870-021-03222-z pmid: 34598671 |
[9] | 张海丽, 高静, 张昊, 李生辉, 邢继红, 王凤茹, 董金皋. 油菜素内酯对水稻细胞伸长和分裂的调控. 农业生物技术学报, 2015, 23: 71-79. |
Zhang H L, Gao J, Zhang H, Li S H, Xing J H, Wang F R, Dong J G. The regulation of brassinosteroid (BR) on elongation and division of rice (Oryza sativa) cells. J Agric Biotechnol, 2015, 23: 71-79 (in Chinese with English abstract). | |
[10] | Guo J F, Li W, Shang L G, Wang Y G, Yan P, Bai Y H, Da X W, Wang K, Guo Q Q, Jiang R R, Mao C Z, Mo X R. OsbHLH98 regulates leaf angle in rice through transcriptional repression of OsBUL1. New Phytol, 2021, 230: 1953-1966. |
[11] | Huang Q N, Shi Y F, Zhang X B, Song L X, Feng B H, Wang H M, Xu X, Li X H, Guo D, Wu J L. Single base substitution in OsCDC48isresponsible for premature senescence and death phenotype in rice. J Integr Plant Biol, 2016, 58: 12-28. |
[12] | Zhang Y, Iqbal M F, Wang Y L, Qian K Y, Xiang J X, Xu G H, Fan X R. OsTBP2.1, A TATA-binding protein, alters the ratio of OsNRT2.3b to OsNRT2.3a and improves rice grain yield. Int J Mol Sci, 2022, 23: 10795. |
[13] | Yedidi R S, Wendler P, Enenkel C. AAA-ATPases in protein degradation. Int J Mol Sci, 2017, 4: 42. |
[14] |
Tong H N, Chu C C. Functional specificities of brassinosteroid and potential utilization for crop improvement. Trends Plant Sci, 2018, 23: 1016-1028.
doi: S1360-1385(18)30187-0 pmid: 30220494 |
[15] |
许娜, 徐铨, 徐正进, 陈温福. 水稻株型生理生态与遗传基础研究进展. 作物学报, 2023, 49: 1735-1746.
doi: 10.3724/SP.J.1006.2023.22050 |
Xu N, Xu Q, Xu Z J, Chen W F. Research progress on physiological ecology and genetic basis of rice plant architecture. Acta Agron Sin, 2023, 49: 1735-1746 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2023.22050 |
|
[16] |
马梦影, 巩文靓, 康雪蒙, 段海燕. 水稻理想株型改良的研究进展. 中国农学通报, 2020, 36(29): 1-6.
doi: 10.11924/j.issn.1000-6850.casb20190900610 |
Ma M Y, Gong W Y, Kang X M, Duan H Y. The improvement of ideal plant type of rice: a review. Chin Agric Sci Bull, 2020, 36(29): 1-6 (in Chinese with English abstract).
doi: 10.11924/j.issn.1000-6850.casb20190900610 |
|
[17] | Dong H J, Zhao H, Li S L, Han Z M, Hu G, Liu C, Yang G Y, Wang G W, Xie W B, Xing Y Z. Genome-wide association studies reveal that members of bHLH subfamily 16 share a conserved function in regulating flag leaf angle in rice (Oryza sativa L.). PLoS Genet, 2018, 14: 1007323. |
[18] | 徐静, 王莉, 钱前, 张光恒. 水稻叶片形态建成分子调控机制研究进展. 作物学报, 2013, 39: 767-774. |
Xu J, Wang L, Qian Q, Zhang G H. Research advance in molecule regulation mechanism of leaf morphogenesis in rice (Oryza sativa L.). Acta Agron Sin, 2013, 39: 767-774 (in Chinese with English abstract). | |
[19] |
胡娟, 林晗, 徐娜, 焦然, 戴志俊, 鲁草林, 饶玉春, 王跃星. 水稻叶倾角分子机制及育种应用的研究进展. 中国水稻科学, 2019, 33: 391-400.
doi: 10.16819/j.1001-7216.2019.9029 |
Hu J, Lin H, Xu N, Jiao R, Dai Z J, Lu C L, Rao Y C, Wang Y X. Advances in molecular mechanisms of rice leaf inclination and its application in breeding. Chin J Rice Sci, 2019, 33: 391-400 (in Chinese with English abstract).
doi: 10.16819/j.1001-7216.2019.9029 |
|
[20] | Huang P, Zhao J Z, Hong J L, Zhu B, Xia S, Zhu E G, Han P F, Zhang K W. Cytokinins regulate rice lamina joint development and leaf angle. Plant Physiol, 2023, 191: 56-69. |
[21] | Xing M Q, Wang W, Fang X, Xue H W. Rice OsIAA6 interacts with OsARF1 and regulates leaf inclination. Crop J, 2022, 10: 1580-1588. |
[22] |
Fàbregas N, Caño-Delgado A I. Turning on the microscope turret: a new view for the study of brassinosteroid signaling in plant development. Physiol Plant, 2014, 151: 172-183.
doi: 10.1111/ppl.12130 pmid: 24547704 |
[23] | 张晓琼, 王晓雯, 田维江, 张孝波, 孙莹, 李杨羊, 谢佳, 何光华, 桑贤春. LAZY1通过BR途径调控水稻叶夹角的发育. 作物学报, 2017, 43: 1767-1773. |
Zhang X Q, Wang X W, Tian W J, Zhang X B, Sun Y, Li Y Y, Xie J, He G H, Sang X C. LAZY1regulates the development of rice leaf angle through BR pathway. Acta Agron Sin, 2017, 43: 1767-1773 (in Chinese with English abstract). | |
[24] |
Gan L J, Wu H, Wu D P, Zhang Z F, Guo Z F, Yang N, Xia K, Zhou X, Oh K, Matsuoka M, Ng D, Zhu C H. Methyl jasmonate inhibits lamina joint inclination by repressing brassinosteroid biosynthesis and signaling in rice. Plant Sci, 2015, 241: 238-245.
doi: 10.1016/j.plantsci.2015.10.012 pmid: 26706074 |
[25] | Chen H J, Wan Y, Teng K C, Liu B H, Zhao N, Xu K Z, Li J X. The role of OsOFP8gene in regulating rice leaf angle. J Plant Biochem Biot, 2023, 32: 304-318. |
[26] | 凌启鸿, 陆卫平, 蔡建中, 曹显祖. 水稻根系分布与叶角关系的研究初报. 作物学报, 1989, 15: 123-131. |
Ling Q H, Lu W P, Cai J Z, Cao X Z. The relationship between root distribution and leaf angle in rice plant. Acta Agron Sin, 1989, 15: 123-131 (in Chinese with English abstract). | |
[27] |
Liu J M, Park S J, Huang J, Lee E J, Xuan Y H, Je BI, Kumar V, Priatama R A, Vimal R K, Kim S H, Min M K, Cho J H, Kim T H, Chandran A K N, Jung K H, Takatsuto S, Fujioka S, Han C D. Loose Plant Architecture1 (LPA1) determines lamina joint bending by suppressing auxin signalling that interacts with C-22-hydroxylated and 6-deoxo brassinosteroids in rice. J Exp Bot, 2016, 67: 1883-1895.
doi: 10.1093/jxb/erw002 pmid: 26826218 |
[28] |
Luo X Y, Zheng J S, Huang R Y, Huang Y M, Wang H C, Jiang L R, Fang X J. Phytohormones signaling and crosstalk regulating leaf angle in rice. Plant Cell Rep, 2016, 35: 2423-2433.
pmid: 27623811 |
[29] | Kokkirala V R, Yonggang P, Abbagani S, Zhu Z, Umate P. Subcellular localization of proteins of Oryza sativa L. in the model tobacco and tomato plants. Plant Signal Behav, 2010, 5: 1336-1341. |
[30] | Lee J H, Schöffl F. GUS activity staining in gels: a powerful tool for studying protein interactions in plants. Plant Mol Biol Rep, 1995, 13: 346-354. |
[31] | 杨捷频. 常规石蜡切片方法的改良. 生物学杂志, 2006, 23(1): 45-46. |
Yang J P. Improvement of traditional paraffin section preparation methods. J Biol, 2006, 23(1): 45-46 (in Chinese with English abstract). | |
[32] |
Ferrero-Serrano Á, Assmann S M. The α-subunit of the rice heterotrimeric G protein, RGA1, regulates drought tolerance during the vegetative phase in the dwarf rice mutant d1. J Exp Bot, 2016, 67: 3433-3443.
doi: 10.1093/jxb/erw183 pmid: 27194741 |
[33] |
Cao Y Y, Zhong Z J, Wang H Y, Shen R X. Leaf angle: a target of genetic improvement in cereal crops tailored for high-density planting. Plant Biotechnol J, 2022, 20: 426-436.
doi: 10.1111/pbi.13780 pmid: 35075761 |
[1] | 贾舒涵, 何璨, 陈敏, 刘家欣, 胡伟民, 胡晋, 关亚静. 杂交水稻不同穗萌程度种子质量差异与穗萌分级研究[J]. 作物学报, 2024, 50(9): 2310-2322. |
[2] | 胡丽琴, 肖正午, 方升亮, 曹放波, 陈佳娜, 黄敏. 种植季节对高直链淀粉水稻品种淀粉消化特性的影响[J]. 作物学报, 2024, 50(9): 2347-2357. |
[3] | 刘陈, 王昆昆, 廖世鹏, 杨佳群, 丛日环, 任涛, 李小坤, 鲁剑巍. 氮肥用量对玉米-油菜和水稻-油菜轮作模式下油菜产量及氮素吸收利用的影响[J]. 作物学报, 2024, 50(8): 2067-2077. |
[4] | 宋志文, 赵蕾, 毕俊国, 唐清芸, 王国栋, 李玉祥. 滴灌条件下施氮量对不同氮效率水稻品种物质积累及养分吸收的影响[J]. 作物学报, 2024, 50(8): 2025-2038. |
[5] | 邵美红, 赵玲玲, 程楚, 程思明, 朱双兵, 翟来圆, 陈凯, 徐建龙. 水稻黄华占背景选择导入系的耐低氮筛选评价与利用[J]. 作物学报, 2024, 50(8): 1907-1919. |
[6] | 付景, 马梦娟, 张骐飞, 段居琦, 王越涛, 王付华, 王生轩, 白涛, 尹海庆, 王亚. 干湿交替灌溉和施氮量对粳稻光合特性和氮素吸收利用的影响[J]. 作物学报, 2024, 50(7): 1787-1804. |
[7] | 裴法敬, 张文轩, 张晓, 王昕钰, 彭少兵, 米甲明. 长粒香型的超短生育期水稻新品系创制[J]. 作物学报, 2024, 50(7): 1684-1698. |
[8] | 唐清芸, 杨晶晶, 赵蕾, 宋志文, 王国栋, 李玉祥. 施氮量对滴灌水稻根系形态构型和分形特征的影响[J]. 作物学报, 2024, 50(6): 1540-1553. |
[9] | 张小芳, 朱琪, 华芸堰, 贾黎惠莹, 邱士优, 陈宇杰, 马涛, 丁沃娜. 水稻OsCYP22互作蛋白的筛选及验证[J]. 作物学报, 2024, 50(6): 1628-1634. |
[10] | 朱忠林, 文月, 周棋, 巫燕飞, 杜雪竹, 盛锋. 水稻OsCNGC10基因抗倒伏性以及抗旱性功能研究[J]. 作物学报, 2024, 50(5): 1351-1360. |
[11] | 胡明明, 丁峰, 彭志芸, 向开宏, 李郁, 张宇杰, 杨志远, 孙永健, 马均. 多元化种植模式下秸秆还田配合水氮管理对水稻产量形成与氮素吸收利用的影响[J]. 作物学报, 2024, 50(5): 1236-1252. |
[12] | 耿孝宇, 张翔, 刘洋, 左博源, 朱旺, 马唯一, 汪璐璐, 孟天瑶, 高平磊, 陈英龙, 许轲, 戴其根, 韦还和. 江苏省滨海盐碱地籼粳杂交稻产量优势形成特征[J]. 作物学报, 2024, 50(5): 1253-1270. |
[13] | 万应春, 班义结, 蒋钰东, 王亚欣, 刘晶晶, 刘晓晴, 程育林, 王楠, 冯萍. 水稻雄性不育突变体tpa1的表型鉴定与精细定位[J]. 作物学报, 2024, 50(5): 1104-1114. |
[14] | 余瑶, 王紫瑶, 周思睿, 刘鹏程, 叶亚峰, 马伯军, 刘斌美, 陈析丰. 水稻类病变突变体lms1的表型鉴定与抗病分子机制分析[J]. 作物学报, 2024, 50(4): 857-870. |
[15] | 王吕, 吴玉红, 秦宇航, 淡亚彬, 陈浩, 郝兴顺, 田霄鸿. 紫云英稻秸秆协同还田与氮肥减量配施对水稻干物质积累、氮素转运及产量的影响[J]. 作物学报, 2024, 50(3): 756-770. |
|