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作物学报 ›› 2013, Vol. 39 ›› Issue (10): 1835-1842.doi: 10.3724/SP.J.1006.2013.01835

• 耕作栽培·生理生化 • 上一篇    下一篇

外源IAA、GA3和ABA影响不同穗型小麦分蘖发生的机制

蔡铁,徐海成,尹燕枰,杨卫兵,彭佃亮,倪英丽,徐彩龙,杨东清,王振林*   

  1. 山东农业大学作物生物学国家重点实验室,山东泰安 271018
  • 收稿日期:2013-01-21 修回日期:2013-06-01 出版日期:2013-10-12 网络出版日期:2013-07-31
  • 通讯作者: 王振林, E-mail: zlwang@sdau.edu.cn, zlwangsd@sina.com, Tel: 0538-8241359
  • 基金资助:

    本研究由国家自然科学基金项目(31271661, 30871477),国家重点基础研究发展计划(973计划)项目(2009CB118602)和国家公益性行业(农业)科研专项(201203100)资助。

Mechanisms of Tiller Occurrence Affected by Exogenous IAA, GA3, and ABA in Wheat with Different Spike-types

CAI Tie,XU Hai-Cheng,YIN Yan-Ping,YANG Wei-Bing,PENG Dian-Liang,NI Ying-Li,XU Cai-Long,YANG Dong-Qing,WANG Zhen-Lin*   

  1. State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China.
  • Received:2013-01-21 Revised:2013-06-01 Published:2013-10-12 Published online:2013-07-31
  • Contact: 王振林, E-mail: zlwang@sdau.edu.cn, zlwangsd@sina.com, Tel: 0538-8241359

摘要:

以山农15和山农8355为材料,研究外源生长素(IAA)、赤霉素(GA3)、脱落酸(ABA)对小麦分蘖发生的影响及其作用方式。结果显示,外源IAA和GA3可抑制分蘖的发生,而外源ABA则仅减缓了分蘖发生速率。进一步分析外源激素对分蘖芽生长动态及分蘖节中内源激素变化的影响,发现外源IAA和GA3完全抑制分蘖芽的生长,而外源ABA明显减缓了分蘖芽的生长速率。外源IAA提高分蘖节中IAA含量和IAA/ZT比,抑制ZT含量的提高;外源GA3提高分蘖节中IAA含量、IAA/ZT比和ABA/ZT比,抑制ZT含量的提高;外源ABA提高分蘖节中ABA/ZT比,减缓了ZT含量的升高速率。相关性分析显示,分蘖芽的生长与ZT呈显著正相关,与IAA、IAA/ZT比和ABA/ZT比呈显著负相关,与GA3和ABA呈不显著的负相关。试验结果表明,IAA和ZT在小麦分蘖发生过程中起关键作用,外源激素主要通过影响内源IAA、ZT含量以及IAA/ZT比和ABA/ZT比来影响分蘖芽的生长,进而调控小麦分蘖的发生。

关键词: 小麦, 分蘖芽, 植物激素, 调控

Abstract:

Two winter wheat cultivars, Shannong 15 and Shannong 8355, were used to study the effects of exogenous indole-3-acetic acid (IAA), gibberellic acid 3 (GA3), and abscisic acid (ABA) on tiller occurrence in wheat and the underlying mechanism. Exogenous IAA and GA3 inhibited occurrence of wheat tiller, while exogenous ABA reduced the rate of tiller occurrence. Furthermore, exogenous IAA and GA3 completely inhibited growth of tiller bud, while exogenous ABA significantly slowed the growth rate of tiller bud. The growth of tiller bud was positively correlated with content ofzeatin (ZT), but negatively correlated with IAA content and ratios of IAA/ZT and ABA/ZT. In contrast, the growth of tiller bud had slightly negative correlations with GA3 and ABA contents. These results suggest that IAA and ZT play a key role in regulating tiller occurrence in wheat, and exogenous hormones regulate the growth of tiller bud through changing contents of IAA and ZT and the ratios of IAA/ZT and ABA/ZT in tiller node.

Key words: Wheat, Tiller bud, Plant hormones, Regulation

[1]Li X Y, Qian Q, Fu Z M, Wang Y H, Xiong G S, Zeng D L, Wang X Q, Liu X F, Teng S, Hiroshi F, Yuan M, Luo D, Han B, Li J Y. Control of tillering in rice. Nature, 2003, 422: 618−621



[2]Liu Y, Gu D D, Ding Y F, Wang Q S, Li G H, Wang S H. The relationship between nitrogen, auxin and cytokinin in the growth regulation of rice (Oryza sativa L.) tiller buds. Aust J Crop Sci, 2011, 5: 1019–1026



[3]Chatfield S P, Stirnberg P, Forde B G, Leyser O. The hormonal regulation of axillary bud growth in Arabidopsis. Plant J, 2000, 24: 159−169



[4]Shimizu S S, Mori H. Control of outgrowth and dormancy in axillary buds. Plant Physiol, 2001, 127: 1405−1413



[5]Wang G, Romheld V, Li C, Bangerth F. Involvement of auxin and CKs in boron deficiency induced changes in apical dominance of pea plants. J Plant Physiol, 2006, 163: 591−600



[6]Leopold A. The control of tillering in grasses by auxin. Am J Bot, 1949, 36: 437−440



[7]Langer R, Prasad P, Laude H. Effects of kinetin on tiller bud elongation in wheat (Triticum aestivum L.). Ann Bot, 1973, 37: 565−571 



[8]Rood, S. B. Application of gibberellic acid to control tillering in early-maturing maize. Can J Plant Sci, 1985, 65: 901−911



[9]Hong X-F(洪晓富), Jiang P-Y(蒋彭炎), Zheng Z-S(郑寨生), Lu C-Y(卢昌银), Wang C-M(王撮明). Relationships between employ GA3 during tillering stage and promote the panicle bearing tiller rate. J Zhejiang Agric Sci (浙江农业科学), 1998, (1): 3−5 (in Chinese with English abstract)



[10]Zhang G P. Gibberellic acid 3 modifies some growth and physiologic effects of paclobutrazol (PP333) on wheat. J Plant Growth Regul, 1997, 16: 21−25



[11]Phillips I D J. Apocaldominance. Annu Rev J Plant Physiol, 1975, 26: 341−347



[12]Emery R J N, Longnecler N E, Atkins C A. Branch development in Lupinus angustifolius L.: II. Relationship with endogenous ABA, IAA and CKs in axillary and main stem buds. J Exp Bot, 1998, 49: 555−562



[13]Wang R-F(王如芳), Zhang J-W(张吉旺), Lü P(吕鹏), Dong S-T(董树亭), Liu P(刘鹏), Zhao B(赵斌). Effects of endogenous hormones on tiller development process of different maize varieties. Sci Agric Sin (中国农业科学), 2012, 45(5): 840−847 (in Chinese with English abstract)



[14]Wang R-F(王如芳), Zhang J-W(张吉旺), Lü P(吕鹏), Dong S-T(董树亭), Liu P(刘鹏), Zhao B(赵斌). Tillering characteristics of multi-tiller maize and influence of plant density and sowing date. Acta Agron Sin (作物学报), 2012, 38(2): 322−332 (in Chinese with English abstract)



[15]Liu Y(刘杨), Ding Y-F(丁艳锋), Wang Q-S(王强盛), Li G-H(李刚华), Xu J-X(许俊旭), Liu Z-H(刘正辉), Wang S-H(王绍华). Effect of plant growth regulators on the growth of rice tiller bud and the changes of endogenous hormones. Acta Agron Sin (作物学报), 2011, 37(4): 670−676 (in Chinese with English abstract)



[16]Liu Y(刘杨), Wang Q-S(王强盛), Ding Y-F(丁艳锋), Liu Z-H(刘正辉), Li G-H(李刚华), Wang S-H(王绍华). Effect of nitrogen and 6-BA on development of tillering bud and its physiological mechanism. Acta Agron Sin (作物学报), 2009, 35(10): 1893−1899 (in Chinese with English abstract)



[17]Ma X-L(马兴林), Liang Z-X(梁振兴). Studies on the effects of endogenous hormones in winter wheat tillers during the course of senescence. Acta Agron Sin (作物学报), 1997, 23(2): 200−207 (in Chinese with English abstract)



[18]Li C-X(李春喜), Zhao G-C(赵广才), Dai X-M(代西梅), Jiang L-N(姜丽娜), Shang Y-L(尚玉磊). Research on the relationship between wheat tillering dynamics and endogenous hormone. Acta Agron Sin (作物学报), 2000, 26(6): 963−968 (in Chinese with English abstract)



[19]Yang T-X(杨途熙), Wei A-Z(魏安智), Zheng Y(郑元), Yang H(杨恒), Yang X-N(杨向娜), Zhang R(张睿). Simultaneous determination of endogenous hormone sin apricot floral bud by high performance liquid chromatography. Chin J Anal Chem (分析化学), 2007, 35(9): 1359−1361 (in Chinese with English abstract)



[20]Yang W-B(杨卫兵), Wang Z-L(王振林), Yin Y-P(尹燕枰), Li W-Y(李文阳), Li Y(李勇), Chen X-G(陈晓光), Wang P(王平), Chen E-Y(陈二影), Guo J-X(郭俊祥), Cai T(蔡铁), Ni Y-L(倪英丽). Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains. Sci Agric Sin (中国农业科学), 2011, 44(13): 2673−2682 (in Chinese with English abstract)



[21]Liu Y, Ding Y F, Wang Q S, Meng D X, Wang S H. Effects of nitrogen and 6-Benzylaminopurine on rice tiller bud growth and changes in endogenous hormones and nitrogen. Crop Sci, 2011, 51: 786–792



[22]Wang Y-S(王永胜), Wang J(王景), Duan J-Y(段静雅), Wang J-F(王金发), Liu L-S(刘良式). Isolation and genetic research of a dwarf tillering mutant rice. Acta Agron Sin (作物学报), 2002, 28(2): 235−239 (in Chinese with English abstract)



[23]Tang J-B(唐家斌), Zeng W-Y(曾万勇), Wang W-M(王文明), Ma B-Q(马炳田), Liu Y(刘勇), Li H-J(李浩杰), Xia H-A(夏红爱), Li P(李平), Zhu L-H(朱立煌). Genetic research and genetic supports of a few tiller mutant rice. Sci China (Ser C: Life Sci)(中国科学•C辑), 2001, 31(3): 208−212 (in Chinese)



[24]Yang W-Y(杨文钰), Han H-F(韩惠芳), Ren W-J(任万君), Zhao L(赵莉), Fan G-Q(樊高琼). Effects of uniconazole waterless-dressing seed on endogenous hormones and C/N ratio at tillering stage of wheat. Acta Agron Sin (作物学报), 2005, 31(6): 760−765 (in Chinese with English abstract)



[25]Wang S-H(王绍华), Jie S-T(揭水通), Ding Y-F(丁艳锋), Wang Q-S(王强盛). Effects of tiller inhibitors on regulation of rice tillering. Jiangsu J Agric Sci (江苏农业学报), 2002, 18(1): 29−32 (in Chinese with English abstract)



[26]Zhou C-F(周传凤), Li Y-R(李杨瑞), Yang L-T(杨丽涛). Effect of ethephon sprayed at early tillering stage on the activities of peroxidase, IAA oxidase and acid invertase in sugarcane in correlation to tillering. Guihaia (广西植物), 2007, 27(4): 649−652 (in Chinese with English abstract)



[27]Woodward E, Marshall C. Effects of plant growth regulators and nutrient supply on tiller bud outgrowth in barley (Hordeum distichum L.). Ann Bot, 1998, 61: 347−354



[28]Morris G C, Choonseok O H. A reappraisal of the role of abscisic acid and its interaction with auxin in apical dominance. Ann Bot, 2006, 98: 891−897



[29]Michael G, Beringer H. The role of hormones in yield formation, physiological aspects of group productivity. In: Proceedings of the Colloquium of the International Potash Institute, Wageninien, The Netherlands, 1980. pp 252−279



[30]Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H. Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J, 2006, 45: 1028−1036 



[31]Bangerth F. Response of CK concentration in the xylem exudates of bean (Phaseolus vulgaris L.) plants to decapitation and auxin treatment, and relationship to apical dominance. Planta, 1994, 194: 439−442



[32]Liang Z-X(梁振兴), Ma X-L(马兴林). Studies on the effects of endogenous hormones on tiller development process of winter wheat. Acta Agron Sin (作物学报), 1998, 24(6): 788−792 (in Chinese with English abstract)



[33]Cline M G. Exogenous auxin effects on lateral bud outgrowth in decapitated shoots. Ann Bot, 1996, 78: 256−266



[34]Cline M G, Chatfield S P, Leyser O. NAA restores apical dominance in the axr3-1 mutant of Arabidopsis thaliana. Ann Bot, 2001, 87: 61−65



[35]Shang Y-L(尚玉磊), Li C-X(李春喜); Shao Y(邵云), Jiang L-N(姜丽娜). Comparison of dynamics and functions of endogenous IAA, CTK content among main crops of gramineae at early growing stage. Acta Agricul Boreali-Sin (华北农学报), 2004, 19(4): 47−50 (in Chinese with English abstract)



[36]Zhang Z-D(张祖德). Research of chemical regulation on promote the panicle bearing tiller rate of rice. Fujian Sci Tech Rice Wheat (福建稻麦科技), 2006, (6): 10−13 (in Chinese)



[37]Chrispeels M, Varner J. Inhibition of gibberellic acid induced formation of α-amylase by abscisin II. Nature, 1966, 212: 1066−1067



[38]Pei Z M, Ghassemian M, Kwak C M. Role of farnesyltransferase in ABA regulation of guard cell anion channels and plant water loss. Science, 1998, 282: 287−290



[39]Wang G-M(王光明), Liu B-G(刘保国), Chen J(陈静), Ren C-F(任昌福). Effects of endogenous ABA on the germination of ratooning buds in paddy rice. J Southwest Agric Univ (西南农业大学学报), 1997, 19(4): 338−342 (in Chinese with English abstract)



[40]Xu L-N(徐丽娜), Feng W(冯伟), Sheng K(盛坤), Zhu Y-J(朱云集), Ma D-Y(马冬云), Guo T-C(郭天财) Difference of endogenous hormones in Leaves between main stem and tillers and its effect on spike formation in large spike winter wheat cultivar Lankao Aizao 8 under different plant densities. Acta Agron Sin (作物学报), 2010, 36(9): 1596−1604 (in Chinese with English abstract)

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